A5075322000- Microbial Metabolic Engineering and Bioproduction
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Circadian rhythm and melatonin
- Enzyme Catalysis and Immobilization
- Long-Term Effects of COVID-19
- Microbial Natural Products and Biosynthesis
- Fungal and yeast genetics research
- Genetic Associations and Epidemiology
- Diet, Metabolism, and Disease
- Spaceflight effects on biology
- Mass Spectrometry Techniques and Applications
- Biotin and Related Studies
- Diet and metabolism studies
- COVID-19 Clinical Research Studies
- Nutrition, Genetics, and Disease
- Pharmacology and Obesity Treatment
- Genetics, Bioinformatics, and Biomedical Research
- Gene Regulatory Network Analysis
- Dietary Effects on Health
- Thermoregulation and physiological responses
University of Toronto
2016-2022
Ted Rogers Centre for Heart Research
2020-2022
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and 350,000 positive interactions. This comprehensive maps interactions essential gene pairs, highlighting genes as densely connected hubs. Genetic profiles enabled assembly of hierarchical model cell function, including modules corresponding to protein complexes pathways, biological processes, cellular compartments. Negative...
Trigenic interactions in yeast link bioprocesses To dissect the genotype-phenotype landscape of a cell, it is necessary to understand between genes. Building on digenic protein-protein interaction network, Kuzmin et al. created trigenic by using synthetic genetic array (see Perspective Walhout). Triple-mutant analyses indicated that majority genes with associations functioned within same biological processes. These converged networks identified landscape. Although overall effects were weaker...
Abstract Aims Circadian rhythms orchestrate important functions in the cardiovascular system: contribution of microvascular to disease progression/severity is unknown. This study hypothesized that (i) myogenic reactivity skeletal muscle resistance arteries rhythmic and (ii) disrupting this rhythmicity would alter cardiac injury post-myocardial infarction (MI). Methods results Cremaster were isolated assessed using standard pressure myography. was globally disrupted with ClockΔ19/Δ19 mutation...
Our understanding of metabolic networks is incomplete, and new enzymatic activities await discovery in well-studied organisms. Mass spectrometric measurement cellular metabolites reveals compounds inside cells that are unexplained by current maps reactions, existing computational models unable to account for all observed within cells. Additional large-scale genetic biochemical approaches required elucidate gene function. We have used full-scan mass spectrometry metabolomics polar small...
Elevated total peripheral resistance (TPR) is a hallmark of many cardiovascular diseases and furthers disease progression. We previously demonstrated that myogenic tone, regulated by membrane-bound tumour necrosis factor (mTNF) reverse signalling, primary modulator TPR. Thus, inhibiting mTNF signalling should reduce TPR with the potential to improve outcome. However, since ubiquitous protein critical biological functions, its indiscriminate inhibition can cause widespread side effects....