A5102021516- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Trace Elements in Health
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
- Neurological Disease Mechanisms and Treatments
- RNA Research and Splicing
- Electrochemical Analysis and Applications
- Animal Genetics and Reproduction
- Glycosylation and Glycoproteins Research
California Institute of Technology
2023
University of Florida
2019
Second Affiliated Hospital of Shantou University Medical College
2013
Shantou University
2013
Chinese Center For Disease Control and Prevention
2012
Hubei University
2005
Abstract Background HIV-1 p24 antigen is a major viral component of human immunodeficiency virus type 1 (HIV-1) which can be used to identify persons in the early stage infection and transmission from infected mothers infants. The detection usually accomplished by using an enzyme-linked immunosorbent assay (ELISA) with low sensitivity. Here we report use two bio-barcode amplification (BCA) assays combined polymerase chain reaction (PCR) gel electrophoresis quantify antigen. Method A pair...
The post-translational modification (PTM) of proteins by O-linked β-N-acetyl-D-glucosamine (O-GlcNAcylation) is widespread across the proteome during lifespan all multicellular organisms. However, nearly functional studies have focused on individual protein modifications, overlooking multitude simultaneous O-GlcNAcylation events that work together to coordinate cellular activities. Here, we describe Networking Interactors and SubstratEs (NISE), a novel, systems-level approach rapidly...
Objective To establish a novel assay for HIV-1 p24 ultrasensitive detection based on Gold Nanoparticle Probe (GNP) and PCR. Methods Sandwich ELISA method was established by pair of anti-p24 monoclonal antibodies (mAbs), 1G12 1D4, used to detect recombinant antigen.The bio-barcode DNA 47 bp, selected from genome Arabidopsis, formed double-stranded hybridization with the capture (complementary DNA) modified sulfhydryl.Then were conjugated surface 1D4-modified gold nanoparticles...
This protocol for using PCR to sex bovine embryos are based on Y-specific and autosomal targets described by Park et al, Theriogenology 2001; 55: 1843-1853 but with updates according current genomic database in NCBI as of 2019.Note that assembly the Y chromosome is still progress.
This protocol allows gene expression analysis within single embryos on multiple targets in either conventional real-time PCR or using the Fluidigm platform. The makes extensive use of CellsDirect Kit from ThermoFisher Scientific (catalog number 11753). References to kit refer this product. describes a method that converts RNA directly into cDNA without extraction, which avoids variation due extraction process. genes interest can be up more than 100. However, target for have determined prior...