Cell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective this study was to investigate migratory response MSCs developed cobalt-incorporated hydroxyapatite (HACo) material. HACo fabricated by simple ion exchange procedure at concentrations ranging from 40 8000 μM into disc shape. discs were incubated in media conditioned (CM; HACoCM) collected culture. HACM served...

J Oral Pathol Med (2012) 41 : 713–720 Background: Zoledronic acid (ZA) is prescribed to treat various metabolic bone diseases. Despite its efficacy in preventing loss, ZA has been linked osteonecrosis of the jaw several reports. However, a mechanism underlying this occurrence still unclear. Objective: This study was investigate causative roles on osseous cellular activities pre‐osteoblastic cell line MC3T3‐E1 (MC3T3) and mesenchymal stem (MSC). Methods: Morphological analysis, RT‐PCR,...

A serious adverse effect of long-term bisphosphonate (BP) administration is bisphosphonate-related osteonecrosis the jaw (BRONJ). Among different proposed pathogenesis, suppression immune cells gaining interest. Because monocytes/macrophages could get access to BP since residing in blood and bone microenvironment, aim this study was analyze behaviors macrophages after treatments vitro.THP-1 cell, an established human monocytic cell model, used study. The effects BPs, alendronate (ALN)...

The global population is growing older and entering an aging society. Aging results in severe tissue disorder organ dysfunction. Bone-related injuries are particularly significant. need for alternative bone replacement materials human implants has grown over the past few decades. Alginate potential use as a cell scaffold engineering due to its high bio-compatibility. To improve bioactivity of alginate scaffolds, zinc- strontium-containing sol-gel-derived bioactive glass nanoparticles...

Silver diamine nitrate (SDN) is expected to help control caries similar silver fluoride (SDF). The aim of this study was determine the mineral precipitation in demineralized dentin and cytotoxicity SDN SDF dental pulp cells. Demineralized specimens were prepared, SDF, SDN, or water (control) applied. then remineralized simulated body fluid for 2 weeks. examined using FTIR-ATR, SEM-EDX, synchrotron radiation X-ray tomographic microscopy (SRXTM). Additionally, human stem cells analyzed an MTT...

Bone tissue engineering offers the prospect of alternative therapies for clinically relevant skeletal defects. Poly(butylene succinate) (PBSu) is a biodegradable and biocompatible polyester which possesses some unfavorable biomaterial properties. In order to improve this limitation, we developed PBSu/hydroxyapatite (HA) PBSu/β-tricalcium phosphate (TCP) composites support growth osteogenic differentiation human mesenchymal stem cells (hMSCs). The results showed that phase separation...

The aim of this study was to prepare RMGICs for pulp protection that contain polyacids functionalized with methacrylate groups (CMs) enable light-activated polymerization without the need toxic 2-hydroxyethyl (HEMA) monomers. effects using CM liquids 0 or 5 wt% HEMA on physical/mechanical properties and cytotoxicity experimental were assessed. Spherical pre-reacted glass fillers (SPG) used as powder phase. prepared by mixing SPG liquid (0 HEMA, F1) CMH (5 F2). Commercial materials...

Abstract Although poly(butylene succinate)/β‐tricalcium phosphate (PBSu/TCP) composites are biocompatible and allow the growth osteogenic differentiation of stem cells, cell attachment adhesion to PBSu‐based substrates is often limited. To enhance proliferation, we used a sodium hydroxide (NaOH) hydrolysis technique generate different degree roughness on PBSu/TCP with PBSu:TCP ratios. The results showed that NaOH increased surface in concentration‐dependent manner. Substrates higher ratios...

What is known and objective Alendronate (ALN) used for the treatment of post-menopausal osteoporosis. By reducing bone turnover, it increases mineral density. However, recent reports suggest an increased risk atypical fractures after long-term ALN administration. Despite its well-known anti-osteoclastic activity, unclear whether also suppresses human mesenchymal stem cell (hMSC)-mediated osteogenesis, thus possibly resulting in fragility. We hypothesized that does this we look at vitro...

Background Dental pulp stem cells (DPSCs) possess mesenchymal cell characteristics and have potential for cell-based therapy. Cell expansion is essential to achieve sufficient numbers. However, continuous replication causes aging in vitro , which usually accompanies potentially affect DPSC activities. Continuous passaging could alter susceptibility external factors such as drug treatment. Therefore, this study sought investigate outcome of on morphology activities the absence or presence...

Bone engineering offers the prospect of alternative therapies for clinically relevant skeletal defects. Poly(butylene succinate) (PBSu) is a biodegradable and biocompatible polyester which may possess some limitations in clinical use due to its hydrophobicity. In order overcome these increase bioactivity, simple convenient surface hydrolysis PBSu, PBSu/hydroxyapatite PBSu/β-tricalcium phosphate (TCP) films was performed. The resulting surfaces (i.e., HPBSu, HPBSu/HA HPBSu/TCP) were tested...

The aim of this study was to assess the chemical/mechanical properties ion-releasing dental sealants containing strontium-bioactive glass nanoparticles (Sr-BGNPs) and monocalcium phosphate monohydrate (MCPM). Two experimental sealants, TS1 (10 wt% Sr-BGNPs 2 MCPM) TS2 (5 4 MCPM), were prepared. Commercial controls ClinproXT (CP) BeautiSealant (BT). monomer conversion (DC) tested using ATR–FTIR (n = 5). biaxial flexural strength (BFS) modulus (BFM) determined 5) following 24 h 7 days...

Bone substitute is a therapeutic approach to treat bone abnormalities. A scaffold serves mainly as osteoconductive elements. To facilitate better biological performance, short collagen peptide was immobilized onto hydrolyzed poly(butylene succinate)/β-tricalcium phosphate (HPBSu/TCP) scaffolds. PBSu/TCP (80:20) scaffolds were fabricated by supercritical CO2 technique, with 0.6 M NaOH and conjugated tagged or without red fluorescence. The surface morphology porous structure of characterized...

Dental caries is a major oral health problem, which associates with cariogenic bacteria. Streptococcus mutans and Lactobacillus acidophilus are facultative anaerobic bacteria that found in tooth decay. Accordingly, neem leaf extract was developed due to its great anti-microbial property against many The aim of this study determine anti-cariogenic properties novel paste preparation.The derived from maceration dry leaves ethanol for 48 h. ethanolic subjected chemical identification using...

Abstract Background Alendronate (ALN) is a bisphosphonate, which prescribed as an anti‐osteoporotic drug. ALN has been shown to increase osteoblast cell death and decrease bone mineralization. inhibits key regulatory enzyme in the mevalonate pathway, consequently reducing geranylgeranyl pyrophosphate (GGPP). Geranylgeraniol (GGOH) can be converted GGPP. The aim of this study was investigate effects exogenous GGOH on MC3T3 viability, cycle, function, cytoskeleton under treatment. Methods...

Although attempts have been made to immobilize dual short peptides on a biomaterial surface, the optimization, characterization and functional analysis of peptide immobilization onto poly(buthylene succinate)/β-tricalcium phosphate (PBSu/TCP) composites not yet reported. The present study was, therefore, carried out optimize characterize type I collagen (COLsp) bone morphogenetic protein-2 (BMP-2sp) hydrolyzed PBSu/TCP (HPBSu/TCP) composites, bioactivity resulting peptide-immobilized...