A5043238874- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Mast cells and histamine
- Insect Resistance and Genetics
- Cardiac electrophysiology and arrhythmias
- Pain Mechanisms and Treatments
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Insect and Pesticide Research
Institute of Evolutionary Physiology and Biochemistry
2014-2023
Abstract Novel disulfide-containing polypeptide toxin was discovered in the venom of Tibellus oblongus spider. We report on isolation, spatial structure determination and electrophysiological characterization this 41-residue toxin, called ω-Tbo-IT1. It has an insect-toxic effect with LD 50 19 μg/g experiments house fly Musca domestica larvae 20 juvenile Gromphadorhina portentosa cockroaches. Electrophysiological revealed a reversible inhibition evoked excitatory postsynaptic currents blow...
Acid-sensing ion channels (ASICs) are widely distributed in both the central and peripheral nervous systems of vertebrates. The pharmacology these receptors remains poorly investigated, while search for new ASIC modulators is very important. Recently, we found that some monoamines, which blockers NMDA receptors, inhibit and/or potentiate acid-sensing channels, depending on subunit composition channels. effect 9-aminoacridine, IEM-1921, IEM-2117, memantine native recombinant ASIC1a, ASIC2a,...
Acid-sensing ion channel 3 (ASIC3) is an important member of the acid-sensing channels family, which widely expressed in peripheral nervous system and contributes to pain sensation. ASICs are targeted by various drugs toxins. However, mechanisms structural determinants ligands’ action on ASIC3 not completely understood. In present work we studied modulation a series “hydrophobic monoamines” their guanidine analogs, were previously characterized affect other ASIC via multiple mechanisms....
The action of tetraalkylammonium ions, from tetrametylammonium (TMA) to tetrapentylammonium (TPtA), on the recombinant and native acid-sensing ion channels (ASICs) was studied using patch-clamp approach. responses ASIC1a, ASIC2a, heteromeric ASICs were inhibited by TPtA. peak currents through ASIC3 unaffected, whereas steady-state significantly potentiated. This effect characterized an EC
Abstract Among the proton-activated channels of ASIC family, ASIC1a exhibits a specific tachyphylaxis phenomenon in form progressive decrease response amplitude during series activations. This process is well known, but its mechanism poorly understood. Here, we demonstrated partial reversibility this effect by long-term whole-cell recording CHO cells transfected with rASIC1a cDNA. Long infrequent acidifications provided same recovery time course as short frequency. Steady-state...