A5056354861- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Sperm and Testicular Function
- Effects of Environmental Stressors on Livestock
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Renal and related cancers
- Ruminant Nutrition and Digestive Physiology
- Molecular Biology Techniques and Applications
- Prenatal Screening and Diagnostics
- Heat shock proteins research
- Animal Disease Management and Epidemiology
- Heat Transfer Mechanisms
- Ovarian function and disorders
- Microbial infections and disease research
- Growth Hormone and Insulin-like Growth Factors
- Thermoregulation and physiological responses
- Adipose Tissue and Metabolism
- Meat and Animal Product Quality
- Epigenetics and DNA Methylation
- Muscle Physiology and Disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Tissue Engineering and Regenerative Medicine
University of Wisconsin–Madison
2024
University of Florida
2019-2023
University of Kansas Medical Center
2022-2023
Cornell University
2018-2023
University of Kansas
2022
Universidad de Cuautitlán Izcalli
2016-2020
The SLICK1 mutation in bovine PRLR (c.1382del; rs517047387) is a deletion resulting protein with truncated intracellular domain. Cattle carrying at least one allele have phenotype characterized by short hair coat (slick phenotype) and increased resistance to heat stress. Given the pleiotropic nature of prolactin, may affect other physiological characteristics. liver organ that could potentially be affected because expression PRLR. dominant allele, heterozygous animals similar homozygous for...
Nutrient starvation drives yeast meiosis, whereas retinoic acid (RA) is required for mammalian meiosis through its germline target Stra8. Here, by using single-cell transcriptomic analysis of wild-type and Stra8-deficient juvenile mouse germ cells, our data show that the expression nutrient transporter genes, including Slc7a5, Slc38a2, Slc2a1, downregulated in cells during meiotic initiation, this process requires Stra8, which binds to these genes induces their H3K27 deacetylation....
Inheritance of the SLICK1 allele prolactin receptor gene improves thermotolerance lactating Holstein cows under humid heat stress conditions. The aim this study was to investigate whether pre- and postweaning heifers carrying would show physiological responses indicative higher tolerance in high- low-humidity climates. A total 101 heifer calves two age groups heterozygous for 103 wild-type half-siblings were evaluated during July 2020 3 dairy farms central California 2 south Florida. Dry...
Our objective was to compare reproductive outcomes of primiparous lactating Holstein cows different genetic merit for fertility submitted insemination with management programs that prioritized artificial (AI) at detected estrus (AIE) or timed AI (TAI). Moreover, we aimed determine whether subgroups potential would present a distinct response the strategies compared. Lactating (n = 6 commercial farms) were stratified into high (Hi-Fert), medium (Med-Fert), and low (Lo-Fert) groups (FG) based...
The slick-hair phenotype in cattle is due to one of a series mutations the prolactin receptor (PRLR) that cause truncation C-terminal region protein involved JAK2/STAT5 activation during signaling. Here we evaluated whether inheritance SLICK1 allele, first slick mutation discovered, inherited fashion consistent with Hardy-Weinberg equilibrium. It was hypothesized any deleterious effect inheriting allele on embryonic or fetal function would result reduced frequency offspring. A total 525...
ABSTRACT Bovine embryonic stem cells (ESC) have features associated with the primed pluripotent state including low expression of one core pluripotency transcription factors, NANOG. It has been reported that NANOG can be upregulated in porcine ESC by treatment activin A and WNT agonist CHIR99021. Accordingly, it was tested whether another factor SOX2 could stimulated Immunoreactive were analyzed for bovine lines derived under conditions which CHIR99021 added singly or combination. Activin...
Abstract Background Colony-stimulating factor 2 (CSF2) is an important maternal regulator of embryonic development. Earlier research indicates that CSF2 can regulate genes involved in cellular stress responses and block apoptosis. Here, we tested whether addition 10 ng/mL at day 5 development would increase the survival blastocysts harvested 7 subjected to vitrification. Additional objectives were determine embryo sex affected or effects interacted with sex. Results Survival after...
