A5015414210- Anaerobic Digestion and Biogas Production
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Wastewater Treatment and Nitrogen Removal
- Catalysts for Methane Reforming
- Microbial Fuel Cells and Bioremediation
- Carbon Dioxide Capture Technologies
- Microbial Community Ecology and Physiology
- Hydrocarbon exploration and reservoir analysis
- Microbial bioremediation and biosurfactants
- Microbial Metabolites in Food Biotechnology
- Microbial metabolism and enzyme function
- Digestive system and related health
- Atmospheric and Environmental Gas Dynamics
- Food composition and properties
- Probiotics and Fermented Foods
- Methane Hydrates and Related Phenomena
- Electrocatalysts for Energy Conversion
- Enzyme Production and Characterization
- Wildlife Ecology and Conservation
- Ecology and Vegetation Dynamics Studies
- Reservoir Engineering and Simulation Methods
- Environmental and Social Impact Assessments
- Water-Energy-Food Nexus Studies
- Genomics and Phylogenetic Studies
University of Minho
2014-2024
Universidade do Porto
2024
Instituto Superior Técnico
2023
Cooperativa de Ensino Superior Politécnico e Universitário
2022
Ministério da Saúde e da Segurança Social
2022
Wageningen University & Research
2008-2016
Universidade do Estado de Santa Catarina
2015
Universidade Federal do Rio Grande do Sul
2014
Institute for Biotechnology and Bioengineering
2008-2013
Mineralization of a synthetic effluent containing 50% COD as oleic acid was achieved in continuous anaerobic reactor at organic loading rates up to 21 kg m−3 day−1, HRT 9 h, attaining 99% removal efficiency and methane yield higher than 70%. A maximum specific production rate 1170 ± 170 mg COD-CH4 g VS−1 day−1 measured during the reactor's operation. start-up strategy combining feeding phases batch degradation applied promote development an community efficient for long chain fatty acids...
A novel anaerobic, thermophilic, carbon monoxide-utilizing bacterium, strain E3-O T , was isolated from anaerobic sludge a municipal solid waste digester. Cells were straight rods, 0.6–1 µm in diameter and 2–3 length grew as single cells or pairs. formed round terminal endospores. The temperature range for growth 50–70 °C, with an optimum at 65 °C. pH 5.7–8.0, 7.5. Strain had the ability to ferment various sugars, such fructose, galactose, glucose, mannose, raffinose, ribose, sucrose xylose,...
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by use ultrasound technology or its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated synthesis butyl acetate using MCI-CALB under ultrasonic energy, comparing results against those obtained commercial preparation, Novozym 435. optimal conditions were determined response surface methodology (RSM) evaluating...
Thermophilic (55 °C) anaerobic microbial communities were enriched with a synthetic syngas mixture (composed of CO, H2 , and CO2 ) or CO alone. Cultures T-Syn T-CO incubated successively transferred (16 transfers) (9 transfers), respectively, increasing partial pressures from 0.09 to 0.88 bar. Culture T-Syn, after 4 successive transfers syngas, was also subsequently solely this substrate - cultures T-Syn-CO. Incubation caused rapid decrease in the diversity consortium. T-Syn-CO showed...
Syngas is a substrate for the anaerobic bioproduction of fuels and valuable chemicals. In this study, sludge was used microbial enrichments with synthetic syngas acetate as main substrates. The objectives study were to identify networks (in enrichment cultures) conversion added-value products, isolate robust, non-fastidious carboxydotrophs. Enrichment cultures produced methane propionate, last one an unusual product from fermentation. A bacterium closely related Acetobacterium wieringae...
Syngas fermentation has great potential for the sustainable production of chemicals from wastes (via prior gasification) and flue gases containing CO/CO 2 . Research efforts need to be directed toward expanding product portfolio gas fermentation, which is currently limited mainly acetate ethanol.
Anaerobic bacteria involved in the degradation of long-chain fatty acids (LCFA), presence sulfate as electron acceptor, were studied by combined cultivation-dependent and molecular techniques. The bacterial diversity four mesophilic sulfate-reducing enrichment cultures, growing on oleate (C(18:1), unsaturated LCFA) or palmitate (C(16:0), saturated LCFA), was denaturing gradient gel electrophoresis (DGGE) profiling polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments. These...
Developing new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, feedstock usually represents highest cost, which becomes target for cost reduction. Additionally, biorefinery concept advocates revenue growth from several compounds using same feedstock. Taken together, bio commodities low-cost gas streams containing CO, CO2, H2, obtained gasification any...
