A5022031145- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Effects of Environmental Stressors on Livestock
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Endometriosis Research and Treatment
- Animal Behavior and Welfare Studies
- Ruminant Nutrition and Digestive Physiology
- Sperm and Testicular Function
- Meat and Animal Product Quality
- Neuroendocrine regulation and behavior
- Animal Nutrition and Physiology
- Immune Response and Inflammation
- Pesticide Exposure and Toxicity
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Pharmacogenetics and Drug Metabolism
- Milk Quality and Mastitis in Dairy Cows
- Ovarian function and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- T-cell and Retrovirus Studies
- Environmental Toxicology and Ecotoxicology
- Animal Genetics and Reproduction
- Synthesis and Catalytic Reactions
- Birth, Development, and Health
University of Florida
2019-2024
National Institute of Environmental Health Sciences
2022-2023
Iowa State University
2016-2019
University of Maryland, College Park
2015
Abstract Uterine infection is associated with infertility in women and dairy cows, even after the resolution of infection. However, mechanisms causing this persistent are unclear. Here, we hypothesized that induced endometritis non-lactating cows would reduce developmental competence oocytes. Non-lactating Holstein received an intrauterine infusion endometrial pathogenic bacteria (Escherichia coli Trueperella pyogenes; n = 12) or vehicle control (n 11) on day 2 estrous cycle. Bacterial...
Study objectives were to determine the effects of zinc (Zn) amino acid complex (Availa Zn, Zinpro Corporation, Eden Prairie, MN) on metabolism, biomarkers leaky gut, and inflammation during following heat stress (HS) nutrient restriction. Crossbred gilts (n = 50; 50 ± 2 kg BW) blocked by initial BW randomly assigned one five treatments: 1) thermoneutral (TN) ad libitum fed a control diet (TNCtl), 2) TN pair-fed (PFCtl), 3) Zn-supplemented (PFZn), 4) HS (HSCtl), 5) (HSZn). The study consisted...
Environmental conditions that impede heat dissipation and increase body temperature cause stress (HS). The study objective was to evaluate impacts of HS on the follicular phase estrous cycle. Postpubertal gilts (126.0 ± 21.6 kg) were orally administered altrenogest synchronize estrus, subjected either 5 d thermal-neutral (TN; 20.3 0.5 °C; n = 6) or cyclical (25.4 - 31.9 during preceding behavioral estrus. On 5, blood samples obtained, euthanized, ovaries collected. Fluid from dominant...
Pregnancy induces changes in the transcriptome of bovine endometrium from 15 days after insemination. However, pregnancy is less likely to occur if cows had a postpartum bacterial infection uterus, even resolution disease. We hypothesized that uterine alters endometrial transcriptomic signature To examine pregnancy, were inseminated 130 intrauterine infusion pathogenic Escherichia coli and Trueperella pyogenes , subsequently was collected 16 insemination for RNA sequencing. found 171...
Mechanisms underlying obesity-associated reproductive impairment are ill defined. Hyperinsulinemia is a metabolic perturbation often observed in obese subjects. Insulin activates phosphatidylinositol 3-kinase (PI3K) signaling, which regulates ovarian folliculogenesis, steroidogenesis, and xenobiotic metabolism. The impact of progressive obesity on genes encoding mRNA involved insulin-mediated PI3K signaling biotransformation [insulin receptor (Insr), insulin substrate 1 (Irs1), 2 (Irs2), 3...
Abstract Genetically engineered mice are widely used to study the impact of altered gene expression in vivo. Within reproductive tract, Amhr2-IRES-Cre(Bhr) mouse model is ablate genes ovarian granulosa and uterine stromal cells. There reports inducing recombination non-target tissues. We hypothesized inefficiency or off-target Cre action due lack every cell that expresses Amhr2. To investigate, we created a new targeted knock-in model, Amhr2-iCre(Fjd), by inserting codon-optimized improved...
Diseases impacting the female reproductive tract pose a critical health concern. The establishment of in vitro models to study primary endometrial cells is crucial understanding mechanisms that contribute normal function and origins diseases. Established protocols for stromal cell culture have been use decades but recent advances organoid paved way allowing roles both epithelial vitro. Due inter-individual variability, cultures must be established from numerous persons. Generally, can...
Abstract Endometrial inflammation is associated with reduced pregnancy per artificial insemination (AI) and increased loss in cows. It was hypothesized that induced endometritis alters histotroph composition induces inflammatory signatures on conceptus compromise development. In Experiment 1, lactating cows were assigned to control (CON; n = 23) or an intrauterine infusion of Escherichia coli Trueperella pyogenes (ENDO; 34) induce endometritis. Cows received AI 26 days after treatment, the...
In brief Paternal high-gain diet reduces blastocyst development following in vitro fertilization and embryo culture but does not affect gene expression or cellular allocation of resultant blastocysts. Abstract Bulls used cattle production are often overfed to induce rapid growth, early puberty, increase sale price. While the negative consequences undernutrition on bull sperm quality known, it is unclear how a influences development. We hypothesized that semen collected from bulls fed would...
Summary An estimated 75% of unsuccessful pregnancies are due to implantation failure. Investigating the causes failure is difficult as decidualization and embryo a dynamic process. Here, we describe new decidua‐specific iCre recombinase mouse strain. Utilizing CRISPR/Cas9‐based genome editing, strain was developed that expresses under control endogenous prolactin family 8, subfamily a, member 2 (Prl8a2) promoter. activity examined by crossing with mTmG/+ or Sun1‐GFP reporter alleles....
Abstract Background Bacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation bacterial lipopolysaccharide (LPS) follicular fluid. Although treating granulosa cells with LPS vitro decreases expression synthesis enzyme CYP19A1 secretion, molecular mechanisms are unclear. The transcription factor CCAAT enhancer binding protein beta (CEBPβ) not only facilitates regulated cytokines, but also binds...
Abstract An estimated 75% of unsuccessful pregnancies are due to implantation failure. Investigating the causes failure is difficult as decidualization and embryo a dynamic process. Here we describe new decidua specific iCre recombinase mouse strain. Utilizing CRISPR/Cas9-based genome editing, strain was developed that expresses under control endogenous prolactin family 8, subfamily a, member 2 (Prl8a2) promoter. activity examined by crossing with mTmG or Sun1-GFP reporter alleles. initiated...
Heat stress (HS) results from an imbalance of thermal energy, and economic losses due to HS cost the U.S. swine industry approximately $900 million annually. negatively influences a variety parameters affecting reproduction: spontaneous abortion, longer wean-to-estrus interval, delayed puberty, reduced litter size, total number born, all which culminate in seasonal infertility. Additionally, compromises intestinal integrity ultimately leading metabolic endotoxemia (ME) increased systemic...
Heat stress (HS) is associated with decreased fertility and endotoxemia as evidenced by increased systemic lipopolysaccharide (LPS) arising from intestinal integrity. Across multiple species, LPS reduced female fecundity; phenotypic responses similar to HS-induced infertility, including spontaneous abortion wean-to-estrus interval length. binds toll-like receptor 4 (TLR4), a membrane bound receptor, initiate signaling cascade culminating in the phosphorylation of nuclear factor kappa-B...