A5014864011- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Reproductive Physiology in Livestock
- Effects of Environmental Stressors on Livestock
- Growth Hormone and Insulin-like Growth Factors
- Milk Quality and Mastitis in Dairy Cows
- Molecular Biology Techniques and Applications
- Meat and Animal Product Quality
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Infant Nutrition and Health
- Reproductive Biology and Fertility
- Animal Virus Infections Studies
- Adipose Tissue and Metabolism
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Agricultural and Food Production Studies
- Bacterial Identification and Susceptibility Testing
- Cholesterol and Lipid Metabolism
- Ruminant Nutrition and Digestive Physiology
- Genomic variations and chromosomal abnormalities
- Metabolism, Diabetes, and Cancer
Zoetis (United States)
2014-2024
Genus (United States)
2022-2024
University of California, Davis
2007-2016
University of Alaska Fairbanks
2014
Delta Air Lines (United States)
2014
Montclair State University
2014
University of California System
2012-2013
Universidad de la República
2006
Universidad de Montevideo
2006
University of Zulia
2001
Abstract Background Cow milk is a complex bioactive fluid consumed by humans beyond infancy. Even though the chemical and physical properties of cow are well characterized, very limited research has been done on characterizing transcriptome. This study performs comprehensive expression profiling genes expressed in somatic cells transition (day 15), peak 90) late 250) lactation Holstein cows RNA sequencing. Milk samples were collected from at 15, 90 250 days lactation, was extracted pelleted...
Disease resistance genes in livestock provide health benefits to animals and opportunities for farmers meet the growing demand affordable, high-quality protein. Previously, researchers used gene editing modify porcine CD163 demonstrated a harmful virus that causes reproductive respiratory syndrome (PRRS). To maximize potential benefits, this disease trait needs be present commercially relevant breeding populations multiplication distribution of pigs. Toward goal, first-of-its-kind, scaled...
The newly assembled Bos taurus genome sequence enables the linkage of bovine milk and lactation data with other mammalian genomes.Using publicly available proteome mammary expressed tags, 197 protein genes over 6,000 were identified in genome. Intersection these 238 production quantitative trait loci curated from literature decreased search space for effectors by more than an order magnitude. Genome location analysis revealed a tendency to be clustered genes. Using genomes monotreme...
High-throughput sequencing of RNA (RNA-Seq) was developed primarily to analyze global gene expression in different tissues. However, it also is an efficient way discover coding SNPs. The objective this study perform a SNP discovery analysis the milk transcriptome using RNA-Seq. Seven samples from Holstein cows were analyzed by cDNAs Illumina Genome Analyzer system. We detected 19,175 genes expressed corresponding approximately 70% total number analyzed. detection revealed 100,734 SNPs...
The objective of this study was to examine five different sources RNA, namely mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected epithelial (LCMEC), fat globules (MFG) and antibody-captured (mMEC) analyze the bovine transcriptome using RNA-Sequencing. Our results provide a comparison between sampling methods (invasive non-invasive) define cells. This information will be value investigators in choosing most appropriate method for research applications specific...
Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding the genes and regulatory pathways networks involved in puberty, we characterized transcriptome five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, endometrium) as well known be relevant growth metabolism needed achieve puberty (i.e., longissimus dorsi muscle, adipose, liver). These were collected from pre- post-pubertal...
Improvement in feed conversion efficiency can improve the sustainability of beef cattle production, but genomic selection for affects many underlying molecular networks and physiological traits. This study describes differences between steer progeny two influential Angus bulls with divergent predictions residual intake (RFI). Eight each sire were phenotyped growth from 8 mo. age (average BW 254 kg, a mean difference groups 4.8 kg) until slaughter at 14–16 534 group 28.8 kg). Terminal samples...
Genes in the sterol regulatory element-binding protein-1 (SREBP1) pathway play a central role regulation of milk fat synthesis, especially de-novo synthesis saturated fatty acids. SCD, SREBP-responsive gene, is key enzyme monounsaturated acids mammary gland. In present study, we discovered SNP candidate genes associated with this signalling and SCD to identify genetic markers that can be used for metabolically directed selection cattle. We resequenced six SREBP1 (SREBP1, SCAP, INSIG1,...
