- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Ruminant Nutrition and Digestive Physiology
- Reproductive Biology and Fertility
- Effects of Environmental Stressors on Livestock
- Extracellular vesicles in disease
- Crop Yield and Soil Fertility
- MicroRNA in disease regulation
- Animal Nutrition and Health
- Cancer-related molecular mechanisms research
- Folate and B Vitamins Research
- Animal Nutrition and Physiology
- Birth, Development, and Health
- Modular Robots and Swarm Intelligence
- Milk Quality and Mastitis in Dairy Cows
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Indigenous Studies and Ecology
- Rabbits: Nutrition, Reproduction, Health
- Nuts composition and effects
- Dietary Effects on Health
- Ovarian function and disorders
- Animal Diversity and Health Studies
- Distributed systems and fault tolerance
- Agroforestry and silvopastoral systems
Southwest Florida Research
2023-2025
University of Florida
2021-2025
Ave Maria University
2022-2023
University of North Florida
2021
Choline is a vital micronutrient that can be utilized in the formation of betaine and multiple phospholipids. In this study, we aimed to confirm, expand on previous findings, how choline impacts embryos from first 7 days development affect postnatal phenotype. Bos indicus were cultured choline-free medium (termed vehicle) or supplemented with 1.8 mM Blastocyst-stage transferred into crossbred recipients. Once born, calves evaluated at birth, 94 d, 178 d weaning (average age = 239 d)....
Abstract Residual feed intake (RFI) is a widely employed metric to estimate efficiency, which compares actual expected intake. Enhancing efficiency while maintaining reproductive performance critical maximizing the profitability of beef production systems. Published data exhibit conflicting results concerning impact RFI on parameters. Our objective was evaluate relationship between and parameters in young Brangus bulls. Sixty bulls were randomly selected at beginning 56-day test. At start,...
This publication is intended for producers interested in improving the reproductive efficiency and performance of their cows. explains process estrus synchronization protocols that can be used natural service synchronization. Written by Daniella Heredia, Angela Gonella-Diaza, Kalyn Waters, published UF/IFAS Department Animal Sciences, February 2025.
Supplementation of choline chloride in culture medium programs the preimplantation bovine embryo to increase weaning weight resultant calf. Here, it was hypothesized that similar programming actions can be induced by feeding rumen-protected (RPC) beef cows during periconceptional period. A preliminary experiment conducted determine changes circulating concentrations and its metabolites after RPC supplementation. Suckled were individually fed 0, 30, 60, 90 g (i.e., 8.6, 17.3 25.9 chloride)...
Among the various seasonal environmental changes, elevated ambient temperature during summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role intrafollicular cellular communication by, part, mediating deleterious effects heat (HS). Here we aimed investigate changes FF-EV miRNA cargoes cows response changes: (SUM) compared winter (WIN) using high throughput...
Abstract Eighty-four Angus crossbred heifers (13 ± 1 mo of age, 329.5 61.92 kg body weight [BW]) were used in a generalized randomized block design with 2 × factorial arrangement treatments. The factors evaluated were: 1) diet type (whole plant sorghum silage [SS] vs. byproducts-based [BP]), and 2) feed additive: Aspergillus oryzae prebiotic (AOP; g/d) Negative control (CTL; 0 g/d), resulting four treatments: silage-control (SC), silage-AOP (SA), byproducts-control (BC), byproducts-AOP (BA)....
Abstract Changing climatic conditions are imposing risks and diminishing yields in agriculture. Sorghum (Sorghum bicolor) silage is a feasible option for backgrounding beef cattle terms of economic risk management animal productivity when compared with corn (Zea mays) silage, due to its drought adaptability. Similarly, Brassica carinata meal has proven be viable alternative as protein supplement forage-based systems, included at 10% the diet dry matter (DM). However, research scarce...
Abstract Two experiments were conducted to evaluate the effect of nonprotein nitrogen (NPN) supplementation on in vitro fermentation and animal performance using a backgrounding diet. In experiment 1, incubations three separate days (replicates). Treatments control (CTL, without NPN), urea (U), urea–biuret (UB), urea–biuret–nitrate (UBN) mixtures. Except for control, treatments isonitrogenous 1% U inclusion as reference. Ruminal fluid was collected from two Angus-crossbred steers fed diet...
Examining the mechanistic cellular responses to heat stress could aid in addressing increasing prevalence of decreased fertility due elevated ambient temperatures. Here, we aimed study differential oocytes and granulosa cells thermal fluctuations seasonal differences. Dry beef cows (n = 10) were housed together, synchronized subjected a stimulation protocol induce follicular growth before ovum pick-up (OPU). Two OPU's conducted (summer winter) collect cumulus-oocyte-complexes (COCs) cells....