Desulfotomaculum nigrificans and D. carboxydivorans are moderately thermophilic members of the polyphyletic spore-forming genus in family Peptococcaceae. They phylogenetically very closely related belong to 'subgroup a' cluster 1. have a similar growth substrate spectrum; they can grow with glucose fructose as electron donors presence sulfate. Additionally, both species able ferment fructose, although fermentation is only reported for carboxydivorans. 20% carbon monoxide (CO) coupled sulfate...
Microbial conversion of carbon monoxide (CO)/syngas has been extensively investigated. The microbial CO/syngas offers numerous advantages over chemically catalyzed processes e.g. the specificity biocatalysts, operation at ambient conditions and high efficiencies. Bioelectrochemical systems (BESs) exploit capacity electrochemically active bacteria (EAB) to use insoluble electron acceptors or donors produce electricity added-value compounds. Electricity production from different organic...
Epilactose is a disaccharide composed of galactose and mannose, it currently considered an “under development” prebiotic. In this study, we described the prebiotic potential epilactose by in vitro fermentation using human fecal inocula from individuals following Mediterranean diet (DM) or Vegan (DV). The effect was also compared with lactulose raffinose, interesting correlations were established between metabolites microbiota modulation. production several (lactate, short-chain fatty acids,...
Treatment of anaerobic granules with heat and two chemical treatments, contacting 2-bromoethanesulfonate (BES) BES + Chloroform, were applied to suppress hydrogen-consuming microorganisms. Three mesophilic expanded granular sludge bed (EGSB) reactors-R(Heat), R(BES), R(BES Chlo)--were inoculated the treated sludges fed synthetic sugar-based wastewater (5 g(COD) L(-1), HRT 20-12 h). Morphological integrity bacterial communities assessed by quantitative image analysis 16S rRNA gene based...
Summary The substitution of natural gas by renewable biomethane is an interesting option to reduce global carbon footprint. Syngas fermentation has potential in this context, as a diverse range low‐biodegradable materials that can be used. In study, anaerobic sludge acclimatized syngas multi‐orifice baffled bioreactor ( MOBB ) was used start enrichments with CO . main goals were identify the key players conversion and evaluate interspecies metabolic interactions conferring robustness...
Microbial communities with the ability to convert long-chain fatty acids (LCFA) coupled sulfate reduction can be important in removal of these compounds from wastewater. In this work, an enrichment culture, able oxidize acid palmitate (C 16 : 0 ) reduction, was obtained anaerobic granular sludge. Microscopic analysis designated HP revealed that it mainly composed one morphotype a typical collar-like cell wall invagination, distinct morphological feature Desulfomonile genus. 16S rRNA gene...
A syngas-degrading enrichment culture, culture T-Syn, was dominated by a bacterium closely related to Desulfofundulus australicus strain AB33T (98 % 16S rRNA gene sequence identity). Culture T-Syn could convert high CO concentrations (from pCO ≈ 34 kPa 170 kPa), both in the absence and presence of sulfate as external electron acceptor. The products formed from conversion were H2 acetate. With sulfate, lower H2/acetate ratio observed profile, but rates similar those sulfate. ability D. use...
Abstract Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from same inoculum (anaerobic granular sludge), with high specialization, within phyla ( Thermodesulfobacteriota , Euryarchaeota Pseudomonadota ). The are...
Summary 1‐Hexadecene‐contaminated wastewater is produced in oil refineries and can be treated methanogenic bioreactors, although generally at low conversion rates. In this study, a microbial culture able to degrade 1‐hexadecene was enriched, different stimulation strategies were tested for enhancing methane. Seven three times faster methane production obtained cultures stimulated with yeast extract or lactate, respectively, while amended crotonate lost the ability 1‐hexadecene. Methane from...
An anaerobic thermophilic strain (strain PCO) was isolated from a syngas-converting enrichment culture. Syngas components cannot be used by PCO, but the new is very tolerant to carbon monoxide (pCO = 1.7 × 10(5) Pa, 100% CO). 16S rRNA gene analysis and DNA-DNA hybridization revealed that PCO of Thermoanaerobacter thermohydrosulfuricus. The physiology other species compared, focusing on their tolerance monoxide. T. thermohydrosulfuricus, brockii subsp. finnii, pseudethanolicus, wiegelii were...