Colored wheat has been recognized broadly for its nutritional value because of natural content the colorant anthocyanin. To investigate reasons formation grain color at maturity, metabolomic and transcriptomic analyses were performed on three different colors wheat. Through metabolome analysis, 628 metabolites identified. Of 102 flavonoids, there are 9 kinds anthocyanins related to formation, mainly cyanidin peonidin, their metabolite was lowest in white-grain Among genes associated with...

The detection of oil in water is great importance for maintaining subsurface infrastructures such as pipelines. As a potential technology oceanic application, an lidar has proved its advantages remote sensing optical properties and subsea materials. However, current systems are highly power-consuming bulky, making them difficult to deploy underwater monitor water. To address this issue, we have developed compact single-photon Raman by using detector with high quantum efficiency low dark...

Abstract Background Quinoa ( Chenopodium quinoa Willd.) originates in high altitude areas, such as the Andes, and has some inherent characteristics of cold, drought, salinity tolerance, but is sensitive to temperature. Results To gain insight into response mechanism temperature stress, we conducted an extensive targeted metabolomic study two cultivars, Dianli-3101 Dianli-3051, along with a combined transcriptome analysis. A total 794 metabolites 54,200 genes were detected, which related...

Abstract Prokaryotic heme biosynthesis in Gram‐positive bacteria follows the coproporphyrin‐dependent pathway. The last step this pathway is catalyzed by enzyme coproheme decarboxylase, which oxidatively transforms two propionate groups into vinyl yielding b . catalytic reaction cycle of decarboxylases exhibits four different states: apo‐form, substrate (coproheme)‐bound form, a transient three‐propionate intermediate form (monovinyl, monopropionate deuteroheme; MMD), and product (heme...

Abstract Polymyxins are last‐resort antimicrobial peptides administered clinically against multi‐drug resistant bacteria, specifically in the case of Gram‐negative species. However, an increasing number these pathogens employ a defense strategy that involves relay enzymes encoded by pmrE ( ugd ) loci and arnBCDTEF operon. The pathway modifies lipid‐A component outer membrane (OM) lipopolysaccharide (LPS) adding 4‐amino‐4‐deoxy‐ l ‐arabinose L ‐Ara4N) headgroup, which renders polymyxins...

Yunnan hulled wheat (YHW) is a wild ancestor of cultivated and possesses rich genetic variation; however, there limited research on teff at the molecular level. Therefore, in this study, two lines with large differences kernel size were used as materials, their kernels sampled 7, 21, 35, 49 days after flowering; surface area thousand weight measured analyzed; transcriptome sequencing analysis was also performed, which showed that total 88,801 genes annotated by Kyoto Encyclopedia Genes...

Gram-negative bacteria harness the proton motive force (PMF) within their inner membrane (IM) to uphold cell envelope integrity, an indispensable aspect for both division and survival. The IM TolQ-TolR complex is essential part of Tol-Pal system, serving as a conduit PMF energy transfer outer membrane. Here we present cryo–electron microscopy reconstructions Acinetobacter baumannii TolQ in apo TolR-bound forms at atomic resolution. configuration manifests symmetric pentameric pore, featuring...

Quinoa (Chenopodium quinoa Willd.) is an herb of the genus Chenopodiaceae that native to Andes Mountains South America. To understand metabolic differences between various strains, we selected strains four colors (black, red, yellow, and white) subjected seeds extensive targeted metabolomics analysis using liquid chromatography-tandem mass spectrometry transcriptomics analysis. In total, 90 flavonoid-related metabolites were detected in colors. We elucida ted regulatory mechanisms flavonoid...

Laser-induced fluorescence (LIF) technology has been widely applied in remote sensing of aquatic phytoplankton. However, due to the weak signal induced by laser excitation and significant attenuation water, profiling detection becomes challenging. Moreover, it remains difficult simultaneously retrieve coefficient ( K l i d a r m f ) volume scattering function at 180° β through single lidar. To address these issues, novel all-fiber oceanic lidar is proposed, characterized by: 1) obtaining...

