A5026101627- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Tracheal and airway disorders
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Neuroscience and Neuropharmacology Research
- Respiratory viral infections research
- Fatty Acid Research and Health
- Respiratory Support and Mechanisms
- Hormonal Regulation and Hypertension
- Cardiac electrophysiology and arrhythmias
- Neuroscience of respiration and sleep
- Infant Nutrition and Health
- Barrier Structure and Function Studies
- Immune cells in cancer
- Stress Responses and Cortisol
- Cardiac Fibrosis and Remodeling
- Neuroscience and Neural Engineering
- Sulfur Compounds in Biology
- Adenosine and Purinergic Signaling
- Electrolyte and hormonal disorders
- Pancreatic function and diabetes
Inserm
2013-2024
Université Paris-Est Créteil
2022-2024
Institut Necker Enfants Malades
2015-2022
Institut Mondor de Recherche Biomédicale
2008-2022
The University of Texas Southwestern Medical Center
2021
Victor Babeș University of Medicine and Pharmacy Timișoara
2021
Centre National de la Recherche Scientifique
2015-2019
National Children’s Research Centre
2012-2017
Royal College of Surgeons in Ireland
2011-2017
Beaumont Hospital
2011-2017
Clinical studies with modulators of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) protein have demonstrated that functional restoration mutated CFTR can lead to substantial clinical benefit. However, shown highly variable patient responses. The objective this study was determine a biomarker predictive response. function assessed in vivo via nasal potential difference (NPD) and human epithelial (HNE) cultures by response Forskolin/IBMX potentiator VX-770 short-circuit-current...
Abstract Cystic fibrosis (CF) is caused by defective Fibrosis Transmembrane Conductance Regulator (CFTR) protein. Morbidity mainly due to early airway infection. We hypothesized that S. aureus clearance during the first hours of infection was impaired in CF human Airway Surface Liquid (ASL) because a lowered pH. The ASL pH bronchial epithelial cell lines and primary respiratory cells from healthy controls (WT) patients with measured microelectrode. antimicrobial capacity studied after apical...
Airway disease in cystic fibrosis (CF) is characterised by impaired mucociliary clearance, persistent bacterial infection and neutrophilic inflammation. Lipoxin A4 (LXA4) initiates the active resolution of inflammation promotes airway surface hydration CF models. 15-Lipoxygenase (LO) plays a central role "class switch" eicosanoid mediator biosynthesis from leukotrienes to lipoxins, initiating We hypothesised that defective class switching contributes failure resolve lung disease. Using...
The specialized proresolution lipid mediator lipoxin A4 (LXA4) is abnormally produced in cystic fibrosis (CF) airways. LXA4 increases the CF airway surface liquid height and stimulates epithelial repair tight junction formation. We report here a protective effect of (1 nM) against disruption caused by Pseudomonas aeruginosa bacterial challenge together with delaying action invasion cells from patients immortalized cell lines. Bacterial integrity were measured gentamicin exclusion assays...
In cystic fibrosis (CF), impaired mucociliary clearance leads to chronic infection and inflammation. However, cilia beating features in a CF altered environment, consisting of dehydrated airway surface liquid layer abnormal mucus, have not been fully characterized. Furthermore, acute inflammation is normally followed by an active resolution phase requiring specialized proresolving lipid mediators (SPMs) allowing return homeostasis. SPMs biosynthesis has reported CF. Here, we explored...
Lipoxin A(4) (LXA(4)) is a biologically active eicosanoid produced in human airways that displays anti-inflammatory properties. In cystic fibrosis and severe asthma, LXA(4) production has been reported to be decreased, and, such diseases, one of the consequences airway inflammation disruption tight junctions. present study, we investigated possible role on junction formation, using transepithelial electrical resistance (TER) measurements, Western blotting, immunofluorescence. We observed...
Cystic Fibrosis (CF) is a genetic disease characterised by deficit in epithelial Cl− secretion which the lung leads to airway dehydration and reduced Airway Surface Liquid (ASL) height. The endogenous lipoxin LXA4 member of newly identified eicosanoids playing key role ending inflammatory process. Levels are reported be decreased airways patients with CF. We have previously shown that normal human bronchial cells, produced rapid transient increase intracellular Ca2+. investigated, effect on...
