Moringa oleifera is a tree distributed in Mexican semiarid and coastal regions. M. used practice the treatment of various diseases available without medical prescription, often form an herbal infusion for everyday use. The aim present study was to evaluate chemical composition nutritional values dried leaf powder collected from two different regions Mexico. All samples exhibited moisture levels varying 3.06 3.34%, lipids 10.21 10.31%, fiber 7.29 9.46%, ashes 10.71 11.18%, crude protein 10.74...

Leishmanicidal drugs have many side effects, and drug resistance to all of them has been documented. Therefore, the development new identification novel therapeutic targets are urgently needed. Leishmania mexicana trypanothione reductase (LmTR), a NADPH-dependent flavoprotein oxidoreductase important thiol metabolism, is essential for parasite viability. Its absence in mammalian host makes this enzyme an attractive target anti-Leishmania drugs. Herein, tridimensional model LmTR was...

In this study, the potential use of Moringa oleifera as a clotting agent different types milk (whole, skim, and soy milk) was investigated. M. seed extract showed high milk-clotting activity followed by flower extract. Specific 200 times higher than that Seed is composed four main protein bands (43.6, 32.2, 19.4, 16.3 kDa). Caseinolytic assessed sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) tyrosine quantification, extent casein degradation using Milk cheese soft...

Mitochondrial dysfunction is a hallmark of diabetes, but the metabolic alterations during early stages disease remain unknown. The ability liver cells to rearrange their metabolism plays an important role in compensating energy shortage and may provide cell survival. Moringa oleifera leaves have been studied for its health properties against insulin resistance, non-alcoholic disease. We postulated that M. executes protective function on mitochondrial functionality HepG2 treated with high...

The increasing prevalence of diabetes continues to be a major health issue worldwide. Alteration mitochondrial electron transport chain is recognized hallmark the diabetic-associated decline in liver bioenergetics; however, molecular events involved are only poorly understood. Moringa oleifera used for treatment diabetes. However, its role on functionality not yet established. This study was aimed evaluate effect M. extract supercomplex formation, ATPase activity, ROS production, GSH levels,...

In our study, we aimed to evaluate the effects of Moringa oleifera leaves extract on rat paraoxonase 1 (rPON1) and catalase (rCAT) activities in alloxan-induced diabetic rats. Our study included three groups; group C (control, n = 5); D (diabetic, DM (M. extract-supplemented rats, 5). Daily oral administration M. at 200 mg/kg doses produced an increase endogenous antioxidants. Serum rPON1 (lactonase) liver cytosol were determined by a spectrophotometric assay using progress curve analysis....

Preeclampsia is a pregnancy-specific disorder in humans and major cause of maternal neonatal morbidity mortality.Increasing evidence suggests that oxidative stress plays an important role the pathogenesis preeclampsia.The aim this study was to investigate relationship between null alleles glutathione S-transferases (GST) M1 T1 genes risk preeclampsia.This case-control involved 112 preeclamptic 233 normoevolutive pregnant women.The polymorphisms were 2161 GSTT1/GSTM1 preeclampsia ©FUNPEC-RP...

Uric acid has been associated as a risk factor for cardiovascular disease. Recently, however, there is growing evidence that uric plays role antioxidant in the brain. In cognitive dysfunction, vascular and oxidative stress mechanisms play role, but link remains unknown. Therefore, we investigated between serum acid-levels function 62 elderly subjects. The statistical analysis was adjusted to age, sex factors. Here, found lower levels are linked dysfunction. Mexican population, higher of with...

Exposure to arsenic in drinking water has been associated with various complications of pregnancy including fetal loss, low birth weight, anemia, gestational diabetes and spontaneous abortion. However, date, there are no studies evaluating its possible association preeclampsia. This case–control study involved 104 preeclamptic 202 healthy pregnant women. The concentrations urine were measured using a Microwave Plasma-Atomic Emission Spectrometer. We found relatively levels household tap...