For several well-documented reasons, it has been challenging to develop artificial small molecule inhibitors of protein/protein complexes. Such reagents are particular interest for transcription factor complexes given links between their misregulation and disease. Here we report parallel approaches identify regulators a hypoxia signaling complex, involving the ARNT subunit HIF (Hypoxia Inducible Factor) activator TACC3 (Transforming Acidic Coiled Coil Containing Protein 3) coactivator. In...

Background Dyslipidemia is an extremely prevalent but preventable risk factor for cardiovascular disease. However, many dyslipidemia patients remain undetected in resource limited settings. The study was performed to develop and evaluate a simple effective prediction approach without biochemical parameters identify those at high of rural adult population. Methods Demographic, dietary lifestyle, anthropometric data were collected by cross-sectional survey from 8,914 participants living areas...

TAp63 is a tumour-suppressor protein that often underexpressed in various types of cancer. It has been shown to activate gene transcription depending on the domain and be closely related with metastasis. In this study, we demonstrate suppresses metastasis colon cancer cells through microRNA-133b. We evaluated correlation miR-133b HT-29 SW-620 investigated roles expression RhoA, E-cadherin vimentin. further TAp63-mediated invasion migration cells. downregulated cancer, microRNA-133b...

The crop production of quinoa (Chenopodium Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand tolerance mechanism quinoa, we screened drought-tolerant genotype "Dianli 129" and studied seedling leaves after rewatering treatments using transcriptomics targeted metabolomics. Drought-treatment, control, rewatering-treated, rewatered control were named as DR, DC, RW, RC, respectively. Among four comparison groups, DC vs. RC...

Quinoa, a cool-weather high-altitude crop, is susceptible to low-temperature stress throughout its reproductive phase. Herein, we performed broadly targeted metabolic profiling of quinoa seedlings explore the metabolites’ dynamics in response and transcriptome analysis determine underlying genetic mechanisms. Two variants, namely, Dian Quinoa 2324 281, were exposed temperatures −2, 5, 22 °C. A total 794 metabolites detected; 52,845 genes, including 6628 novel annotated using UPLC-MS/MS...

A lidar technique has been proposed and demonstrated for remotely sensing particulate beam attenuation coefficient (cp) profiles using the Raman backscattered signal from water. In lidar, backscatter at 180° can be considered constant, allowing determination of (Klidar) signal. This scheme these features. 1) The bandwidth filter that used to extract component was optimized ensure sufficient strength while minimizing influence chlorophyll fluorescence on inversion. 2) receiving telescope with...

Abstract Gram-negative bacteria harness the proton motive force (PMF) within their inner membrane (IM) to uphold integrity of cell envelope, an indispensable aspect for both division and survival. The IM TolQ-TolR complex is essential part Tol-Pal system, serving as a conduit PMF energy transfer outer membrane. Here we present cryo-EM reconstructions Acinetobacter baumannii TolQ in apo TolR- bound forms at atomic resolution. configuration manifests symmetric pentameric pore, featuring...

The hypoxia-inducible factor complex (HIF-α·aryl hydrocarbon receptor nuclear translocator (ARNT)) requires association with several transcription coactivators for a successful cellular response to hypoxic stress. In addition the conventional global coactivator CREB-binding protein/p300 (CBP/p300) that binds HIF-α transactivation domain, new group of called coiled-coil (CCCs) interact directly second PER-ARNT-SIM (PAS) domain ARNT (ARNT PAS-B). These less studied play essential roles in...

A novel oceanic fluorescence lidar technique has been proposed and demonstrated for remotely sensing the volume scattering function at 180° ( β f ), which can be used to further retrieve profiles of absorption coefficient phytoplankton a ph ) 532 nm chlorophyll concentration (Chl). This scheme these features. 1) The single-photon detection technology is employed enhance sensitivity level, enabling obtain backscatter profiles. 2) In terms algorithms, Raman backscattered signals water are...

Lidar has emerged as a promising technique for vertically profiling optical parameters in water. The application of single-photon technology enabled the development compact oceanic lidar systems, facilitating their deployment underwater. This is crucial conducting ocean observations that are free from interference at air-sea interface. However, simultaneous inversion volume scattering function 180° 532 nm (βm) and attenuation coefficient (