- Meat and Animal Product Quality
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Ruminant Nutrition and Digestive Physiology
- Identification and Quantification in Food
- Effects of Environmental Stressors on Livestock
- Calpain Protease Function and Regulation
- Advanced Chemical Sensor Technologies
- Spectroscopy and Chemometric Analyses
- Insect Utilization and Effects
- Reproductive Physiology in Livestock
- Bee Products Chemical Analysis
- Autophagy in Disease and Therapy
- Protein Hydrolysis and Bioactive Peptides
- Molecular Biology Techniques and Applications
- Muscle Physiology and Disorders
- Agricultural and Rural Development Research
- Animal Behavior and Welfare Studies
- Metabolomics and Mass Spectrometry Studies
- Probiotics and Fermented Foods
- Rangeland Management and Livestock Ecology
- Muscle metabolism and nutrition
- Agroforestry and silvopastoral systems
- Infant Nutrition and Health
- Animal health and immunology
Instituto de Investigación Sanitaria del Principado de Asturias
2018-2024
Servicio Regional de Investigación y Desarrollo Agroalimentario
2013-2024
Universidad de Oviedo
1997
Instituto de Productos Lácteos de Asturias
1995
Different bovine breeds and production systems are used worldwide, giving rise to differences in intrinsic extrinsic characteristics of beef. In order meet the consumer requirements, new approaches currently being developed guarantee tenderness, taste, juiciness However, final perception is complex, it also affected by several interrelated variables. This study aimed evaluate physicochemical parameters sensory profile three Spanish cattle under different livestock (extensive intensive)...
The solvent extraction of alkanes from faeces and herbage samples at two different temperatures (cold: 15–25°C hot: 65°C) was studied in four matrix types (cattle faeces, sheep hill grass heather), experiments performed Villaviciosa, Asturias, Spain 1994. Two internal standards (IS) chain length (C 22 C 34 ) were used to estimate alkane concentrations. Significant differences detected derived temperature extraction, IS sample matrix. At the cold temperature, long-chain not complete,...
Abstract The effect of n ‐alkane faecal recovery on the accuracy diet composition estimates, when increasing number components, was studied. Seven dietary treatments, composed different proportions herbaceous ( Lolium perenne and Trifolium repens ) woody species Erica umbellata, cinerea, Calluna vulgaris, arborea Ulex gallii were offered to 28 goats housed in metabolism crates. Diet estimated from alkane concentrations faeces, with without correction for incomplete recoveries, using...
Aging is characterized by a progressive loss of skeletal muscle mass and function (sarcopenia). Obesity exacerbates age-related decline lead to frailty. Skeletal fat infiltration increases with aging seems be crucial for the progression sarcopenia. Additionally, plasticity modulates metabolic adaptation different pathophysiological situations. Thus, cellular bioenergetics mitochondrial profile were studied in overweight aged people without reaching obesity prevent this extreme situation....
The conversion of muscle into meat is a complex process major concern for scientists due to its influence on the final quality. aim this study was investigate occurrence autophagic processes in meat. Our findings demonstrated, first time, tissue at early postmortem period (2 h 24 h) both beef breeds studied (Asturiana de los Valles and Asturiana la Montaña) showing significant time-scale differences between breeds, which could indicate role maturation. These have different physiological...
Abstract BACKGROUND: The objective of this work was to study the post‐mortem evolution potential biomarkers (µ‐calpain activity and proteolytic profile) meat tenderisation in bovine longissimus dorsi (LD) muscle from several biotypes coming two beef breeds (‘Asturiana de los Valles’ ‘Asturiana la Montaña’) showing different levels muscular hypertrophy ( mh / mh, /+, + /+). RESULTS: LD samples were taken at 2, 12, 24 48 h 3, 7, 14 21 days post‐mortem. presence produced a faster rate pH...
Influence of ultimate pH (pHu) on the occurrence defective meats known as dark, firm, and dry (DFD) has been studied through a proteomic approach at early post-mortem times. The myofibrillar sub-proteome longissimus thoracis et lumborum muscle from twelve loin samples Asturiana de los Valles x Friesian yearling bulls, previously classified into two groups six according to their values (normal, pHu < 6.0 high, ≥ 6.0), is analyzed 24 h post-mortem. Fractionation/enrichment carried out by...
This study investigated the effect of different cattle management strategies at farm (Intensive vs. Extensive) and during transport lairage (mixing non-mixing with unfamiliar animals) on myofibrillar subproteome
In an animal production system, different stressors may cause the depletion of muscle glycogen stores, resulting in elevated pH at 24 h post mortem (pH24), which leads to cell metabolism alterations that affect conversion into meat, causing meat quality defects, such as dark-cutting beef, also known dark, firm, and dry (DFD) beef. This process involve alteration small non-coding RNAs (miRNAs), play critical regulatory roles cellular processes. Here, we determined whether differential miRNA...
Abstract The live-weight responses, voluntary herbage intakes and diet compositions of two breeds sheep different mature live weights, grazing contrasting hill vegetation communities, were studied over three consecutive seasons. A total 228 Latxa 255 Gallega non-lactating ewes, with weights 42.4 33.1 kg respectively, grazed replicates, each 5-ha plots comprising by area either 0⋅3 Calluna vulgaris 0.7 grass species ( Agrostis capillaris, Festuca rubra Nardus stricta ) (treatment C 3 or C....
The objective of this work was to study the postmortem evolution potential biomarkers autophagy (Beclin 1, LC3-II/LC3-I ratio) and oxidative stress (total antioxidant activity, TAA; superoxide dismutase SOD catalase CAT) in Longissimus dorsi muscle entire male ((Large White×Landrace)×Duroc) pigs subjected different management treatments that may promote stress, such as mixing unfamiliar animals at farm and/or during transport lairage before slaughter. During rearing period farm, five were...
Seventy-eight calves from Asturiana de los Valles, Retinta, and Rubia Gallega breeds, under extensive intensive farm systems animal mixing non-mixing conditions, during the transport lairage in slaughterhouses, were studied. This research aimed to study effect of breed, system conditions on serum biomarkers (cortisol, lactate, glucose, amyloid A, haptoglobin, C-reactive protein) their relationship with pHu at slaughter time, evaluate response throughout fattening period. Moreover, this aims...
Defects in meat quality such as dark, firm and dry (DFD) beef have been related to high levels of oxidative stress that produce cellular alterations may affect the process acquisition. Despite important role endoplasmic reticulum (ER) response stress, its function muscle-to-meat conversion has not yet studied. In this study, differences muscular antioxidant defense unfolded protein (UPR) ER CONTROL (normal pH24) firm, (DFD, pH24 ≥ 6.2) at 24 h post-mortem were analyzed understand changes...