One of the main factors that influence fertility cattle in grazing systems hot tropical climates is heat stress. The objective this study was to evaluate effect season, breed, hormonal and physiological condition on quantity quality cumulus-oocyte complexes (COCs) embryos produced vitro, from Romosinuano (RM) Tropical Milking (TM) donors. Three ovum pick-up vitro fertilization (OPU-IVF) were performed, one per season; dry (HD; 10, 10), humid (HH; 9, 9) fresh (FD; 7, 10) RM LT Serum levels...

The aim of this study was to quantify the content lipid droplets in bovine oocytes and embryos from Bos indicus (Bi), taurus (Bt) × (Bi Bt). Oocytes were aspirated post-mortem subjected vitro maturation, fertilization development; medium employed at each stage (TCM-199, TALP, SOF) supplemented with (i) serum replacement (SR), (ii) foetal calf (FCS) or (iii) oestrous cow (ECS). structure distribution established using electron microscopy, but quantified an optical microscope on semi-fine...

A decrease in fertility can have a negative economic impact, both locally and over broader geographical scope, this is especially the case with regard to cattle industry. Therefore, much interest exists evaluating proteins that might be able increase of sperm. Heparin binding (HBPs), specifically associated antigen (FAA) Type-2 tissue inhibitor metalloproteinase (TIMP-2), act favor capacitation acrosome reaction perhaps even modulate immune system’s response toward The objective research was...

One of the main factors that influences fertility cattle in grazing systems hot tropical climates is heat stress. The objective this study was to evaluate effect season, breed, hormonal and physiological condition on quantity quality cumulus–oocyte complexes (COCs) embryos produced vitro, from Romosinuano (RM) Tropical Milking (TM) donors. Three ovum pick-up vitro fertilization (OPU-IVF) were performed, one per season: dry (HD; 10, 10), humid (HH; 9, 9) fresh (FD; 7, 10) RM LT Serum levels...

Carbohydrate residues on membrane proteins from sperm are important in gamete interaction. In recent years, Arylsulfatase A (AS-A) has been acquiring an role the various putative interaction responsibles sperm. The aim of this study was to determine if capacitated boar Arylsulfatase-A (AS-A), contains D-mannose, N-acetylglucosamine and/or sialic acid by its purification using affinity chromatography with Concanavalia ensiformis Agglutinin(Con-A) or Wheat Germ Agglutinin (WGA) as ligands....

In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was determine efficiency fertilization using ICSI with chemically activated ovine oocytes selected by swim up (SU) or + zona pellucida (SU ZP) binding.Experiment 1, 4-20 replicates total 821 in vitro matured were ethanol, calcium ionophore ionomycin, (precense one PN). Treatments showed similar results (54, 47, 42 %, respectively) but statistically differents (P < 0.05)...

The use of reproductive technologies such as somatic cell nuclear transfer (SCNT) for avian species has been limited by the inability to visualise pronucleus or pronuclei within blastodisc germinal disc region, respectively, primarily due opacity large, lipid filled yolk. main objective in present study was assess a method visualising and enucleating ovum, critical step developing capability cloning birds. utilised investigation epi-fluorescence transmitted light (top-side UV) microscopy...

Chitosan is a partially deacetylated polymer obtained from the alkaline deacetylation of chitin, which glucose-based unbranched polysaccharide widely distributed in nature as main component exoskeletons crustaceans and insects. has variety physicochemical biological properties resulting numerous applications. In addition to its lack toxicity allergenicity, biocompatibility, biodegradability, bioactivity make it very attractive substance for diverse applications biomaterial pharmaceutical...

The decrease in the fertility of males can have a negative economic impact on production milk and meat cattle. As result, interest exists evaluating diverse proteins that might be able to increase sperm. This is case heparin-binding (HBP), specifically fertility-associated antigen (FAA) Type-2 tissue inhibitor metalloproteinase (TIMP-2), which act favour capacitation acrosome reaction, perhaps by modulating immune system response toward objective this study was evaluate effect adding...

There is a need to develop an efficient cryopreservation technique for Brahman cattle embryos that would lead improved success in the propagation of this breed. The objective study was compare post-thaw pregnancy rates recipient cows after nonsurgical transfer vivo-derived frozen ethylene glycol (EG) and by new vitrification (VT) method. Prior initial vivo study, hatching were recorded 72 h on 3 treatment groups (No treatment: 43/55, 78%; EG: 39/83, 47%; VT: 33/103, 32%) IVF determine if...

Abstract The pterostilbene (PT) molecule is a phytoalexin with reducing effect on reactive oxygen species (ROS) and capacity to block lipogenesis. However, the potential effects of PT equatorial lipid accumulation ROS have not yet been elucidated for in vitro‐derived bovine embryos. present study evaluated concentrations 3, 1, 0.33, 0.11 μM PT, vehicle group percentage cleaved embryos, embryos more than 6 cells, blastocyst Day 7 8, transferable 7, cell count relative concentration lipids. In...

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

High levels of lipid within in vitro-produced embryos during freezing can increase intracellular damage and lower production rates (Seidel 2006 Theriogenology 65, 228). The objective this study was to determine if segregation with or without laser-assisted hatching (LAH), zona pellucida drilling vitro-fertilized (IVF) would enhance vitro survivability development 24 h post-thaw. Three replicates utilizing 1179 bovine oocytes (BOMED, Madison, WI, USA) were fertilized frozen/thawed Tuli bull...

The present objective was to determine if interspecies somatic cell nuclear transfer (SCNT) could be a viable option for producing cloned Rocky Mountain bighorn sheep (RMBS) embryos, model other endangered ovine species or of high genetic value. A primary question whether cells from harvested 5.5-year-old male RMBS (Ovis canadensis canadensis) cultured 4 days postmortem SCNT embryo production. Domestic ovaries an abattoir were placed in TCM199 holding medium containing Hanks’ salts, kept at...

Objective: Evaluate the White-tailed Deer (WTD) in vitro embryo production (IVP) and oocytes vitrified with Trehalose (TH) or Sucrose (SC). Design/methodology/approach: Total were placed into two different groups: TH (n=60) SC (n=61). Samples selected analyzed for viability evaluation (n=5) (n=5), nuclear status (NS) (n=4) Germinal Vesicle (GV), Metaphase I, not evaluable (NE) after warming. In maturation (IVM) was conducted 36 h supplemented TCM-199 medium. Immediately afterwards, oocyte NS...

In vitro production of germline chimeras and avian cloning may utilise the transfer embryos from their original eggshell to a surrogate for culture during incubation. Such embryo is valuable as only alternative would be cloned into infundibulum recipient birds. Given advances in paleogenomics, synthetic biology, gene editing, similar approach might used generate extinct species, i.e. de-extinction. One objective present research was examine if ratite eggs could manipulated via windowing sham...

SexedULTRA-4M™ is made using an improved method of sex-sorting sperm in a less damaging environment for better retaining integrity throughout the sorting process. The objective this research was to compare conventional (CONV) and (ULTRA-4M) semen bovine IVP four Angus bulls. Matured slaughterhouse oocytes (n = 4000) were divided into CONV group ULTRA-4M (2000 COCs each type). IVF process implemented with from same bull. cleavage rates, eight cell embryos blastocysts on day 7 culture...