Alexander J. McKeeken
A5036699245- PARP inhibition in cancer therapy
- RNA Research and Splicing
- Species Distribution and Climate Change
- Environmental DNA in Biodiversity Studies
- Data Analysis with R
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Genomics and Phylogenetic Studies
- HIV/AIDS drug development and treatment
- Acute Myeloid Leukemia Research
University of Minnesota
2023
University of Idaho
2020-2022
Abstract Deep learning is driving recent advances behind many everyday technologies, including speech and image recognition, natural language processing autonomous driving. It also gaining popularity in biology, where it has been used for automated species identification, environmental monitoring, ecological modelling, behavioural studies, DNA sequencing population genetics phylogenetics, among other applications. relies on artificial neural networks predictive modelling excels at...
Abstract RNA splicing factor (SF) gene mutations are commonly observed in patients with myeloid malignancies. Here we showed that SRSF2- and U2AF1-mutant leukemias preferentially sensitive to PARP inhibitors (PARPi), despite being proficient homologous recombination repair. Instead, SF-mutant exhibited R-loop accumulation elicited an R-loop–associated PARP1 response, rendering cells dependent on activity for survival. Consequently, PARPi induced DNA damage cell death R-loop-dependent manner....
Deep learning is driving recent advances behind many everyday technologies, including those relying on speech and image recognition, natural language processing, autonomous driving. It also gaining popularity in biology, where it has been used for automated species identification, environmental monitoring, behavioral studies, DNA sequencing, population genetics phylogenetics, among other applications. relies artificial neural networks predictive modeling excels at recognizing complex...
Antifolate resistance is significant in Kenya and presumed to result from extensive use cross-resistance between antifolate antimalarials antibiotics, including cotrimoxazole/Bactrim used for HIV-1 chemotherapy. However, little known about antifolate-resistant malaria the context of newly diagnosed co-infection prior administration Blood samples a cross-sectional study asymptomatic adult Kenyans enrolled during voluntary HIV testing were analyzed by PCR Plasmodium spp. More than 95%...
<p>Differential gene expression of K562 U2AF1-WT and U2AF1-S34F cells</p>
<p>BROCA-GO sequencing analysis</p>
<p>Alternative splicing analysis of K562 SRSF2-WT and SRSF2-P95H cells</p>
<p>GSEA analysis for Homologous repair related gene set in K562 SRSF2-P95H and U2AF1-S34F cells</p>
<p>List of plasmids</p>
<p>Primary human AML patient sample information</p>
<p>Primary human AML patient sample information</p>
<p>List of antibodies</p>
<p>BROCA-GO sequencing analysis</p>
<p>List of primers and sequences</p>
<p>Supplementary Figures S1-8</p>
<p>Alternative splicing analysis of K562 U2AF1-WT and U2AF1-S34F cells</p>
<div>Abstract<p>RNA splicing factor (SF) gene mutations are commonly observed in patients with myeloid malignancies. Here we showed that <i>SRSF2</i>- and <i>U2AF1</i>-mutant leukemias preferentially sensitive to PARP inhibitors (PARPi), despite being proficient homologous recombination repair. Instead, SF-mutant exhibited R-loop accumulation elicited an R-loop–associated PARP1 response, rendering cells dependent on activity for survival. Consequently,...
<p>GSEA analysis for Homologous repair related gene set in K562 SRSF2-P95H and U2AF1-S34F cells</p>
<p>List of inhibitors for drug screen and IC50 summary</p>
<p>Differential gene expression of K562 U2AF1-WT and U2AF1-S34F cells</p>
<div>Abstract<p>RNA splicing factor (SF) gene mutations are commonly observed in patients with myeloid malignancies. Here we showed that <i>SRSF2</i>- and <i>U2AF1</i>-mutant leukemias preferentially sensitive to PARP inhibitors (PARPi), despite being proficient homologous recombination repair. Instead, SF-mutant exhibited R-loop accumulation elicited an R-loop–associated PARP1 response, rendering cells dependent on activity for survival. Consequently,...
<p>List of antibodies</p>
<p>Supplementary Figures S1-8</p>
<p>List of primers and sequences</p>
<p>List of plasmids</p>