The micelle-vesicle-micelle transition in aqueous mixtures of the cationic surfactant cetyl trimethyl ammonium bromide (CTAB) and anionic surfactant-like ionic liquid 1-butyl-3-methylimidazolium octyl sulfate, [C4mim][C8SO4] has been investigated by using dynamic light scattering (DLS), transmission electron microscopy (TEM), surface tension, conductivity, fluorescence anisotropy at different volume fractions surfactant. tension value decreases sharply with increasing CTAB concentration up...

Abstract Single-molecule localization microscopy (SMLM) is a rapidly evolving technique to resolve subcellular structures and single-molecule dynamics at the nanoscale. Here, we employ conventional BODIPY conjugates for live-cell SMLM via their previously reported red-shifted ground-state dimers (D II ), which transiently form through bi-molecular encounters emit bright fluorescence. We versatility of D -state nanoscopic spatial regulation single fatty acid analogs (FAas) lipid droplets...

This work demonstrates the utilization of bile salt aggregates as a potential biological host system for studying binding interactions and dynamics poorly-water-soluble drug curcumin by means photophysical techniques. We found that level degradation is greatly suppressed upon encapsulation into nanocavities three different aggregates. However, NaTC are more effective to suppress than NaCh NaDC also report modulation dynamical properties using steady-state time-resolved fluorescence...

The formation of stable unilamellar vesicles which hold great potential for biological as well biomedical applications has been reported in the aqueous mixed solution a surface active ionic liquid (SAIL), 1-hexadecyl-3-methylimidazolium chloride ([C16mim]Cl) and cholesterol. To make comparison we have also shown such using common cationic surfactant, benzyldimethylhexadecylammonium (BHDC) similar alkyl chain length but different headgroup region to that [C16mim]Cl. It revealed from dynamic...

In the recent past, nonaqueous microemulsions containing ionic liquids (ILs) have been utilized for performing chemical reactions, preparation of nanomaterials, and synthesis nanostructured polymers in drug delivery systems. The most promising fact about IL-in-oil is their high thermal stability compared to that aqueous microemulsions. our earlier publication (Rao, V. G.; Ghosh, S.; Ghatak, C.; Mandal, Brahmachari, U.; Sarkar, N. J. Phys. Chem. B 2012, 116, 2850-2855), we presented first...

This article presents the interaction of curcumin in microenvironments provided by aggregation pluronic triblock copolymer P123 into micellar and vesicular assemblies. The formation vesicles using 5-methylsalicylic acid (5 mS) has been successfully characterized optical spectroscopy, light scattering measurement, eventually microscopic techniques. Besides, to make a comparative study between polymeric micelles, we have also investigated photophysical changes F127 micelles having variation...

In this work we have reported the formulation of a novel ionic liquid-in-oil (IL/O) microemulsion where polar core liquid, 1-ethyl-3-methylimidazolium n-butylsulfate ([C2mim][C4SO4]), is stabilized by mixture two nontoxic nonionic surfactants, polyoxyethylene sorbitan monooleate (Tween-80) and laurate (Span-20), in biological oil phase isopropyl myristate (IPM). The formation droplets has been confirmed from dynamic light scattering (DLS) behavior study. To assess heterogeneity tween-based...

A simple procedure for the preparation of giant vesicles using surface active ionic liquids (SAILs) has been provided in this paper.

RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the rRNA promoter requires transcription factors TFIIIA, TFIIIC, TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) visualize S. cerevisiae complex of TFIIIA TFIIIC bound promoter. Gene-specific factor interacts with DNA acts as an adaptor TFIIIC-promoter interactions. We also binding TFIIIB subunits, Brf1 TBP (TATA-box protein), which results in...

The spontaneous micelle-to-vesicle transition in an aqueous mixture of two surface-active ionic liquids (SAILs), namely, 1-butyl-3-methylimidazolium n-octylsulfate ([C4mim][C8SO4]) and 1-dodecyl-3-methylimidazoium chloride ([C12mim]Cl) is described. In addition to detailed structural characterization obtained by using dynamic light scattering, transmission electron microscopy (TEM), cryogenic TEM techniques, ultrafast fluorescence resonance energy transfer (FRET) from coumarin 153 (C153) as...

Autophagy induction involves extensive molecular and membrane reorganization. Despite substantial progress, the mechanism underlying autophagy initiation remains poorly understood. Here, we used quantitative photoactivated localization microscopy with single-molecule sensitivity to analyze nanoscopic distribution of endogenous ULK1, kinase that triggers autophagy. Under amino acid starvation, ULK1 formed large clusters containing up 161 molecules at endoplasmic reticulum. Cross-correlation...

The oxidation of lipids is an important phenomenon with ramifications for disciplines that range from food science to cell biology. development and characterization tools techniques monitor lipid are thus relevant. Of particular significance in this regard facilitate the study oxidations at interfaces heterogeneous samples (e.g., oil-in-water emulsions, membranes). In article, we establish a proof-of-principle methods initiate then such high spatial resolution. experiments were performed...

The modulation of the photophysical properties 1'-hydroxy-2'-acetonaphthone (HAN) upon encapsulation into hydrophobic nanocavities different bile salt aggregates has been investigated for first time using steady-state and time-resolved fluorescence spectroscopy. Because HAN is very sensitive to polarity microenvironment in which it confined, we performed a comparative study on excited-state binding dynamics three salts varying hydrophobicity. led an enhanced intensity along with significant...

Abstract Polyunsaturated fatty acids (PUFAs) are present in lipid systems ranging from cell membranes to food products. Reactive oxygen species (ROS) degrade PUFA‐based lipids, often resulting undesired consequences. Thus, understanding mechanisms of ROS‐mediated oxidation, including means inhibit such important. Of significance oxidation 1) at interfaces heterogeneous samples, and 2) that may propagate one droplet another an emulsion. We used optical technique study spatially resolved...

In this manuscript, we have modulated the location of an anionic probe, Coumarin-343 (C-343) in a zwitterionic (N-hexadecyl-N,N-dimethylammonio-1-propanesulfonate (SB-16)) micellar system by three different approaches. The effect addition surfactant sodium dodecyl sulfate (SDS) and room temperature ionic liquid (RTIL), 1-ethyl-3-methylimidazolium octylsulfate (EmimOs) N,N-dimethylethanol hexanoate (DAH), to solution has been studied. pH variation studied as well using solvent rotational...

The dynamic rearrangement of chromatin is critical for gene regulation, but mapping both the spatial organization and its dynamics remains a challenge. Many structural conformations are too small to be resolved via conventional fluorescence microscopy long acquisition time super-resolution photoactivated localization (PALM) precludes characterization below optical diffraction limit in living cells due motion. Here we develop correlative PALM imaging approach quantitatively map time-averaged...

The ability of cells to respond external signals is essential for cellular development, growth, and survival. To a signal from the environment, cell must be able recognize process it. This task mainly relies on function membrane receptors, whose role convert into biochemical language cell. G protein-coupled receptors (GPCRs) constitute largest family receptor proteins in humans. Among GPCRs, metabotropic glutamate (mGluRs) are unique subclass that as obligate dimers possess large...