Summary Carbon materials have been reported to facilitate direct interspecies electron transfer (DIET) between bacteria and methanogens improving methane production in anaerobic processes. In this work, the effect of increasing concentrations carbon nanotubes (CNT) on activity pure cultures typical fatty acid‐degrading syntrophic methanogenic coculture was evaluated. CNT affected by cultures, although acceleration higher for hydrogenotrophic than acetoclastic or coculture. Interestingly,...

Polyhydroxyalkanoates (PHAs) are important candidates for replacing petroleum-based plastics. This transition is urgent the development of a biobased economy and to protect human health natural ecosystems. PHAs biodegradable polyesters that when blended with other polymers, such as poly(butylene adipate-co-terephthalate) (PBAT), acquire remarkable improvements in their properties, which allow them comply requirements packaging applications. However, biodegradation blends should be tested...

Lipids can be anaerobically digested to methane, but methanogens are often considered highly sensitive the long-chain fatty acids (LCFA) deriving from lipids hydrolysis. In this study, effect of unsaturated (oleate [C18:1]) and saturated (stearate [C18:0] palmitate [C16:0]) LCFA toward methanogenic archaea was studied in batch enrichments pure cultures. Overall, oleate had a more stringent on than LCFA, degree tolerance different among distinct species methanogens. Methanobacterium...

Mineralization of a synthetic effluent containing 50% COD as oleic acid was achieved in continuous anaerobic reactor at organic loading rates up to 21 kg m−3 day−1, HRT 9 h, attaining 99% removal efficiency and methane yield higher than 70%. A maximum specific production rate 1170 ± 170 mg COD-CH4 g VS−1 day−1 measured during the reactor's operation. start-up strategy combining feeding phases batch degradation applied promote development an community efficient for long chain fatty acids...

Long-chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures Methanosaeta concilii Methanosarcina mazei was assessed comparing rates from acetate before after LCFA addition. For both methanogens, a sharp decrease in (> 50%) observed at 0.5 mmol L(-1) oleate, no formed concentrations higher than 2 oleate. Palmitate less inhibitory M. more tolerant to mazei, with causing 11% 64% inhibition respectively. This study...

Conversion of unsaturated long chain fatty acids (LCFA) to methane in continuous bioreactors is not fully understood. Palmitate (C16:0) often accumulates during oleate (C18:1) biodegradation methanogenic bioreactors, and the reason why this happens which microorganisms catalyze reaction remains unknown. Facultative anaerobic bacteria are frequently found reactors operated at high LCFA loads, but their function unclear. To get more insight on role these bacteria, conversion was studied under...

Omics and meta-omics technologies are powerful approaches to explore microorganisms' functions, but the sheer size complexity of omics datasets often turn analysis into a challenging task. Software developed for analyses, together with knowledgebases encompassing information on genes, proteins, taxonomic functional annotation, among other types information, valuable resources analyzing data. Although several bioinformatics available many require significant computational expertise. Web...

Abstract Plastic pollution poses a worldwide environmental challenge, affecting wildlife and human health. Assessing the biodegradation capabilities of natural microbiomes in environments contaminated with microplastics is crucial for mitigating effects plastic pollution. In this work, we evaluated potential landfill leachate (LL) estuarine sediments (ES) to biodegrade polyethylene (PE), terephthalate (PET), polycaprolactone (PCL), under aerobic, anaerobic, thermophilic, mesophilic...

The conversion of organic matter to methane through anaerobic digestion (AD) process can be enhanced by different materials. However, literature reports show inconsistent results on the effect materials in AD systems. In this study, we evaluated influence inoculum's activity production (MP) efficiency presence (activated carbon (AC), magnetite (Mag), and zeolite (Zeo)). inocula included pure cultures methanogens, syntrophic cocultures, complex microbial communities, kinetic parameters...

Degradation of long-chain fatty acids (LCFAs) in methanogenic environments is a syntrophic process involving the activity LCFA-degrading bacteria and hydrogen-utilizing methanogens. If methanogens are inhibited, other hydrogen scavengers needed to achieve complete LCFA degradation. In this work, we developed two different oleate (C18:1 LCFA)-degrading anaerobic enrichment cultures, one (ME) another which methanogenesis was inhibited (IE). Inhibition attained by adding solution...

In anaerobic bioreactors, the electrons produced during oxidation of organic matter can potentially be used for biological reduction pharmaceuticals in wastewaters. Common electron transfer limitations benefit from acceleration reactions through utilization redox mediators (RM). This work explores potential carbon nanomaterials (CNM) as RM on removal ciprofloxacin (CIP). Pristine and tailored nanotubes (CNT) were first tested chemical CIP, pristine CNT was found best material, so it further...

Abstract Thermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, Sargassum sp . biomass, is studied this work batch assays also continuous reactor experiment. Pursuing the interest studying interactions between inorganic materials (adsorbents, conductive others) anaerobic bacteria, biological processes were amended with variable amounts zeolite type-13X range zeolite/inoculum (in VS)...

Conductive materials (CM) enhance methanogenesis, but there is no clear correlation between conductivity and faster methane production (MP) rates. We investigated if MP by pure cultures of methanogens (Methanobacterium formicicum, Methanospirillum hungatei, Methanothrix harundinacea Methanosarcina barkeri) affected CM (activated carbon (AC), magnetite), other sustainable alternatives (sand glass beads, without conductivity, zeolites (Zeo)). The significant impact the was on M. formicicum as...

Microbial communities with the ability to convert long-chain fatty acids (LCFA) coupled sulfate reduction can be important in removal of these compounds from wastewater. In this work, an enrichment culture, able oxidize acid palmitate (C 16 : 0 ) reduction, was obtained anaerobic granular sludge. Microscopic analysis designated HP revealed that it mainly composed one morphotype a typical collar-like cell wall invagination, distinct morphological feature Desulfomonile genus. 16S rRNA gene...

Abstract The analysis of meta‐omics data requires the utilization several bioinformatics tools and proficiency in informatics. integration multiple is even more challenging, outputs existing solutions are not always easy to interpret. Here, we present a pipeline, Meta‐Omics Software for Community Analysis (MOSCA), which aims overcome these limitations. MOSCA was initially developed analysing metagenomics (MG) metatranscriptomics (MT) data. Now, it also performs MG metaproteomics (MP)...