A5072702584- Enzyme Structure and Function
- Venomous Animal Envenomation and Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Enzyme Production and Characterization
- Computational Drug Discovery Methods
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Electrochemical sensors and biosensors
- Conducting polymers and applications
- Biochemical and Structural Characterization
- Insect and Pesticide Research
- Biofuel production and bioconversion
- Protein purification and stability
- Studies on Chitinases and Chitosanases
- interferon and immune responses
- Cardiac electrophysiology and arrhythmias
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Neuroscience and Neural Engineering
- Biotin and Related Studies
A N Bach Institute of Biochemistry
2012-2024
Russian Academy of Sciences
2009-2021
Federal Center Research Fundamentals of Biotechnology
2019-2021
Kurchatov Institute
2009-2018
Moscow Institute of Physics and Technology
2012
Centre National de la Recherche Scientifique
2004-2010
A.V. Shubnikov Institute of Crystallography
2010
Institut de Génétique Moléculaire de Montpellier
2010
Institute of Crystallography
2009
Institute of Bioorganic Chemistry
1992-2007
The isolation of the peptide inhibitor M-type K+ current, BeKm-1, from venom Central Asian scorpion Buthus eupeus has been described previously (Fillipov A. K., Kozlov, S. A., Pluzhnikov, K. Grishin, E. V., and Brown, D. (1996) FEBS Lett. 384, 277–280). Here we report cloning, expression, selectivity BeKm-1. A full-length cDNA 365 nucleotides encoding precursor BeKm-1 was isolated using rapid amplification ends polymerase chain reaction technique mRNA obtained telsons. Sequence analysis...
Human plasma butyrylcholinesterase (BChE) is an endogenous bioscavenger that hydrolyzes numerous medicamentous and poisonous esters scavenges potent organophosphorus nerve agents. BChE thus a marker for the diagnosis of OP poisoning. It also considered therapeutic target against Alzheimer's disease. Although X-ray structure partially deglycosylated monomer human was solved 15 years ago, all attempts to determine 3D natural full-length glycosylated tetrameric have been unsuccessful so far....
The scorpion toxin BeKm-1 is unique among a variety of known short toxins affecting potassium channels in its selective action on ether-a-go-go-related gene (ERG)-type channels. shares the common molecular scaffold with other toxins. spatial structure resolved by NMR consists α-helix and triple-stranded antiparallel β-sheet. By mutagenesis study we identified residues that are important for binding to human ERG K+ (HERG) channel. most critical (Tyr-11, Lys-18, Arg-20, Lys-23) located...
HU proteins belong to the nucleoid-associated (NAPs) that are involved in vital processes such as DNA compaction and reparation, gene transcription etc. No data available on structures of from mycoplasmas. To this end, protein parasitic mycoplasma Spiroplasma melliferum KC3 was cloned, overexpressed Escherichia coli purified homogeneity. Prismatic crystals were obtained by vapour-diffusion technique at 4°C. The diffracted 1.36 Å resolution (the best ever for a protein). diffraction indexed...
Neuronal apoptosis has been shown to require de novo RNA/protein synthesis. However, very few genes whose expression is necessary for inducing have identified so far. To systematically identify such genes, we used genome-scale, long oligonucleotide microarrays and characterized the gene profile of cerebellar granule neurons in early phase elicited by KCl deprivation. We 368 significantly differentially expressed including most previously reported be transcriptionally regulated this paradigm....
Abstract Novel disulfide-containing polypeptide toxin was discovered in the venom of Tibellus oblongus spider. We report on isolation, spatial structure determination and electrophysiological characterization this 41-residue toxin, called ω-Tbo-IT1. It has an insect-toxic effect with LD 50 19 μg/g experiments house fly Musca domestica larvae 20 juvenile Gromphadorhina portentosa cockroaches. Electrophysiological revealed a reversible inhibition evoked excitatory postsynaptic currents blow...
Abstract
Abstract Background the use of specific but partially degenerate primers for nucleic acid hybridisations and PCRs amplification known or unknown gene families was first reported well over a decade ago technique has been used widely since then. Results here we report novel successful selection strategy design hybrid with RT-PCR RACE-PCR identification families. The (named PaBaLiS) proven very effective as it allowed us to identify clone large group mRNAs encoding neurotoxin-like polypeptide...
We have developed and synthesized nanobiocomposite materials based on graphene, poly(3,4-ethylenedioxythiophene), glucose oxidase immobilized the surface of various nanomaterials (gold nanoparticles multi-walled carbon nanotubes) different sizes (carbon nanotubes diameters). Comparative studies possible influence nanomaterial's nature bioelectrocatalytic characteristics glucose- oxidizing bioanodes in a neutral phosphate buffer solution demonstrated that current densities nanocomposite-based...
This work aims to study molecular mechanisms involved in the formation of DNA-containing microparticles and nanoparticles during PCR. Both pyrophosphate Mg2+ ions proved play an important role generation DNA (MPs) with a unique sophisticated structure PCR Taq polymerase. Thus, addition Tli thermostable pyrophosphatase mixture inhibited this process caused destruction synthesized MPs. Thermal cycling Na-pyrophosphate (Na-PPi)- Mg2+-containing mixtures (without polymerase dNTPs) under standard...
The crystal structure of recombinant prolidase from Thermococcus sibiricus was determined by X-ray diffraction at a resolution 2.6 Å and found to contain tetramer in the asymmetric unit. A protein grown microgravity using counter-diffusion method used for studies. belonged space group P21221, with unit-cell parameters = 97.60, b 123.72, c 136.52 Å, α β γ 90°. refined an Rcryst 22.1% Rfree 29.6%. revealed flexible folding N-terminal domain as well high variability positions bound metal ions....