A5049066124- Enzyme Structure and Function
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
- Porphyrin Metabolism and Disorders
- Glycosylation and Glycoproteins Research
- Crystallization and Solubility Studies
- HIV/AIDS drug development and treatment
- Receptor Mechanisms and Signaling
- vaccines and immunoinformatics approaches
- Neuropeptides and Animal Physiology
- Bacteriophages and microbial interactions
- Neurological diseases and metabolism
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- Photosynthetic Processes and Mechanisms
- Folate and B Vitamins Research
- Enzyme Production and Characterization
- Protease and Inhibitor Mechanisms
- Acute Lymphoblastic Leukemia research
- Cytomegalovirus and herpesvirus research
- Lipid Membrane Structure and Behavior
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Enzyme function and inhibition
Kurchatov Institute
2015-2024
A.V. Shubnikov Institute of Crystallography
2015-2024
Russian Academy of Sciences
2015-2024
Federal Scientific Research Centre Crystallography and Photonics
2016-2024
Institute of Crystallography
2024
Institute of Bioorganic Chemistry
2017-2024
Saint-Petersburg State University of Technology and Design
2024
Buryat State Academy of Agriculture
2020-2022
Saint Petersburg State University of Aerospace and Instrumentation
2020-2021
Agricultural Academy
2020
Mucormycosis is a potentially fatal illness that arises in immunocompromised people due to diabetic ketoacidosis, neutropenia, organ transplantation, and elevated serum levels of accessible iron. The sudden spread mucormycosis COVID-19 patients engendered massive concern worldwide. Comorbidities including diabetes, cancer, steroid-based medications, long-term ventilation, increased ferritin concentration trigger favorable fungi growth turn effectuate mucormycosis. necessity FTR1 gene-encoded...
From the base structure of tetragonal lysozyme crystals, octamer cluster was selected as possible element crystal growth. The proposed model verified by small-angle X-ray scattering (SAXS) measurements solutions. results showed a noticeable presence dimers and octamers under crystallization conditions total absence oligomers where growth impossible.
Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged same space group, I2(1)3, unit-cell parameters a = b c 77.365 A, alpha beta gamma 90.00 degrees. The reported group are almost identical those cubic in PDB. results X-ray studies confirmed that crystals obtained they contained no or its fragments. Electron-density maps calculated using diffraction sets from...
Proteins are the most important biological macromolecules, and involved in almost all aspects of life. Therefore, study structure proteins is great practical fundamental importance. On one hand, knowledge spatial necessary to basic principles protein functioning; for example, mechanisms enzymatic reactions. other used, biotechnology, design enzymes with desired properties, as well drug design. Today, main method determining a X-ray structural analysis crystals. The difficulty applying this...
Background The structure and function of bacterial nucleoid are controlled by histone-like proteins HU/IHF family, omnipresent in bacteria also founding archaea some eukaryotes.HU protein binds dsDNA without sequence specificity avidly DNA structures with propensity to be inclined such as forks, three/four-way junctions, nicks, overhangs bulges. Sequence comparison thousands known from diverse phyla reveals relation between sequence, DNA–binding properties other features. Methodology...
Lassa mammarenavirus (LASMV) is responsible for a specific type of acute viral hemorrhagic fever known as fever. Lack effective treatments and counter-measures against the virus has resulted in high mortality rate its endemic regions. Therefore, this study, novel epitope-based vaccine been designed using methods immunoinformatics targeting glycoprotein nucleoprotein virus. After numerous robust analyses, two CTL epitopes, eight HTL epitopes seven B-cell were finally selected constructing...
The development of technologies for the efficient synthesis innovative antiviral compounds remains an important challenge modern biotechnology, especially in context recent COVID-19 pandemic. One drugs that is currently research spotlight potential anti-SARS-CoV-2 activity purine mimetic prodrug compound T-705, also known as favipiravir. Along with a similar compound, T-1105, activation T-705 limited by low rate phosphoribosylation, mediated enzyme named hypoxanthine-guanine...
Structures of recombinant phosphopantetheine adenylyltransferase (PPAT) from Mycobacterium tuberculosis (PPATMt) in the apo form and complex with substrate ATP were determined at 1.62 1.70 Å resolution, respectively, using crystals grown microgravity by counter-diffusion method. The molecule PPATMt-ATP was located full occupancy active-site cavity. Comparison solved structures previously PPATMt complexed reaction product dephosphocoenzyme A (dPCoA) feedback inhibitor coenzyme (CoA) performed...
The structural study of complexes thymidine phosphorylase (TP) with nucleoside analogues which inhibit its activity is special interest because many these compounds are used as chemotherapeutic agents. Determination kinetic parameters showed that 3'-azido-3'-deoxythymidine (3'-azidothymidine; AZT), widely for the treatment human immunodeficiency virus, a reversible noncompetitive inhibitor Escherichia coli (TP). three-dimensional structure E. TP complexed AZT was solved by...
Communicated by Ramaswamy H. Sarma
The crystal structures of carboxypeptidase T (CpT) complexes with phenylalanine and arginine substrate analogs - benzylsuccinic acid (2-guanidinoethylmercapto)succinic were determined by the molecular replacement method at resolutions 1.57 Å 1.62 to clarify broad specificity profile enzyme. conservative Leu211 Leu254 residues (also present in both A B) shown be structural determinants for recognition hydrophobic substrates, whereas Asp263 was positively charged substrates. Mutations these...
Human cytomegalovirus (HCMV) is a widespread virus that can cause serious and irreversible neurological damage in newborns even death children who do not have the access to much-needed medications. While some vaccines drugs are found be effective against HCMV, their extended use has given rise dose-limiting toxicities development of drug-resistant mutants among patients. Despite half century's worth research, lack licensed HCMV vaccine heightens need develop newer antiviral therapies...
The pre-crystallization solution of the transaminase from Thermobaculum terrenum (TaTT) has been studied by small-angle X-ray scattering (SAXS). Regular changes in oligomeric composition protein were observed after addition precipitant. Comparison oligomers with crystal structure TaTT (PDB ID 6GKR) shows that dodecamers may act as building blocks growth single crystals. Correlating these results to similar studies other proteins suggests SAXS be a valuable tool for searching optimum...
Abstract Here we report bisphenol derivatives of fluorene (BDFs) as a new type chemical probes targeting histone-like HU protein, global regulator bacterial nucleoids, via its dimerization interface perturbation. BDFs were identified by virtual screening and molecular docking that targeted the core DNA-binding β-saddle-like domain protein from Spiroplasma melliferum. However, NMR spectroscopy, complemented with dynamics site-directed mutagenesis, indicated actual site inhibitors’...
Oligopeptidase B (OpB) is a two-domain, trypsin-like serine peptidase belonging to the S9 prolyloligopeptidase (POP) family. Two domains are linked by hinge region that participates in transition of enzyme between two major states—closed and open—in which residues catalytic triad located close each other separated, respectively. In this study, we described, for first time, structure OpB from bacteria obtained an Serratia proteomaculans with modified (PSPmod). PSPmod was crystallized...