Abstract Background Use of RNA-Seq presents unique benefits in terms gene expression analysis because its wide dynamic range and ability to identify functional sequence variants. This technology provides the opportunity assay developing embryo, but paucity biological material available from individual embryos has made this a challenging prospect. Results We report here first application for blastocyst expression, SNP detection, characterization allele specific (ASE). RNA was extracted single...
Thermal exposure is a serious and growing challenge facing fish species worldwide. Chinook salmon (Oncorhynchus tshawytscha) living in the southern portion of their native range are particularly likely to encounter warmer water due confluence factors. River alterations have increased likelihood that juveniles will be exposed warm temperatures during freshwater life stage, which can negatively impact survival, growth, development pose threat dwindling populations. To better understand how...
This study examines the genes coding for enzymes involved in bovine milk oligosaccharide metabolism by comparing profiles with expressions of glycosylation-related genes. Fresh samples (n = 32) were collected from four Holstein and Jersey cows at days 1, 15, 90 250 lactation free analyzed. RNA was extracted somatic cells 15 12) gene expression analysis conducted RNA-Sequencing. A list created 121 pathways analyzing performing an extensive literature search. No significant differences...
Measures of heifer fertility are economically relevant traits for beef production systems and knowledge candidate genes could be incorporated into future genomic selection strategies. Ten related to growth were measured in 890 Brangus heifers (3/8 Brahman × 5/8 Angus, from 67 sires). These were: BW hip height adjusted 205 365 d age, postweaning ADG, yearling assessment carcass (i.e., back fat thickness, intramuscular fat, LM area), as well pregnancy first service conception (FSC). collected...
Identification of the genes and polymorphisms underlying quantitative traits, understanding how these affect economic are important for successful marker-assisted selection more efficient management strategies in commercial cattle populations. Signal transducer activator transcription 6 (STAT6) gene is tightly connected to IL-4 IL-13 signalling plays a key role T(H)2 polarization immune system. In addition, STAT6 acts as mediator leptin has been associated with body weight regulation. The...
Abstract Background The accurate identification of the functional elements in bovine genome is a fundamental requirement for high-quality analysis data informing both biology and genomic selection. Functional annotation was performed to identify more complete catalog transcript isoforms across tissues. Results A total 160,820 unique transcripts (50% protein coding) representing 34,882 genes (60% were identified Among them, 118,563 (73% total) structurally validated by independent datasets...
The growth endocrine axis influences reproduction. objectives of this study were to evaluate population genetic characteristics SNP genotypes within genes the GH-IGF in straightbred and crossbred Angus, Brahman, Romosinuano heifers (n = 650) test association these with measures These achieved using 73 7 on chromosome 5 pregnancy-associated plasma protein A2 (PAPP-A2) GH-receptor genes, which map chromosomes 16 20, respectively. elucidated by resequencing conserved regions each gene DNA from...
In a marker-trait association study we estimated the statistical significance of 65 single nucleotide polymorphisms (SNP) in 23 candidate genes on HDL levels two independent Caucasian populations. Each population consisted men and women their were adjusted for gender body weight. We used linear regression model. Selected corresponded to folate metabolism, vitamins B-12, A, E, cholesterol pathways or lipid metabolism. Extracted DNA from both Sacramento Beltsville populations was analyzed...
The aim of this study was to investigate the genetic diversity within and among three breeds sheep: Corriedale, Merino Creole. Sheep from (Merino n = 110, Corriedale 108 Creole 10) were genotyped using Illumina Ovine SNP50 beadchip®. Genetic evaluated by comparing minor allele frequency (MAF) breeds. Population structure differentiation assessed STRUCTURE software, principal component analysis (PCA) fixation index (F ST). Fixed markers (MAF 0) that different identified as specific breed...
Abstract The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence establish and maintain gestation. Embryos produced X-sorted sperm were cultured from day 5 7 serum-free medium containing 10 ng/ml recombinant bovine colony-stimulating factor 2 (CSF2) or vehicle. CSF2 administered because this molecule can increase blastocyst for survival after embryo transfer. Blastocysts harvested on culture manually bisected. One demi-embryo a single...