Abstract Changes in maternal nutrition during the periconceptional period can influence postnatal growth cattle. This study aimed to identify impact of supplementing beef cows with rumen-protected methionine (RP-Met) on their female progeny. In exp 1, plasma (Met) levels were analyzed samples from 10 Angus crossbred, non-lactating cows. Cows randomly assigned receive 454 g cottonseed meal 15 g/d RP-Met (RPM; Smartamine M, Adisseo) or not (CON) for 5 d and data as a completely randomized...
Abstract One mechanism by which the maternal environment regulates early embryo is secretion of cell-signaling molecules. these dickkopf WNT signaling pathway inhibitor 1. Objectives were to (A) resolve discrepancies in literature regarding effects 1 bovine on development trophectoderm and competence establish pregnancy after transfer (B) determine whether there are long-term consequences placental function postnatal phenotype. Embryos produced vitro cultured with vehicle or 100 ng/mL...
Abstract Residual feed intake (RFI) is a measure of efficiency (FE). Differences in plasma metabolites concentration found animals with contrasting RFI are potential biomarkers FE. Furthermore, behavior patterns could also be used to identify RFI. We hypothesize that the integration metabolomics data and can predict future animal performance. 302 bulls subjected an ad libitum diet for 56-d while allocated pens equipped GrowSafe System evaluate Blood samples were collected analysis at...
Abstract This study aimed to identify the impact of supplementing rumen-protected methionine (RPM) during periconceptional period on beef cows in their progeny. Evidence suggests that changes maternal nutrition have effects postnatal growth phase cattle. We hypothesized feeding RPM would program bovine gestation enhance progeny growth. A total 114 were fed a roughage-based diet and randomized receive corn gluten supplemented with 15 g (Smartamine M, Adisseo) or not (CON) from d -7 +7...
Abstract Enhancement of feed efficiency (FE) along with reproductive performance is critical to maximize profitability beef production systems. Residual intake (RFI) a measure FE, assessing differences between expected and actual intake. Published data suggest negative impact RFI on performance. The objective this experiment was establish the relationship parameters related puberty in young bulls. Growing, Brangus bulls (n = 58; 10 12 mo age) were tested for FE over 56 d ranked as low (LRFI)...
Abstract This study aimed to evaluate embryo yield in Holstein heifers superovulated with a single injection of recombinant, long-acting human follicle-stimulating hormone (rFSH) vs. multiple injections pituitary-derived (FSH). In experiment 1, were assigned randomly one four experimental groups: Control (280 mg FSH; six 40 and two 20 mg, each ~12 h apart, n = 16); rFSH1 (50 µg FSH analog protein, rFSH2 (75 or rFSH3 (100 16). The rFSH was administered as injection. Estrous cycles...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Abstract Eighty-four Angus crossbred heifers (13 ± 1 mo, 329.5 61.92 kg of BW) were used in a generalized randomized block design with 2 × factorial arrangement treatments. The objective was to evaluate the effect feed additive inclusion under two growing diets. Factors included: 1) diet type (whole plant sorghum silage- or byproducts-based diet) and 2) [Amaferm (Aspergillus oryzae (extract)] included at 0 0.02% DM. This resulted four treatments: sorghum-control (SC), sorghum-amaferm (SA),...
Abstract Background Elevated ambient temperature during the summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role intrafollicular cellular communication by part mediating deleterious effects heat (HS). Here we aimed investigate changes FF-EV miRNA cargoes cows response HS (SUM) compared winter (WIN) using high throughput sequencing FF-EV-coupled miRNAs. In...
The objective of this study was to evaluate the effect a low dose hCG or FSH on follicle growth and pregnancy per artificial insemination (AI) in crossbreed beef heifers subjected split-time AI program. All (n = 386) were 7-d Co-synch protocol an intravaginal controlled internal drug release (CIDR) device (GnRH CIDR insertion; 7 days later PGF2α, removal, application tail head estrus detection patch; 36 hours detected estrus, 24 timed remaining heifers). At randomly assigned receive either...
Abstract During cell death, DNA is fragmented and reaches the bloodstream in form of cell‐free (cfDNA). Luteal cells must undergo an apoptotic process during structural luteolysis to begin a new oestrous cycle. We hypothesized that cfDNA concentrations would increase when inducing by applying prostaglandin F 2α (PGF2α) analog cycling cow. Multiparous non‐pregnant non‐lactating Angus cows ( Bos taurus ; n = 15) were synchronized using 7‐day CoSynch + CIDR protocol. Ten days after oestrus was...