A5054552428- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Effects of Environmental Stressors on Livestock
- Birth, Development, and Health
- Assisted Reproductive Technology and Twin Pregnancy
- Global Cancer Incidence and Screening
- Economic and Financial Impacts of Cancer
- Ovarian function and disorders
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Xenotransplantation and immune response
- Pluripotent Stem Cells Research
- COVID-19 and healthcare impacts
- Seed Germination and Physiology
- Growth Hormone and Insulin-like Growth Factors
- Heat shock proteins research
- Image Processing and 3D Reconstruction
- Gestational Diabetes Research and Management
- Bioenergy crop production and management
- Fatty Acid Research and Health
- Forest Ecology and Biodiversity Studies
University of Wyoming
2021-2024
Division of Undergraduate Education
2021-2023
Institute of Developmental Physiology
2023
University of Florida
2011-2022
Advatech Pacific (United States)
2014-2022
Wyoming Department of Education
2021
Zoetis (United States)
2019-2020
In this study, we tested the role of colony-stimulating factor 2 (CSF2) as one regulatory molecules that mediate maternal effects on embryonic development during preimplantation period. Our objective was to verify CSF2 blastocyst yield, determine posttransfer survival, and evaluate properties formed after treatment. vitro, increased percentage oocytes became morulae blastocysts. Blastocysts were treated with tended have a greater number inner cell mass cells had higher ratio trophectoderm...
Successful embryonic development is dependent on factors secreted by the reproductive tract. Dickkopf-1 (DKK1), an antagonist of wingless-related mouse mammary tumor virus (WNT) signaling pathway, one endometrial secretory protein potentially involved in maternal-embryo communication. The purpose this study was to investigate roles DKK1 embryo cell fate decisions and competence establish pregnancy. Using vitro-produced bovine embryos, we demonstrate that exposure embryos during period morula...
Choline is a vital micronutrient that can be utilized in the formation of betaine and multiple phospholipids. In this study, we aimed to confirm, expand on previous findings, how choline impacts embryos from first 7 days development affect postnatal phenotype. Bos indicus were cultured choline-free medium (termed vehicle) or supplemented with 1.8 mM Blastocyst-stage transferred into crossbred recipients. Once born, calves evaluated at birth, 94 d, 178 d weaning (average age = 239 d)....
Abstract Global warming is a major challenge to the sustainable and humane production of food because increased risk livestock heat stress. Here, example prolactin receptor ( PRLR ) gene used demonstrate how editing can increase resistance cattle stress by introduction mutations conferring thermotolerance. Several populations in South Central America possess natural that result affected animals having short hair being thermotolerant. CRISPR/Cas9 technology was introduce variants two...
An experiment was conducted to determine whether pregnancy rates following the transfer of in vitro-produced embryos heat-stressed cows could be improved by 1) culturing presence IGF-I and 2) treating recipients with GnRH. Lactating Holstein (n = 260) were synchronized using a timed ovulation protocol. Embryos produced vitro cultured or without 100 ng/mL IGF-I. On d 7 after anticipated (d 0), single embryo transferred all palpable corpus luteum 210). A subset 164) injected either GnRH...
Structural features of the dairy industry make it well situated to use embryo technologies as tools for enhancing genetic merit cattle and improving fertility. Technologies dependent upon transfer have potential increase efficiency quantitative selection marker-assisted selection, simplify cross-breeding germplasm conservation procedures allow incorporation transgenes into cattle. In addition, may prove useful in fertility infertile populations lactating cows. The realisation promise has...
Abstract Addition of insulin‐like growth factor‐1 (IGF‐1) to culture medium increases the proportion bovine embryos that develop blastocyst stage and embryo survival following transfer heat‐stressed, lactating dairy cows. The objective present study was determine molecular cellular correlates these actions IGF‐1. Embryos were produced in vitro cultured for 7 days with or without 100 ng/ml On d after insemination, grade 1 expanded blastocysts harvested used total cell number, percent...
Abstract Background While initially sensitive to heat shock, the bovine embryo gains thermal resistance as it progresses through development so that physiological shock has little effect on blastocyst stage by Day 5 after insemination. Here, experiments using 3’ tag digital gene expression (3’DGE) and real-time PCR were conducted determine changes in transcriptome of morula-stage embryos response (40 degrees C for 8 h) could be associated with thermotolerance. Results Using 3’DGE, 173 genes...
Exposure of bovine conceptuses to colony-stimulating factor 2 (CSF2) from days 5 7 development can increase the percentage transferred that develop term. The purpose this experiment was understand mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro presence or absence 10 ng/ml after insemination, into cows, flushed uterus at day 15 pregnancy. There a tendency (P=0.07) for proportion cows with recovered conceptus be greater those receiving...
Physiology of the adult can be modified by alterations in prenatal development driven maternal environment. Developmental programming, which established before embryo implants uterus, affect females differently than males. The mechanism sex-specific developmental programming is not known. Here we present evidence that regulatory signals change female embryos male embryos. In particular, actions maternally derived cytokine CSF2 from Day 5 to 7 affected characteristics at 15 for decreased...
Metritis is associated with reduced fertility in dairy cows, but the mechanisms are unclear because disease resolves several weeks before insemination. One hypothesis that metritis causes persistent changes granulosa cells during follicle development, which might be evident transcriptome of from dominant follicles after parturition. To test this hypothesis, we collected follicular fluid and 63 days post partum cows previously diagnosed metritis, at least 6 resolution not (control cows)....
Abstract Uterine infection is associated with infertility in women and dairy cows, even after the resolution of infection. However, mechanisms causing this persistent are unclear. Here, we hypothesized that induced endometritis non-lactating cows would reduce developmental competence oocytes. Non-lactating Holstein received an intrauterine infusion endometrial pathogenic bacteria (Escherichia coli Trueperella pyogenes; n = 12) or vehicle control (n 11) on day 2 estrous cycle. Bacterial...
Infection of the postpartum uterus with pathogenic bacteria is associated infertility months later in dairy cattle. However, it unclear whether these bacterial infections lead to long-term changes reproductive tract that might help explain this infertility. Here we tested hypothesis infusion into leads transcriptome 3 later. We used virgin Holstein heifers avoid potential confounding effects periparturient problems, lactation, and negative energy balance. Animals were infused intrauterine...