A5036644249- Cardiovascular Disease and Adiposity
- IL-33, ST2, and ILC Pathways
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- Clinical Nutrition and Gastroenterology
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Diabetes and associated disorders
- Electrolyte and hormonal disorders
- Nutrition and Health in Aging
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Queen Mary University of London
2021-2024
William Harvey Research Institute
2021-2024
King's College London
2021-2023
Asthma UK
2021
Queen's Medical Centre
1983
BACKGROUND. Epicardial adipose tissue (EAT) directly overlies the myocardium, with changes in its morphology and volume associated myriad cardiovascular metabolic diseases. However, EAT's immune structure cellular characterization remain incompletely described. We aimed to define phenotype of EAT humans compare such profiles across lean, obese, diabetic patients.
Atrial fibrillation (AF) is the most common sustained arrhythmia and carries an increased risk of stroke heart failure. Here we investigated how immune infiltrate human epicardial adipose tissue (EAT), which directly overlies myocardium, contributes to AF. Flow cytometry analysis revealed enrichment tissue-resident memory T (T
Antibodies of the IgD isotype remain least well characterized mammalian immunoglobulin isotypes. Here we report three-dimensional structures for Fab region IgD, based on four different crystal structures, at resolutions 1.45–2.75 Å. These crystals provide first high-resolution views unique Cδ1 domain. Structural comparisons identify regions conformational diversity within domain, as among homologous domains Cα1, Cγ1 and Cμ1. The structure also possesses a conformation upper hinge region,...
This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human mouse species. A number of downstream applications can then be performed gain an appreciation the functional activity unique tissue-resident cell populations. For complete details on use execution this protocol, please refer Macdougall et al. (2018).
We have previously produced a toolkit of antibodies, comprising recombinant human antibodies all but one the isotypes, directed against polcalcin family antigen Phl p 7. In this work, we complete antibody isotypes with IgD version anti-Phl 7 monoclonal antibody. also raised set nanobodies and identify characterize paratope-specific nanobody. This nanobody binds to IgE isotype antibody, which shares same idiotype, orthosterically inhibits interaction The 2.1 Å resolution X-ray crystal...
In order to better understand how the immune system interacts with environmental triggers produce organ-specific disease, we here address hypothesis that B and plasma cells are free migrate through mucosal surfaces of upper lower respiratory tracts, their total antibody repertoire is modified in a common tract this case atopic asthma. Using Adaptive Immune Receptor Repertoire sequencing (AIRR-seq) have catalogued repertoires cell clones retrieved near contemporaneously from multiple sites...
<title>Abstract</title> Thymic involution is an age-related process that results in reduced T cell production and impaired adaptive immunity. However, it remains uncertain the extent of thymic activity later life how this contributes to immunological ageing. Here, we identified tissue within mediastinal adipose locations aged individuals confirmed its functional capacity through multi-omics analysis. Percentage CD31+ CD4+ cells correlated positively with presence. output elderly showed a...
<title>Abstract</title> Atrial fibrillation (AF) is the most common sustained arrhythmia and carries an increased risk of stroke heart failure. Here, we investigate how immune infiltrate human epicardial adipose tissue (EAT), which directly overlies myocardium, contributes to AF. Flow cytometry analysis revealed enrichment tissue-resident memory T (T<sub>RM</sub>) cells in AF patients. Cellular indexing transcriptomes epitopes by sequencing (CITE-Seq) single-cell TCR sequencing, identified...
<h3>Introduction</h3> Systemic markers of inflammation strongly correlate with an increased risk atrial fibrillation (AF). However, the local immune drivers this AF remain poorly defined. Recently, a wealth imaging data has established volume adipose tissue overlying heart, epicardial (EAT), as independent factor for all forms AF. profile EAT driving again remains undefined. Currently, human studies are sparse patients typically matched baseline clinical characteristics. This study sought to...