A5074184896- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Methane Hydrates and Related Phenomena
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Ionosphere and magnetosphere dynamics
- Historical and Environmental Studies
- Byzantine Studies and History
- Microbial infections and disease research
- Biochemical and Molecular Research
- Renaissance and Early Modern Studies
- Ancient Mediterranean Archaeology and History
- Historical Economic and Social Studies
- Complementary and Alternative Medicine Studies
- Italian Fascism and Post-war Society
- Leech Biology and Applications
- Healthcare and Venom Research
- Multiculturalism, Politics, Migration, Gender
- Neuropeptides and Animal Physiology
- Enzyme Structure and Function
- Phytoplasmas and Hemiptera pathogens
- Classical Antiquity Studies
Northwestern University
2021-2023
Moscow Institute of Physics and Technology
2016-2023
Science for Life Laboratory
2018
Karolinska Institutet
2018
Karolinska University Hospital
2018
Mediating transcription The Mediator complex is recruited by factors to all protein-coding genes in eukaryotes and helps assemble the machinery necessary transcribe gene. Abdella et al. present cryo–electron microscopy structure of human Mediator-bound preinitiation (Med-PIC). shows how positions long, flexible C-terminal domain RNA polymerase II be phosphorylated kinase CDK7, a crucial step for further processing into mature RNA. Most sites where bind are flexibly tethered complex, allowing...
Background The structure and function of bacterial nucleoid are controlled by histone-like proteins HU/IHF family, omnipresent in bacteria also founding archaea some eukaryotes.HU protein binds dsDNA without sequence specificity avidly DNA structures with propensity to be inclined such as forks, three/four-way junctions, nicks, overhangs bulges. Sequence comparison thousands known from diverse phyla reveals relation between sequence, DNA–binding properties other features. Methodology...
RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the rRNA promoter requires transcription factors TFIIIA, TFIIIC, TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) visualize S. cerevisiae complex of TFIIIA TFIIIC bound promoter. Gene-specific factor interacts with DNA acts as an adaptor TFIIIC-promoter interactions. We also binding TFIIIB subunits, Brf1 TBP (TATA-box protein), which results in...
Destabilase from the medical leech Hirudo medicinalis belongs to family of i-type lysozymes. It has two different enzymatic activities: microbial cell walls destruction (muramidase activity), and dissolution stabilized fibrin (isopeptidase activity). Both activities are known be inhibited by sodium chloride at near physiological concentrations, but structural basis remains unknown. Here we present crystal structures destabilase, including a 1.1 Å-resolution structure in complex with ion. Our...
The histone-like (HU) protein is one of the major nucleoid-associated proteins involved in DNA supercoiling and compaction into bacterial nucleoid as well all DNA-dependent transactions. This small positively charged dimeric binds a non-sequence specific manner promoting super-structures. majority HU are highly conserved among bacteria; however, from Mycoplasma gallisepticum (HUMgal) has multiple amino acid substitutions most regions, which believed to contribute its specificity targets...
The histone-like (HU) protein is one of the major nucleoid-associated proteins bacterial nucleoid, which shares high sequence and structural similarity with IHF but differs from latter in DNA-specificity. Here, we perform an analysis structural-dynamic properties HU Spiroplasma melliferum compare its behavior solution to that another mycoplasmal Mycoplasma gallisepticum. high-resolution heteronuclear NMR spectroscopy was coupled molecular-dynamics study comparative thermal denaturation both...
Abstract Human cancers frequently display defects in Ag processing and presentation allowing for immune evasion, they therefore constitute a significant challenge T cell–based immunotherapy. We have previously demonstrated that the antigenicity of tumor-associated Ags can be significantly enhanced through unconventional residue modifications as novel tool MHC class I (MHC-I)–based immunotherapy approaches. also identified category cancer neo-epitopes, is, cell epitopes associated with...
RNA Polymerase III (Pol III) is responsible for transcribing 5S ribosomal (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the rRNA promoter requires transcription factors TFIIIA, TFIIIC, TFIIIB. Here we use cryo-electron microscopy visualize