A5063972135- Cardiac electrophysiology and arrhythmias
- Fatty Acid Research and Health
- Ion channel regulation and function
- Cardiovascular Function and Risk Factors
- Phytoestrogen effects and research
- Cardiac Ischemia and Reperfusion
- Diet, Metabolism, and Disease
- Antioxidant Activity and Oxidative Stress
- Nuclear Structure and Function
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Nutritional Studies and Diet
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Cardiomyopathy and Myosin Studies
- Reproductive tract infections research
- Eicosanoids and Hypertension Pharmacology
- ATP Synthase and ATPases Research
- Trace Elements in Health
- Vitamin C and Antioxidants Research
- Ion Transport and Channel Regulation
- Biochemical effects in animals
- Protease and Inhibitor Mechanisms
University of Manitoba
2016-2025
St. Boniface Hospital
2016-2025
Cardiovascular Institute Hospital
2022
Agriculture and Agri-Food Canada
2006-2021
Research Manitoba
2015-2020
Canadian Science Centre for Human and Animal Health
2014-2018
Clinical Research Institute
2014-2015
University of Winnipeg
2014
University of Holguín
2009-2013
University of Colorado Boulder
2007
Epidemiological investigations have linked Chlamydia pneumoniae infection to atherosclerosis. It is not clear, however, whether C. plays a causal role in the development of Mice with low-density lipoprotein receptor deficiency were induced develop atherosclerotic lesions aorta cholesterol-enriched diet that increased serum cholesterol by two- threefold. Using this mouse model, we found chlamydial alone either AR39 or trachomatis MoPn strain failed induce any significant over period nine...
The effects of insulin and thyroid hormone treatments on cardiac sarcoplasmic reticular function were investigated in chronic streptozotocin-induced diabetes rats. ATP-dependent Ca2+ transport Ca2+-stimulated ATPase activities depressed significantly microsomal samples from diabetic rats comparison with control (P less than 0.05). This defect was seen at various times incubation (1-20 min) different concentrations free (10(-7) to 10(-5) M Ca2+) accompanied by changes the protein composition...
Flaxseed contains ω-3 fatty acids, lignans, and fiber that together may provide benefits to patients with cardiovascular disease. Animal work identified peripheral artery disease particularly benefit from dietary supplementation flaxseed. Hypertension is commonly associated The purpose of the study was examine effects daily ingestion flaxseed on systolic (SBP) diastolic blood pressure (DBP) in patients. In this prospective, double-blinded, placebo-controlled, randomized trial, (110 total)...
Dietary flaxseed has been shown to have potent antiatherogenic effects in rabbits. The purpose of the present study was investigate capacity an animal model that more closely represents human atherosclerotic condition, LDL receptor-deficient mouse (LDLrKO), and identify cellular mechanisms for these effects. LDLrKO mice were administered a regular diet (RG), 10% flaxseed-supplemented (FX), or atherogenic containing 2% cholesterol alone (CH) supplemented with (CF), 5% (CF5), 1% (CF1), coconut...
The impact of dietary polyunsaturated fatty acids (PUFAs) the n-6 and n-3 series on cardiovascular system is well documented. To directly compare effects three oils (fish, flaxseed hempseed) given in concentrations expected to be self-administered general population specific parameters healthy volunteers.86 male female volunteers completed a 12 week double blinded, placebo controlled, clinical trial. They were randomly assigned one four groups. Subjects orally supplemented with two 1 gm...
Heart sarcolemmal membranes were isolated by the hypotonic shock-LiBr treatment from rats with chronic diabetes induced a streptozotocin (65 mg/kg, iv) injection. Sarcolemmal Mg2+-dependent ATPase activity was elevated, whereas 5'-nucleotidase and K+-p-nitrophenylphosphatase activities in diabetic heart depressed comparison to control preparations. Although patent Na+-K+-ATPase ouabain-sensitive unaltered, latent activities, as determined after alamethicin or deoxycholate treatments, found...
In a randomized, double-blinded, controlled clinical trial, participants with peripheral arterial disease (75% hypertensive) consumed 30 g of milled flaxseed/d for 6 months. The flaxseed group exhibited significant reductions in systolic (−10 mm Hg) and diastolic (−7 blood pressure. Flaxseed contains the n3 fatty acid α-linolenic acid. Plasma increased ingestion was inversely associated However, antihypertensive mechanism unclear. Oxylipins derived from polyunsaturated acids regulate...
Myocardial ischemia-reperfusion (I/R) injury increases the generation of oxidized phosphatidylcholines (OxPCs), which results in cell death. However, mechanism by OxPCs mediate death and cardiac dysfunction is largely unknown. The aim this study was to determine mechanisms OxPC triggers cardiomyocyte during reperfusion injury. Adult rat ventricular cardiomyocytes were treated with increasing concentrations various purified fragmented OxPCs. Cardiomyocyte viability, bioenergetic response,...
Background: Dietary flaxseed may have significant health-related benefits due to its high content of the omega-3 fatty acid, alpha-linolenic acid (ALA). However, before extensive work can be undertaken in clinical populations determine efficacy, basic information on ALA bioavailability from and physiological effects ingestion need examined.Objective: The purpose this study, therefore, was when ingested form whole seed, milled seed or as oil.Design: components (30 g 6 oil) were baked into...
Chronic streptozotocin-induced diabetes in rats was associated with a significant loss the ability of isolated cardiac sarcolemmal membranes to bind Ca2+. Administration insulin diabetic normalized Ca2+ binding capacity. The content sialic acid residues, which are considered represent superficial pool sarcolemma, decreased preparations from rats, and this change also reversible upon treatment rats. Treatment sarcolemma neuraminidase by 37% control but had no effect on preparations....
Selected substrate and hormonal responses to exercise were compared in two phases of the menstrual cycle. Exercise-induced changes [glucose, lactate, free fatty acids (FFA), glycerol] patterns [luteinizing hormone (LH), follicle-stimulating (FSH), insulin, progesterone (P), growth (GH), cortisol] follicular luteal cycle 24-h-fasted (n = 5), glucose-loaded 6; 1.50 g/kg, 20% solution), control subjects 8). A treadmill walk was maintained for 60 min (30 min, 40% VO2 max; 30 80% max). Blood...