A5085958894- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Eicosanoids and Hypertension Pharmacology
- Radiation Effects and Dosimetry
- Neurotransmitter Receptor Influence on Behavior
- Fluorine in Organic Chemistry
- GABA and Rice Research
- Biochemical Analysis and Sensing Techniques
- Nitric Oxide and Endothelin Effects
- Cholinesterase and Neurodegenerative Diseases
- Inflammatory mediators and NSAID effects
- Chemical Reactions and Isotopes
- Neuroscience of respiration and sleep
- Gyrotron and Vacuum Electronics Research
- Diet, Metabolism, and Disease
- Particle accelerators and beam dynamics
- Pulsed Power Technology Applications
- Neurological Disorders and Treatments
- Receptor Mechanisms and Signaling
- Synthesis and Biological Evaluation
- Marine Invertebrate Physiology and Ecology
- Environmental Toxicology and Ecotoxicology
- Pharmacological Receptor Mechanisms and Effects
- Fatty Acid Research and Health
Institute of Bioorganic Chemistry
2016-2025
Budker Institute of Nuclear Physics
2006-2025
Institute of Bioorganic Chemistry
2022-2024
S.P.E.C.I.E.S.
2022
Siberian Branch of the Russian Academy of Sciences
2006-2021
Kurchatov Institute
2021
Institute of Molecular Genetics
1988-2021
Novosibirsk State University
2017
Russian Academy of Sciences
2006-2015
Koltzov Institute of Developmental Biology
2001
We reported previously that synthetic amides of polyunsaturated fatty acids with bioactive amines can result in substances interact proteins the endogenous cannabinoid system (ECS). Here we synthesized a series N-acyl-dopamines (NADAs) and studied their effects on anandamide membrane transporter, amidohydrolase (fatty acid amide hydrolase, FAAH) two receptor subtypes, CB(1) CB(2). NADAs competitively inhibited FAAH from N18TG2 cells (IC(50)=19-100 microM), as well binding selective ligand,...
Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged same space group, I2(1)3, unit-cell parameters a = b c 77.365 A, alpha beta gamma 90.00 degrees. The reported group are almost identical those cubic in PDB. results X-ray studies confirmed that crystals obtained they contained no or its fragments. Electron-density maps calculated using diffraction sets from...
We reported previously that synthetic amides of polyunsaturated fatty acids with bioactive amines can result in substances interact proteins the endogenous cannabinoid system (ECS). Here we synthesized a series N-acyl-dopamines (NADAs) and studied their effects on anandamide membrane transporter, amidohydrolase (fatty acid amide hydrolase, FAAH) two receptor subtypes, CB1 CB2. NADAs competitively inhibited FAAH from N18TG2 cells (IC50 = 19–100µM), as well binding selective ligand,...
Relevance. The problem of prevention and treatment influenza remains relevant due to the consistently high incidence rate disease in population all age groups, especially children elderly. Antiviral drugs have become most important tools fight against viruses. main is search development new antiviral that do not side effects. aim present study investigate effect a synthetic drug candidate based on adamantane (NMG 1110) its combination with ribavirin reproduction A virus MDCK cell culture....
Chlorpyrifos targets mammalian brain development through a combination of effects directed at cholinergic receptors and intracellular signaling cascades that are involved in cell differentiation. We used sea urchin embryos as an invertebrate model system to explore the cellular mechanisms underlying actions chlorpyrifos delineate critical period developmental vulnerability. Sea larvae were exposed different stages ranging from early cleavages prism stage. Although unaffected even high...
Cholines acylated with unsaturated fatty acids are a recently discovered family of endogenous lipids. However, the data on biological activity acylcholines remain very limited. We hypothesized that containing residues arachidonic (AA-CHOL), oleic (Ol-CHOL), linoleic (Ln-CHOL), and docosahexaenoic (DHA-CHOL) act as modulators acetylcholine signaling system. In radioligand binding assay, showed inhibition in micromolar range both α7 neuronal nAChR overexpressed GH4C1 cells muscle type from...
Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While inhibits the of cells, LPI is known as stimulant. Despite endocannabinoid receptor crosstalk simultaneous presence microenvironment both molecules, their combined activity has never been studied. We evaluated effect on AEA six human breast lines different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin...
2-arachnadoyl glycerol (2-AG) is one of the most common endocannabinoid molecules with anti-proliferative, cytotoxic, and pro-proliferative effects on different types tumors. Typically, it induces cell death via cannabinoid receptor 1/2 (CB1/CB2)-linked ceramide production. In breast cancer, counterbalanced by sphingosine-1-phosphate, thus mechanisms 2-AG influence proliferation are poorly understood. We evaluated mechanism anti-proliferative action lysophaosphatidylinositol (LPI) in six...
Abstract β‐Lactoglobulin (β‐LG) is a lipocalin, which the major whey protein of cow's milk and other mammals. However, it absent from human milk. The biological function β‐LG not clear, but its potential role in carrying fatty acids through digestive tract has been suggested. found complexes with lipids such as butyric oleic high affinity for wide variety compounds. Serotonin (5‐hydroxytryptamine, 5‐HT), an important compound animals plants, various functions, including regulation mood,...
The strontium ferrite radiation-thermal synthesis process due to heating by intensive electron beam is described. and thermal processes kinetics were studied compared.
Abstract N ‐acyl dopamines (NADAs) are bioactive lipids of the endovanilloid family with known cytotoxicity for cancer cells; however, available data on participation endovanilloids in epithelial–mesenchymal transition (EMT) and stemness controversial. This study unveils inhibitory role ‐arachidonoyl dopamine (AA‐DA), a typical representative NADA family, breast cell migration, EMT, stemness. AA‐DA treatment also led to decrease cholesterol biosynthesis gene expressions, addition exogenous...
Despite the wide variety of available cationic lipid platforms for delivery nucleic acids into cells, optimization their composition has not lost its relevance. The purpose this work was to develop multi-component nanoparticles (LNPs) with or without a hydrophobic core from natural lipids in order evaluate efficiency LNPs widely used lipoid DOTAP (1,2-dioleoyloxy-3-[trimethylammonium]-propane) and previously unstudied oleoylcholine (Ol-Ch), as well ability containing GM3 gangliosides...
GPR55 is a non-canonical cannabinoid receptor, important for cancer proliferation. Depending on the ligand, it induces either cell proliferation or death. The objective of study was to establish mechanisms this multidirectional signaling. Using CRISPR-Cas9 system, GPR55, CB1, CB2, and GPR18 receptor knockouts MDA-MB-231 line were obtained. After CB2 knockout, pro-apoptotic activity ligand docosahexaenoyl dopamine (DHA-DA) slightly increased, while pro-proliferative most active synthetic...
Stabilized melanocortin analog peptide ACTH(6–9)PGP (HFRWPGP) possesses a wide range of neuroprotective activities. However, its mechanism action remains poorly understood. In this paper, we present study the proproliferative and cytoprotective activity adrenocorticotropic hormone fragment 6–9 (HFRW) linked with prolyine–glycyl–proline on SH-SY5Y cells in model oxidative stress-related toxicity. The dose-dependently protected from H2O2, tert-butyl hydroperoxide, KCN demonstrated activity....
Breast cancer stands as the leading cause of cancer-related deaths among women worldwide. Endocannabinoids and their exogenous analogs, e.g., tetrahydrocannabinol, exhibit antitumor effects in various animal models cancer. However, several studies have shown that under certain conditions, treatment with cannabinoids can stimulate proliferation cells vitro disrupt immune system’s involvement suppressing tumors. Additionally, conflicting reports exist regarding role endocannabinoid system This...
Embryos and larvae of sea urchins (Lytechinus variegatus, Strongylocentrotus droebachiensis, purpuratus, Dendraster excentricus), starfish (Pisaster ochraceus) were investigated for the presence a functional endocannabinoid system. Anandamide (arachidonoyl ethanolamide, AEA), was measured in early L. variegatus embryos by liquid chromatography/mass spectrometry. AEA showed strong developmental dynamic, increasing more than 5-fold between 8-16 cell mid-blastula 2 stage. 'Perturb-and-rescue'...