A5017608631- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Carbohydrate Chemistry and Synthesis
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Cytokine Signaling Pathways and Interactions
- Vector-Borne Animal Diseases
- Genetic and phenotypic traits in livestock
- Chemical Reaction Mechanisms
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Chemical Synthesis and Analysis
- Reproductive Biology and Fertility
- Biochemical and Molecular Research
- Metabolism, Diabetes, and Cancer
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Viral gastroenteritis research and epidemiology
- Polyamine Metabolism and Applications
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Birth, Development, and Health
Colorado State University
2016-2025
Vrije Universiteit Amsterdam
2019-2025
Leiden University
2015-2024
University of Wisconsin–Milwaukee
2024
Universitat de Barcelona
2021-2022
Instituto de Química Orgánica General
2022
Zero to Three
2021
The University of Sydney
2016
University of Nebraska Medical Center
2012
Brazilian Agricultural Research Corporation
2012
Early gestation is critical for placentomal growth, differentiation, and vascularization, as well fetal organogenesis. The origins of adult disease hypothesis proposes that alterations in nutrition endocrine status result developmental adaptations permanently change structure, physiology, metabolism, thereby predisposing individuals to cardiovascular, metabolic, life. Multiparous ewes were fed 50% (nutrient restricted) or 100% (control fed) total digestible nutrients from Days 28 78...
The trophoblastic interferons ovine and bovine trophoblast protein-1 (oTP-1 bTP-1, respectively) have been implicated as mediators of maternal recognition pregnancy in sheep cattle. objective this study was to describe the onset duration gene expression for oTP-1 bTP-1 preimplantation conceptuses by situ hybridization Northern analysis. Sections from paraffin-embedded conceptuses, collected on Days 10, 11, 12, 13, 15 gestation (n = 1, 3, 2, 2), 12/13, 15/16, 19 4, 5), were hybridized...
The ruminant conceptus synthesizes and secretes interferon (IFN)-τ, which presumably acts via an intrauterine paracrine mechanism to signal maternal recognition of pregnancy. aims this study were determine whether IFN-stimulated genes (ISG) such as ISG15 OAS-1 are differentially expressed in blood cells circulating the uterus ewes; extrauterine components reproductive tract corpus luteum (CL) also express mRNA for these ISG, antiviral activity is greater uterine vein than artery during early...
Bovine trophoblast protein-1 (bTP-1) is a secreted glycoprotein that consists of several forms differing slightly in mol wt and isoelectric point. It produced by bovine conceptuses after about day 15 pregnancy believed to play key role signalling the presence an embryo mother. In this study, series recombinant cDNA clones corresponding mRNA for bTP-1 have been isolated from libraries representing 18–19 conceptus poly(A)+ mRNA. Base sequencing cDNAs indicated multiple mRNAs exist. Northern...
The acceptor dependence on the glycosylation stereoselectivity is revealed by a systematic study employing model acceptors of gradually changing nucleophilicity.
Interferon tau (IFNT) from the ovine conceptus has paracrine actions on endometrium that alter release of prostaglandin F(2alpha) (PGF) and protect corpus luteum (CL). Antiviral activity in uterine vein blood expression interferon-stimulated genes (ISGs) CL is greater pregnant than nonpregnant ewes. We hypothesized IFNT contributes to antiviral endocrine CL. Preadsorption with antiserum against recombinant (ro) revealed ewes was mediated by IFNT. Endocrine were examined after infusing either...
In cattle, the blastocyst hatches from zona pellucida on days 8-9 and then forms a conceptus that grows elongates into an ovoid filamentous shape between 9 16. The growing synthesizes secretes prostaglandins (PGs) interferon τ (IFNT). Our hypothesis was exerts local effect endometrium prior to maternal recognition of pregnancy day 16 in cattle. study one, synchronized cyclic heifers received no blastocysts or 20 vitro-produced 7 their uteri were collected 13. IFNT not detected by RIA uterine...
Abstract Controlling the chemical glycosylation reaction remains major challenge in synthesis of oligosaccharides. Though 1,2- trans glycosidic linkages can be installed using neighboring group participation, construction cis is difficult and has no general solution. Long-range participation (LRP) by distal acyl groups may steer stereoselectivity, but contradictory results have been reported on role strength this stereoelectronic effect. It exceedingly to study bridging dioxolenium ion...
