A5087432027- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Proteoglycans and glycosaminoglycans research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Glaucoma and retinal disorders
- Advanced Drug Delivery Systems
- Corneal surgery and disorders
- Salivary Gland Disorders and Functions
- Organ Donation and Transplantation
- Ocular Infections and Treatments
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Pediatric health and respiratory diseases
- Connexins and lens biology
- Fibroblast Growth Factor Research
- Psoriasis: Treatment and Pathogenesis
- Ocular Diseases and Behçet’s Syndrome
- Biomedical Ethics and Regulation
- Organ and Tissue Transplantation Research
- Polysaccharides and Plant Cell Walls
- Hematopoietic Stem Cell Transplantation
- Barrier Structure and Function Studies
Dana-Farber Cancer Institute
2018-2024
CoImmune (United States)
2023
Boston University
2013-2021
Massachusetts Eye and Ear Infirmary
2012-2015
Harvard University
2013-2015
Universidad de Valladolid
2008-2014
Smith-Kettlewell Eye Research Institute
2013
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2011-2012
Universidade de Santiago de Compostela
2010
Ocular surface inflammation associated with Sjögren’s syndrome is characterized by a loss of secretory function and alteration in numbers mucin secreting goblet cells. Such changes are prominent feature ocular inflammatory diseases attributed to inflammation; however, the exact effect cytokines on conjunctival cell remains largely unknown. In this study, we developed primary culture mouse cells from tissue evaluated effects their detected conjunctiva model (Thrombospondin-1 deficient mice)....
Goblet cells are secretory epithelial of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion soluble mucins. Loss these is associated with several chronic inflammatory disorders the mucosa. Although demonstrated to transfer antigens luminal surface stromal in intestinal mucosa, it not known if goblet contribute regulation an immune response. In this study we report similar respiratory epithelia, mouse ocular epithelia predominantly...
Decreased production of the mucin MUC5AC in eye is related to several pathological conditions, including dry syndrome. A specific strategy for increasing ocular levels not yet available. Using a plasmid specially designed encode human MUC5AC, we evaluated ability hybrid cationized gelatin nanoparticles (NPs) containing polyanions (chondroitin sulfate or dextran sulfate) transfect epithelial cells. NPs were developed using ionic gelation technique and characterized by small size (<200 nm),...
Lacrimal gland inflammation during autoimmune Sjögren's syndrome (SS) leads to ocular surface – Keratoconjunctivitis sicca (KCS). This condition afflicts both the cornea and conjunctiva that form surface. Thrombospondin-1 (TSP-1) deficiency in mice results lacrimal corneal resembles human disease. In this study we report conjunctival pathology mouse model of SS. We found TSP-1 null develop associated loss goblet cell function similar seen patients with Increased expression Th1 (IFN-γ, TNF-α)...
Hyaluronic acid-chitosan nanoparticles (HA-CS NPs) have the potential to serve as a reliable drug delivery system topically treat ocular surface disorders. We evaluated in vivo uptake by structures, acute tolerance, and possible alterations of tear film physiology rabbits.Fluorescent HA-CS NPs (fl-HA-CS were prepared ionotropic gelation using fluoresceinamine-labeled hyaluronic acid resuspended buffer. fl-HA-CS (30 microL, 0.5 mg/mL) fluoresceinamine-HA conjugate microL) instilled into...
Purpose: The aim of the study was to determine effect inflammatory conditions on expression tight junction (TJ) and adherens (AJ) proteins between human corneal epithelial cells and, consequently, barrier integrity.Materials methods: Zonula occludens ZO-1 ZO-2, claudin-1 -2 (CLDN-1 CLDN-2), occludin (OCLN) as well E-cadherin (E-cad) were analyzed in a cell line (HCE) at basal after stimulation with cytokines (TNFα, TGFβ, IL-10, IL-13, IL-17, IL-6), using real time RT-PCR, Western blotting...
Chronic inflammation of the ocular surface poses a risk vision impairment. The understanding molecular mechanisms that are involved in inflammatory response is critical to identify novel targets. Recently, thrombospondin-1 (TSP-1) has emerged as key player homeostasis efficiently activates TGF-β2 isoform predominantly expressed mucosa. Here, potential peptide derived from TSP-1 (KRFK), can activate TGF-β, proposed potentially applicable therapeutic for chronic disorders. Our vitro results...
An important role of transforming growth factor-β (TGF-β) in the development regulatory T cells is well established. Although integrin-mediated activation latent TGF-β1 considered essential for induction (Treg) by antigen-presenting (APCs), such an mechanism not applicable to TGF-β2 isoform, which lacks integrin-binding RGD sequence its latency-associated peptide. Mucosal and ocular tissues harbour TGF-β2-expressing APCs involved Treg induction. The mechanisms that regulate TGF-β remain...
Purpose.: Toelucidate the role of trefoil family peptide (TFF) 3 at ocular surface under conditions similar to dry eye disease (DED) and in tears patients suffering from DED. Methods.: Trefoil levels tear samples non-Sjögren's DED with moderate were analyzed by ELISA compared healthy volunteers. A human corneal epithelial (HCE) cell line was treated proinflammatory cytokines IL-1β TNF-α, hyperosmolar medium, or scratching for up 24 hours. gene expression protein biosynthesis RT-PCR,...
Purpose: Increased expression of transforming growth factor-β2 (TGF-β2) is reported in the conjunctiva dry eye patients with no increase anti-inflammatory activity TGF-β2. Our aim was to compare molecules involved TGF-β2 activation, thrombospondin-1 (TSP-1) and CD36, during murine human conjunctival inflammation. Methods: Human tissue from cadaveric donors, epithelial primary cells fibroblasts, conjunctivas were immunostained for TSP-1, or Inflamed tissues obtained C57BL/6 wild-type (WT)...
Purpose: The potential role of commensals as triggering factors that promote inflammation in dry eye disease has not been explored. objective this study was to evaluate whether ocular microbiota changes with the onset thrombospondin-1-deficient (TSP-1−/−) mice, a strain develops Sjögren's syndrome-like disease. Methods: Conjunctival swabs were collected from TSP-1−/− and C57BL/6 mice analyzed for bacterial presence. Opsonophagocytosis conjunctival isolates derived aged by neutrophils either...
Conjunctival epithelium forms a barrier between the ocular surface microbial flora and mucosa. In addition to secreting gel-forming mucins, goblet cells, located in conjunctival epithelium, help maintain local immune homeostasis by active TGFβ2 promoting tolerogenic phenotype of dendritic cells vicinity. Although cell subsets, characteristic mucosal tissues, are found conjunctiva, previous studies provided limited information about their location within tissue. this study, we examine...
Anti-inflammatory agents are routinely used to treat inflammatory diseases affecting both anterior and/or posterior segments of the eye. Corticosteroids and NSAIDs can be administered either topically onto ocular surface structures or injected into However, one most important handicaps treatments is presence several anatomical physiological barriers in eye that drastically reduce drug access target site. The hydrophobicity anti-inflammatory agents, low bioavailability, invasiveness...