A5023626117- DNA Repair Mechanisms
- BRCA gene mutations in cancer
- CRISPR and Genetic Engineering
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Genomics and Chromatin Dynamics
- Autophagy in Disease and Therapy
- Colorectal Cancer Screening and Detection
- Epigenetics and DNA Methylation
- PARP inhibition in cancer therapy
- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Colorectal Cancer Treatments and Studies
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
Centro di Riferimento Oncologico
2009-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2002-2021
PCR Oncology
2012
National Cancer Institute
2009-2011
Breast Cancer Now
2005
Institute of Cancer Research
2005
Breast Cancer Research Foundation
2004
Inactivation of p53 contributes significantly to the dismal prognosis breast tumors, most notably triple-negative cancers (TNBCs). How relief from tumor suppressive functions results in cell aggressive behavior is only partially elucidated. In an attempt shed light on implication microRNAs this context, we discovered a new signaling axis involving p53, miR-30a and ZEB2. By silico approach identified as putative target observed that tumors reduced expression correlated with inactivation,...
BACKGROUND Active specific immunotherapy (ASI) is a strategy that attempts to boost the host's immune response specifically against its own tumor. The purpose of this study was investigate effect adjuvant ASI in patients with renal carcinoma. METHODS Of 120 consecutive patients, 60 were randomized control group and receive comprised 3 intradermal injections 107 autologous irradiated tumor cells mixed Bacillus Calmette-Guèrin (in first 2 vaccinations) or alone. At randomization 1, 6, 12...
Loss of expression miR‐200 family members has been implicated in cellular plasticity, a phenomenon that accounts for epithelial‐to‐mesenchymal transition (EMT) and stem‐like features many carcinomas is considered major cause tumor aggressiveness drug resistance. Nevertheless, the mechanisms downregulation breast cancer are still largely unknown. Here we show miR‐200c inversely correlates with miR‐200c/miR‐141 locus methylation triple‐negative tumors (TNBC). Importantly, low levels high...
Increasing evidence indicates that invasive properties of breast cancers rely on gain mesenchymal and stem features, which has suggested the dual targeting these phenotypes may represent an appealing therapeutic strategy. It is known fraction cells can be enriched by culturing cancer as mammospheres (MS), but whether pro-stem conditions favor also expansion provided features still undefined. In attempt to shed light this issue, we compared a panel 10 cell lines representative distinct...
Autophagy is a conserved catabolic process that controls organelle quality, removes misfolded or abnormally aggregated proteins and part of the defense mechanisms against intracellular pathogens. contributes to suppression tumor initiation by promoting genome stability, cellular integrity, redox balance proteostasis. On other hand, once established, autophagy can support cancer cell survival promote epithelial‑to‑mesenchymal transition. A growing number molecules involved in have been...
The heritable defects of BRCA1 and BRCA2 genes have been shown to predispose breast ovarian cancers. In a previous report, we analyzed 46 Italian families with and/or cancer for mutations. the present study, those 11 others were screened mutations; newly enrolled also gene. coding region splice boundaries assessed by protein-truncation test single-strand conformational polymorphism. A total 20 different mutations found in 21 (37%). 9 (16%) showed gene, including one new mutation identified...
Inactivation of the breast cancer susceptibility gene 1 (BRCA1) plays a significant role in development subset familial and ovarian cancers, but increasing evidence points to also sporadic tumors. BRCA1 is multifunctional nuclear protein involved regulation many cellular processes, including DNA repair, cell cycle, transcription chromatin remodeling. To identify novel proteins participating network, two-dimensional gel electrophoresis MALDI-TOF mass spectrometry were used compare...
BRCA1-associated tumors are characterized by an elevated genomic instability and peculiar expression profiles. Nevertheless, tailored treatments for BRCA1 mutation carriers have only been partially investigated up to now. The implementation of therapeutic strategies specific these patients has in part hindered the paucity proper preneoplastic neoplastic BRCA1-deficient tumor cell models. In this study, we took advantage RNA interference technology generate a series transformed (HBL100)...
Autophagy is an intracellular catabolic process that increasingly being recognized as a crucial factor in several human diseases including cancers. Mounting evidence suggests autophagy allows tumor cells to overcome otherwise fatal stresses and increase dissemination. Nevertheless, how controls these processes particular it impinges on cell-cell adhesion still poorly understood. Here, we investigate the role of turnover epithelial molecule E-cadherin context breast cancer. We demonstrated...
Abstract We examined ICAM‐1 expression in 37 freshly dissociated renal‐cancer cell populations. Immunoperoxidase analysis revealed that 31 of the renal tumors expressed ICAM‐I to various degrees; was significantly lower tumor cells obtained from patients remaining tumor‐free after a median follow‐up 60 months (mean value 24.4% ± 21) than relapsed 40.8% 22), and low this molecule on surface seemed correlate with favorable clinical behavior. In 41 patients, mean level sICAM‐1 551 260 ng/ml,...
Hereditary non-polyposis colorectal cancer (HNPCC) is a genetically heterogeneous disease for which PMS2 gene, member of the human PMS gene family, believed to have marginal role. To better define contribution hereditary cancer, we investigated this in 22 unrelated Italian patients that, despite positive family history and/or early onset and development tumors with microsatellite instability (MSI), did not carry constitutional mutations MLH1 MSH2 genes. No clear-cut pathogenetic significance...
Most familial breast or ovarian cancers are thought to be due highly penetrant mutations in the predisposing genes BRCA1 and BRCA2. The cloning of these has opened a new era for genetic counseling women with family history cancer. To estimate incidence detectable define eligibility criteria testing Italian population, total 53 patients belonging 46 families clustering multiple cases and/or cancer were investigated. Seven presented only, 16 had both cancers, 23 characterized by only. Using...
Abstract Background Breast cancer in men is an infrequent occurrence, accounting for ~1% of all breast tumors with incidence about 1:100,000. The relative rarity male (MBC) limits our understanding the epidemiologic, genetic and clinical features this tumor. Methods From 1997 to 2003, 10 MBC patients were referred Institute counselling BRCA1/2 testing. Here we report on phenotypic characterization families from North East Italy. In particular, wished assess occurrence specific types...
Aims and Background Colorectal carcinoma patients from hereditary non-polyposis colorectal cancer families are suggested to have a better prognosis than sporadic cases. Since the majority of cancer-related carcinomas characterized by microsatellite instability due germline mutations in DNA mismatch repair genes, this is consistent with prolonged survival observed instability-positive compared stable However, fraction cases belongs that, despite fulfilling clinical criteria for cancer, do not...
DNA methylation is an important epigenetic mechanism of transcriptional control that plays essential role in several cellular functions. Aberrant cancer has been frequently associated with downregulation microRNAs and protein coding genes, such as miR-200c/miR-141 cluster E-cadherin. Current strategies to assess methylation, including bisulfite treatment-based assays, tend be time-consuming may quite expensive when a precise appraisal required. The Sanger-sequencing the amplified...