The broad application of well-defined synthetic oligosaccharides in glycobiology and glycobiotechnology is largely hampered by the lack sufficient amounts carbohydrate specimens. Insufficient knowledge glycosylation reaction mechanism thwarts routine assembly these materials. Glycosyl cations are key reactive intermediates reaction, but their high reactivity fleeting nature have precluded determination clear structure-reactivity-stereoselectivity principles for species. We report a combined...
The α-effect is a term used to explain the dramatically enhanced reactivity of α-nucleophiles (R-Y-X:
Minimal structural differences in the structure of glycosyl donors can have a tremendous impact on their reactivity and stereochemical outcome glycosylation reactions. Here, we used combination systematic reactions, characterization potential reactive intermediates, in-depth computational studies to study disparate behavior systems involving benzylidene glucosyl mannosyl donors. While these been studied extensively, no satisfactory explanations are available for observed between 3-
ISG15 is induced by conceptus-derived interferon-τ in the endometrium on days 15–45 of pregnancy. It was hypothesized that pregnancy induces blood cell gene expression and low mRNA levels provide an indication non-pregnant cows day 18. Blood collected either 18 ( n = 78) or 15–21, 25, 32 21; serial collection) from dairy following artificial insemination (AI). Plasma progesterone concentration determined using RIA. were real-time PCR. Pregnancy diagnosed transrectal ultrasound. increased...
Ubiquitin cross-reactive protein (UCRP) is a 17-kDa that shows cross-reactivity with ubiquitin antisera and retains the carboxyl-terminal Leu-Arg-Gly-Gly amino acid sequence of ligates to, directs degradation of, cytosolic proteins. It has been reported bovine endometrial UCRP synthesized secreted in response to conceptus-derived interferon-tau (IFNtau). In present studies, mRNA were detected ovine endometrium. Ovine was detectable on Day 13, peaked at 15, remained high through 19 pregnancy....
A 16-kDa protein has been identified that is secreted by the bovine endometrium in response to conceptus-derived interferon (IFN)-τ during early pregnancy. Because this uterine was similar size a human ubiquitin cross-reactive (hUCRP) also regulated IFN, we suspected they might be related. To test hypothesis, flushings, medium from cultured endometrium, and endometrial tissues were examined for presence of ubiquitin-immunoreactive proteins. Immunoreacting proteins detected through use...
Journal Article Nucleotide and amino acid sequence of pap‐gene (pediocin AcH production) in Pediococcus acidilactici H Get access A.M. Motlagh, Motlagh Department Animal Science, University Wyoming, Laramie, WY 82071 Search for other works by this author on: Oxford Academic Google Scholar A.K. Bhunia, Bhunia †Department Food Science Biotechnology Center, Arkansas, Fayetteville, AR 72703, USA F. Szostek, Szostek T.R. Hansen, Hansen M.C. Johnson, Johnson B. Ray Letters Applied Microbiology,...
Ovine trophoblast protein-1 (oTP-1), the major product secreted by trophectoderm of sheep conceptus between days 13 and 21 pregnancy, is considered to mediate maternal recognition pregnancy maintaining function corpus luteum. Its amino acid sequence has 40–55% identity with various mammalian interferons-α (IFN-α), it been shown have antiviral activity. The present results confirm that oTP-1, which at 15–17 produced a single embryo more than 100 μg (>1 million units) per day, functional IFN....
Paracrine release of ovine interferon tau (oIFNT) from the conceptus alters endometrial prostaglandin F2 alpha (PGF) and prevents luteolysis. Endocrine oIFNT into uterine vein occurs by Day 15 pregnancy may impart resistance corpus luteum (CL) to PGF. It was hypothesized that infusion recombinant (roIFNT) or jugular veins on 10 estrous cycle would protect CL against exogenous PGF-induced Osmotic pumps were surgically installed in 24 ewes deliver bovine serum albumin (BSA; n = 12) roIFNT (200...
Arginine, the common substrate for production of nitric oxide (NO) and polyamines in mammals, increases uterine lumen during peri-implantation period pregnancy. However, functional roles arginine within conceptus (embryo extraembryonic membranes) development have not been elucidated vivo. To assess reproductive tissue survival conceptus, we conducted an vivo morpholino antisense oligonucleotide (MAO)-mediated knockdown SLC7A1 mRNA, transporter ovine trophectoderm (Tr). Translational mRNA...
Here, we have characterized 3-cyclopropyl-1-(4-(6-((1,1-dioxidothiomorpholino)methyl)-5-fluoropyridin-2-yl)benzyl)imidazolidine-2,4-dione hydrochloride (LEI-101) as a novel, peripherally restricted cannabinoid CB2 receptor agonist, using both in vitro and vivo models.