The main cause of morbidity and mortality in cystic fibrosis (CF) is progressive lung destruction as a result persistent bacterial infection inflammation, coupled with reduced capacity for epithelial repair. Levels the anti-inflammatory mediator lipoxin A₄ (LXA₄) have been reported to be bronchoalveolar lavages patients CF. We investigated ability LXA₄ trigger repair through initiation proliferation migration non-CF (NuLi-1) CF (CuFi-1) airway epithelia. Spontaneous cell were significantly...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Key points Oestrogen (E 2 ) exposure leads to a decrease in both Cl − secretion and KCNQ1 current. This inhibition is maintained by rapid sustained retrieval of the channel from plasma membrane. The E ‐stimulated internalization occurs via dynamin‐ clathrin‐dependent mechanism. recycled back cell membrane Rab4 Rab11 rather than being degraded. signalling pathway activated leading involves cascade, which activation protein kinase Cδ induces phosphorylation AMP‐dependent kinase. stimulated an...
A non‐genomic antisecretory role for dexamethasone at low concentrations (0.1 n m to1 μ ) is described in monolayers of human bronchial epithelial cells primary culture and a continuous cell line (16HBE14o‐ cells). Dexamethasone produced rapid decrease [Ca 2+ ] i (measured with fura‐2 spectrofluorescence) to new steady‐state concentration. After 15 min exposure (1 ), was reduced by 32 ± 11 ( = 7 , P < 0.0001 from basal value 213 36 ). We have shown previously that aldosterone also...
Lipoxins are biologically active eicosanoids possessing anti-inflammatory properties. Using a calcium imaging system we investigated the effect of lipoxin A4 (LXA4) on intracellular [Ca2+] ([Ca2+]i) human bronchial epithelial cell. Exposure cells to LXA4 produced dose-dependent increase in [Ca2+]i followed by recovery basal values primary culture and 16HBE14o− cells. The LXA4-induced was completely abolished after pre-treatment with pertussis toxin (G-protein inhibitor). response not...
Thiazolidinediones (TZDs) are synthetic peroxisome proliferator-activated receptor-gamma (PPARgamma) ligands that widely used in type II diabetes treatment. In addition to their ability improve glucose homeostasis, TZDs possess anti-inflammatory properties and inhibit growth of many cells, particularly cancerous airway epithelial cells. However, the functional effects PPARgamma on nonmalignant human bronchial cells have never been investigated. present study, we questioned whether may...
Non‐Technical Summary High levels of oestrogen are known to cause fluid retention in fertile females. It is thought that the increase body volume necessary for proper implantation fertilised egg uterus. We show activity a potassium ion channel, which drives salt and water movement across cell membranes intestine, inhibited by this effect only found females maximal during peak phase oestrous cycle (when fertilization occur). These findings help us understand molecular mechanisms underlying...
In cystic fibrosis (CF), the airway surface liquid (ASL) is depleted. We previously demonstrated that lipoxin A4 (LXA4) can modulate ASL height (ASLh) through actions on Cl(-) transport. Here, we report novel effects of epithelial Na(+) channel ENaC in this response. dynamics and ion transport were studied using live-cell confocal microscopy short-circuit current measurements CF (CuFi-1) non-CF (NuLi-1) cell cultures. Low physiological concentrations LXA4 picomolar range produced an increase...
1. In epithelial cells of frog skin, potassium ions are recycled across the basolateral membrane via an inward-rectifier, ATP-sensitive K+ channel (KATP channel). this study, we show that aldosterone has a stimulatory effect on KATP activity and have investigated involvement Na+-H+ exchange intracellular pH (pHi) in phenomenon. 2. Aldosterone (10 nM) produced increase open probability within 15 min from 0.21 +/- 0.05 to 0.93 0.10 (n = 8), measured cell-attached patches. also increased...
Glucocorticoids have been shown to produce rapid nongenomic responses in airway epithelia. By using an intracellular pH (pH(i)) spectrofluorescence imaging system and the NH4Cl acid-loading technique, we that synthetic glucocorticoid,dexamethasone, accelerated recovery after acid load a human bronchial epithelial cell line (16HBE14o- cells). Exposure (20 mm) elicited acidification, followed by pH(i) recovery. Inhibition of Na+/H+ exchanger decreased steady-state antagonized dexamethasone...