Methicillin-resistant Staphylococcus auerus (MRSA) strains are having a major impact worldwide, and due to their resistance all β-lactams, an urgent need for new drugs is emerging. In this regard, the shikimate pathway considered be one of metabolic features bacteria absent in humans. Therefore enzymes involved route, such as dehydrogenase (SDH), excellent targets discovery novel antibacterial drugs. study, SDH from MRSA (SaSDH) was characterized. The results showed that enzyme monomer with...

Human African Trypanosomiasis (HAT), a disease that provokes 2184 new cases year in Sub-Saharan Africa, is caused by Trypanosoma brucei. Current treatments are limited, highly toxic, and parasite strains resistant to them emerging. Therefore, there an urgency find drugs against HAT. In this context, T. brucei depends on glycolysis as the unique source for ATP supply; therefore, enzyme triosephosphate isomerase (TIM) attractive target drug design. present work, three benzimidazole derivatives...

Protein kinase B also known as AKT is a cardinal node in different signaling pathways that regulates diverse cell processes. has three isoforms share high homology. Hyperactivation of each isoform related with types cancer. This work describes the computational search for new inhibitors using hit optimization process previously reported pan inhibitor, 2,4,6-trisubstituted pyridine. A database molecules was proposed variant fragment-based docking methodology and previous considerations....

Cap color is an important commercial trait for oyster mushrooms. Various pigment constituents determine a diverse color. However, the pigments of mushrooms are still ambiguous. The pink mushroom ( Pleurotus salmoneostramineus or djamor) chromoprotein one few proteins belonging to this fungus that has record its sequence amino acid residues. even though there studies about isolation, purification, and crystallization, current information focused on 3-dimensional model cofactor prosthetic...

The soil microorganism Myxobacter Sp. AL-1 regulates in a differential manner the production of five extracellular cellulases during its life cycle. nucleotide sequence cel9-cel48 cluster from genome this was recently obtained. Cel48 expressed Escherichia coli to generate His(6)-Cel48 protein and biochemical properties pure were determined. more efficient degrading acid-swollen avicel (ASC) than carboxymethylcellulose (CMC). On other hand, cel9 Bacillus subtilis an IPTG-inducible promoter....

Variations in genes involved DNA repair systems have been proposed as risk factors for the development of preeclampsia (PE). We conducted a case-control study to investigate association Human apurinic/apyrimidinic (AP) endonuclease (APEX1) Asp148Glu (rs1130409), Xeroderma Pigmentosum group D (XPD) Lys751Gln (rs13181), X-ray cross-complementing 1 (XRCC) Arg399Gln (rs25487) and 3 (XRCC3) Thr241Met (rs861539) polymorphisms with PE Mexican population. Samples 202 cases 350 controls were...

Entamoeba histolytica, the parasite which causes amebiasis is responsible for 110 000 deaths a year. histolytica depends on glycolysis to obtain ATP cellular work. According metabolic flux studies, hexokinase exerts highest control of this pathway; therefore, it an excellent target in search new antiamebic drugs. To end, tridimensional model E. 1 (EhHK1) was constructed and validated by homology modeling. After virtual screening 14 400 small molecules, 100 with best docking scores were...

The yeast plasma membrane H+-ATPase isolation procedure was improved; a highly pure enzyme (90−95%) obtained after centrifugation on trehalose concentration gradient. kinetics slightly cooperative: Hill number = 1.5, S0.5 800 μM ATP, and turnover 36 s-1. In contrast to those of other P-type ATPases, fluorescence sensitive nucleotide binding; the decreased 60% in presence both 5 mM ADP AMP-PNP. Fluorescence titration with nucleotides allowed calculation dissociation constants (Kd) from...

Abstract Due to its resistance many antibiotics, methicillin‐resistant Staphylococcus aureus ( MRSA ) have become a worldwide health problem creating the urgent necessity of developing new drugs against this pathogen. In sense, one approach is search for inhibitors important enzymes in metabolism. According this, shikimate pathway an metabolic route bacteria and are considered as great targets development antibiotic drugs. One these dehydrogenase that catalyzes reversal reduction from...