A5053876476- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Protein purification and stability
- Microbial Metabolic Engineering and Bioproduction
- Microfluidic and Bio-sensing Technologies
- thermodynamics and calorimetric analyses
- Animal Disease Management and Epidemiology
- Advanced Control Systems Optimization
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Osteoarthritis Treatment and Mechanisms
- Cell death mechanisms and regulation
- Trypanosoma species research and implications
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Electrical and Bioimpedance Tomography
- Periodontal Regeneration and Treatments
- CRISPR and Genetic Engineering
- Anaerobic Digestion and Biogas Production
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Click Chemistry and Applications
- Viral gastroenteritis research and epidemiology
Universitat Autònoma de Barcelona
2012-2021
Clínica Girona
2012-2020
Centre de Recerca Matemàtica
2001
Universitat de Barcelona
1992-1995
Consejo Superior de Investigaciones Científicas
1994
Centro de Biología Molecular Severo Ochoa
1994
Universidad Autónoma de Madrid
1994
Abstract The formulation of the culture medium for a Chinese hamster ovary (CHO) cell line has been investigated in terms simultaneous replacement glucose and glutamine, most commonly employed carbon nitrogen sources, pursuing objective achieving more efficient use these compounds, simultaneously avoiding accumulation lactate ammonium medium. key factor this process is selection compounds that are slowly metabolized. Among different studied, galactose glutamate provide best results, allowing...
The effect of glutamine replacement by glutamate and the balance between glucose metabolism on redistribution t-PA-producing recombinant CHO cells is studied in a series shift down up experiments continuous culture. These reveal existence multiple steady states, experimental data are used to perform metabolic flux analysis gain better insight into cellular its redistribution. Regulation feed rate promotes higher efficiency nitrogen source utilization, with lower production byproducts, but...
The Mediterranean basin is an endemic region for canine leishmaniosis (CanL), where it represents a major veterinary problem and raises human health concerns. However, the distribution of disease heterogeneous not all countries locations have been equally studied characterized. This work describes situation CanL in Girona province (Catalonia, Spain), which no data has previously reported, presents relevant study to exemplify other areas with similar characteristics across region. Four...
At early stages of the exponential growth phase in HEK293 cell cultures, tricarboxylic acid cycle is unable to process all amount NADH generated glycolysis pathway, being lactate main by-product. However, cells are also able metabolize depending on environmental conditions. It has been recently observed that one most important modes metabolization cometabolism and glucose, even during phase. Extracellular concentration pH appear be key factors triggering metabolic shift from glucose...
Abstract The development of a strategy for the culture Chinese hamster ovary (CHO) cells producing tissue plasminogen activator (t‐PA) is investigated. This based on replacement main carbon source, glucose, by another compound that slowly metabolizable, particularly galactose. introduction this change allows acute in cell behavior at various levels. Cell growth stopped after nutrient shift, and can be kept long‐duration low rate high viability as compared with solely glucose utilization....
Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one the FGFs with highest mitogenic activity.We studied effect on MSCs pluripotency. duplication time (Td) was significantly reduced compared to controls (2.2 ± 0.2 vs. 4.1 days, respectively; p = 0.03) while BMP-2 SCF-1 did not exert significant effect....
Abstract BACKGROUND Improvement of the mammalian cell culture process is based on implementation strategies with higher productivities, such as fed‐batch or perfusion processes. The efficient development these depends strongly availability suitable monitoring tools to determine activity and adjust nutrient feeding correspondingly . RESULTS In this study oxygen uptake rate ( OUR ), density glucose concentration on‐line systems are applied control Different combinations were used growth...
Peptides representing the sequence of immunodominant loop foot‐and‐mouth disease virus strain C‐S8 (YTASARGDLAHLTTTHARHLP, residues 136–156 VP1) and several variant viruses have been prepared by solid phase methods. In addition, five peptides with single‐residue replacements at Leu 147 (Ile, Nle, Val, Ala, Gly) synthesized. Tosyl dinitrophenyl protections for histidine compared, latter being found to give better synthetic products. The tested in an immunodot assay against a panel monoclonal...
The aim of this work is to provide optimization tools for cell and tissue engineering processes through continuous monitoring cultures. Structural properties can be obtained from non-destructive electrical measurements by using impedance spectroscopy (EIS). EIS on monolayer animal cultures are usually performed a two-electrode strategy. Because this, the measurement very sensitive electrode covering ratio degree adherence cells. Of course, these parameters give useful information but mask...