Cerebrospinal fluid conveys many physiologically important signaling factors through the ventricular cavities of brain. We investigated transport cerebrospinal in third ventricle mouse brain and discovered a highly organized pattern cilia modules, which collectively give rise to network flows that allows for precise within this ventricle. also cilia-based switch reliably periodically alters flow so as create dynamic subdivision may control substance distribution Complex patterns were present...

The rhizosphere-associated bacterium Serratia plymuthica HRO-C48 is not only able to suppress symptoms caused by soil-borne pathogens but also stimulate growth of plants. Detailed knowledge about the underlying mechanisms and regulation are crucial for application in biocontrol strategies. To analyse influence N-acyl homoserine lactone (AHL)-mediated communication on activity, AHL-degrading lactonase AiiA was heterologously expressed strain, resulting abolished AHL production. comparative...

The rapid reorganization of the actin cytoskeleton in response to external stimuli is an essential property many motile eukaryotic cells. Here, we report evidence that machinery chemotactic Dictyostelium cells operates close oscillatory instability. When averaging a short pulse chemoattractant cAMP, observed transient accumulation cortical reminiscent damped oscillation. At single-cell level, however, dynamics ranged from short, strongly responses slowly decaying, weakly oscillations....

Bacteria frequently colonize natural microcavities such as gut crypts, plant apoplasts, and soil pores. Recent studies have shown that the physical structure of these spaces plays a crucial role in shaping stability resilience microbial populations (Karita et al., PNAS 2022, Postek 2024). Here, we demonstrate protected microhabitats can emerge dynamically, even absence barriers. Our numerical analytical models reveal this self-organization persists when strains different growth rates,...

Background: Oral biofilm studies based on simplified experimental setups are difficult to interpret. Models limited mostly by the number of bacterial species observed and insufficiency artificial media. Few have attempted overcome these limitations cultivate native oral biofilm. Aims: This study aimed grow in vivo before transfer a reactor for ex situ incubation. The vitro survival this changes composition over time were observed. Methods: Six human enamel-dentin slabs embedded buccally...

Chemotactic responses of Dictyostelium discoideum cells to periodic self-generated signals extracellular cAMP comprise a large number intricate morphological changes on different length scales. Here, we scrutinized chemotaxis single under conditions starvation using variety optical, electrical and acoustic methods. Amebas were seeded gold electrodes displaying impedance oscillations that simultaneously analyzed by optical video microscopy relate synchronous in cell density, morphology,...

Biological systems with their complex biochemical networks are known to be intrinsically noisy. Here we investigate the dynamics of actin polymerization amoeboid cells, which close onset oscillations. We show that large phenotypic variability in can accurately captured by a generic nonlinear oscillator model presence noise. determine relative role noise single dimensionless, experimentally accessible parameter, thus providing quantitative description population cells. Our approach, rests on...

Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol oil overlay method. In addition, present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate...

Time-resolved analysis employing electric cell–substrate impedance sensing reveals periodic changes of small ensembles Dictyostelium discoideum amoebae oscillating synchronously upon deprivation food. Impedance are attributed to alter­ations cell shape and distance. Ultrasmall electrodes allow the detection single traces. Detailed facts importance specialist readers published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available...

The chemotactic motion of eukaryotic cells such as leukocytes or metastatic cancer relies on membrane protrusions driven by the polymerization and depolymerization actin. Here we show that response actin system to a receptor stimulus is subject threshold value varies strongly from cell cell. Above threshold, observe pronounced cell-to-cell variability in amplitude. time, however, almost constant over entire range amplitudes, while time increases with increasing We amplitude correlates amount...

The clade of Myxogastria, commonly described as true or plasmodial slime molds, contains more than 1000 species. During their life cycle many these molds develop extended networks connected veins, known unicellular (phanero)plasmodia. Among those, Physarum polycephalum gathered by far the most attention biologists and physicists. Via oscillating cytoplasmic streams nutrients well signaling factors spread through adaptive network. These properties have rendered it not only a model organism...

We have compared Physarum polycephalum to other plasmodial slime molds, based on several properties of their respective phaneroplasmodia, as well chemotactic responses. This work was presented at PhysNet 2015.

This work studies the response of migratory machinery in chemotactic cells to a short stimulus with chemo-attractant. In exhibiting noisy oscillations before stimulation, authors observe long transient response, cell returns back its initial oscillatory state. The properties system measured experimentally are well reproduced help model based on generic nonlinear oscillator

Dictyostelium discoideum cells respond to periodic signals of extracellular cAMP by collective changes cell-cell and cell-substrate contacts. This was confirmed dielectric analysis employing electric impedance sensing (ECIS) measurements involving cell-filled micro channels in conjunction with optical microscopy providing a comprehensive picture chemotaxis under conditions starvation.