Urinary tract infection (UTI) is one of the most frequent infectious diseases and can occur in all age groups. Escherichia coli main cause this infection. Multiple resistances to antimicrobial agents are increasing quickly E. isolates may complicate therapeutic strategies for UTI. The aim study was determine antibiotic resistance pattern multidrug-resistance (MDR) phenotypes uropathogenic (UPEC).

Helicobacter pylori (H. pylori) is a spiral Gram negative bacteria that can transform to the coccoid form in adverse conditions.The aim of this study was determine vitro morphological and bactericidal effects metronidazole, amoxicillin clarithromycin on H. pylori.The standard strain 26695 cultured Brucella agar (BA) minimum inhibitory concentrations (MICs) three antibiotics were determined by E-test method. The exposed at 1/2 MIC, MIC 2X broth (BB). Induced forms confirmed staining light...

The role of integrons in the transfer antibiotic resistance is one important issues, therefore, this study aimed to investigate pattern and prevalence class 1 2 P. aeruginosa isolated. Out 72 confirmed isolates, 50% were from ICU patients. Antibacterial susceptibility showed that isolates most resistant ceftazidime (76.4%) colistin was effective (100%) molecular analysis I II 55.5% 29.1% positive, respectively proportions MDR significantly higher among integron-positive with 73.6% compared...

Abstract New Delhi metallo-β-lactamase variants and different types of metallo-β-lactamases have attracted enormous consideration for hydrolyzing almost all β-lactam antibiotics, which leads to multi drug resistance bacteria. Metallo-β-lactamases genes disseminated in hospitals parts the world became a public health concern. There is no inhibitor metallo-β-lactamase-1 other classes, so drugs an urgent need. In this study, multi-steps virtual screening was done over NPASS database with 35,032...

This study aimed to determine the prevalence of class I, II, and III integrons among clinical Acinetobacter baumannii isolates collected from hospitalized patients.This cross-sectional was conducted at two teaching hospitals in Isfahan, Iran, October 2015 2016. A total 147 non-duplicate A. were specimens identified as using standard microbiological methods confirmed by genotyping. Antimicrobial susceptibility determined disc diffusion method, presence integron genes performed polymerase...

Background: Helicobacter pylori (H. pylori) is one of the most common infectious diseases in world. It colonizes about 50-60% world's population. The estimated prevalence H. infection approximately 65% Iran. Objectives: aim present study was to investigate antimicrobial resistance metronidazole, clarithromycin and amoxicillin. Materials Methods: A total 110 biopsy specimens were collected from patients with clinical symptoms gastrointestinal disorders who referred endoscopy unit at Al-Zahra...

Background: Multidrug resistance Pseudomonas aeruginosa (MDR-P. aeruginosa) is a worldwide threat for public health. Hyperexpression of efflux pump systems (MexAB-OprM and MexCD-OprJ), which well-known mechanisms MDR emerging, controlled by regulatory genes, mexR nfxB, respectively. The aim this study was to evaluate point mutations in nfxB genes MDR- P. isolated from wound infections. Materials Methods: A total 34 cultures obtained infections were analyzed. Among them eight isolates...

Background: Extended-spectrum β-lactamase (ESBL)-producing is a significant resistant mechanism to β-lactams in Enterobacteriaceae, especially Klebsiella pneumoniae. The main objectives of this study were genetically characterize urinary clinical isolates K. pneumoniae through the investigating blaTEM, blaCTX-M and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined frequency antibiotic resistance strains...

Background: Acinetobacter baumannii is capable of forming biofilms that may be responsible for the survival this pathogen in hospital environment as well antibiotic resistance. Objectives: In study, considering importance genes bap, blaPER-1, and csuE formation resistance to antimicrobial drugs, we aimed investigate frequency these also relationship between biofilm formation. Methods: One hundred eighteen clinical strains A. were collected identified using standard microbiological methods....

Pseudomonas aeruginosa is a well-known opportunistic pathogen, which affects hospitalized patients in different wards due to its natural resistance drugs.The purpose of the current study was determine antibiotic susceptibility profiles and genetic relatedness P. isolated from admitted referral hospital Isfahan, Iran.Out 150 analyzed samples, 54 isolates were recovered subjected patterns diversity determination by Kirby-Bauer's disk diffusion method RAPD-PCR, respectively.The highest...

The aim of this study was to investigate the prevalence carbapenem-resistant Klebsiella pneumoniae (CRKP) and most common types carbapenemases, metallo-beta-lactamases (MBLs), extended-spectrum beta-lactamases (ESBLs) among CRKP isolates in a tertiary hospital Isfahan, Iran. Eighty non-repetitive clinical K. were obtained from different specimens. Antibiotic resistance pattern determined by disk diffusion method production carbapenemases MBLs confirmed using modified Hodge test E-test,...

Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence point mutations in clarithromycin-resistant clinical isolates H. Isfahan city Iran.Thirty from 130 biopsy specimens were isolated by culture and confirmed biochemical PCR tests. MIC clarithromycin antibiotic 30 determined E-test method. 288 bp 23S rRNA gene investigated four followed sequencing.Among pylori, 4 cases resistant to...

Early childhood caries is a kind of occurring in deciduous teeth. Bacteria are among the main factors. Antibacterial agents such as fluoride used both prevention and treatment, but their application children faces limitations fluorosis. Therefore, novel methods mainly focused on use fluoride-free active ingredients. In this comparative study, antibacterial effects gels containing propolis aloe vera, fluoride, xylitol, CPP-ACP were investigated.This an vitro study. By plate well technique,...

The objective of this study was to assess the prevalence, antibiogram, and related genes carbapenem-resistant Klebsiella pneumoniae (CRKP) among hospital environment samples. A total 250 samples were taken from different surfaces medical devices three hospitals in Isfahan, Iran. All cultured K. strains identified by conventional microbiological methods polymerase chain reaction (PCR). Antibiogram isolates performed disk diffusion method production carbapenemases metallo-β-lactamases (MBLs)...

The emergence of Metallo-beta-lactamase (MBL)-producing Acinetobacter baumannii has become a global concern in nosocomial infections. aim this study is to determine the prevalence MBL producing genes among clinical isolates A. from hospitalized patients.This was performed October 2015 2016 at three teaching hospitals located Isfahan, Iran. Totally, 100 A-baumannii were collected specimens and identified as using standard microbiological methods. Antimicrobial susceptibility test determined...

The present research decided to study prevalence of Porphyromonas gingivalis and Bacteroides forsythus in chronic periodontitis patient by use Multiplex PCR. subgingival plaque samples from 61 patients suffering with probing depth PD > or = 6 40 healthy controls were collected sterile curette. In this we used two species-specific Forward primers combination a single Reverse primer. These target variable conserved region 16S rRNA gene, respectively. included (34 women, 27 men; 24-69 years...