The SLICK1 mutation in the prolactin receptor (PRLR) results a short-hair coat and increased ability to regulate body temperature during heat stress. It is unclear whether affects capacity for sweating. objective of this observational study was evaluate PRLR alters characteristics skin related sweat gland abundance or function. Skin biopsies from 31 Holstein heifers, including 14 wild-type (SL−/−) 17 heterozygous slick (SL+/−), were subjected histological analysis determine percent surface...
The hypothesis that CSF2 plays a role in the preimplantation development of bovine embryo was tested by evaluating consequences inactivation CSF2RA (the functional receptor embryo) for embryos utero. CRISPR/Cas9 used to alter sequences on exon 5 and intron CSF2RA, Control were injected with Cas9 mRNA only. Embryos > 16 cells at day after insemination transferred synchronized recipient females groups 7 24. flushed from uterus two days later. proportion recovered developed blastocyst stage...
Summary Heat stress can have severe deleterious effects on embryo development and survival. The present study evaluated whether CSF2 protect the developmental competence of bovine following exposure to a heat shock 41°C at zygote morula stages. In first experiment, putative zygotes 2-cell embryos were assigned receive either 10 ng/ml or vehicle, then cultured for 15 h 38.5°C until day 7.5. reduced blastocyst treated with vehicle but not CSF2. second 5 (morula) Temperature treatment did...
Gene expression analysis in preimplantation embryos has been used for answering fundamental questions related to development, prediction of pregnancy outcome, and other topics. Limited amounts mRNA hinders progress studying the embryo. Here, a method was developed involving direct synthesis specific-target preamplification (STA) cDNA gene single blastocysts. Effective cell lysis genomic DNA removal steps were incorporated into method. In addition, conditions real-time PCR generated from...
Abstract The pterostilbene (PT) molecule is a phytoalexin with reducing effect on reactive oxygen species (ROS) and capacity to block lipogenesis. However, the potential effects of PT equatorial lipid accumulation ROS have not yet been elucidated for in vitro‐derived bovine embryos. present study evaluated concentrations 3, 1, 0.33, 0.11 μM PT, vehicle group percentage cleaved embryos, embryos more than 6 cells, blastocyst Day 7 8, transferable 7, cell count relative concentration lipids. In...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
The objectives of the experiment were to determine effect two doses equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on short-term progesterone (P4 ) priming ovarian structures and haemodynamics, concentrations steroid hormones prolificacy rate when oestrus was induced during low-breeding season (LBS) Beetal dairy goats. We hypothesized that inclusion eCG P4 priming-based would increase blood perfusion leading enhance oestrous ovulatory responses Forty-two...
The actual challenge for the majority of research centers involves embryo culture medium, since it is known that medium plays a large role in determining quality. Pterostilbene (PT) natural analogue Resveratrol, an antioxidant can reduce lipids embryos, but no reports exist PT being used with IVF-produced embryos or gametes. objective present was to evaluate effect media CDM1 and CDM2 on production, cell count, lipid accumulation, reactive oxygen species (ROS). A total 4 concentrations...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Colony-stimulating factor 2 (CSF2) is an important maternal regulator of embryonic development. Treatment invitro-produced embryos with CSF2 from Days 5 to 7 after insemination increased competence establish pregnancy transfer. One action protect stress. reduced heat-shocked induced apoptosis in morulae and regulated expression multiple genes involved cellular stress responses the blastocyst. Here we tested whether would promote survival another stress: cryodamage caused by vitrification....
This protocol for using PCR to sex bovine embryos are based on Y-specific and autosomal targets described by Park et al, Theriogenology 2001; 55: 1843-1853 but with updates according current genomic database in NCBI as of 2019.Note that assembly the Y chromosome is still progress.
This is a simple protocol for collecting skin biopsies from cattle. The procedure described here was used to collect histological analysis but it could also be PCR or other purposes. Our experience indicates that there are few complications with the procedure. most common occasionally slight amount of bleeding can treated by applying pressure using gauze. Healing occurs rapidly and biopsy sites difficult detect 14 days after best read attached pdf file.