A5090099780- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Pharmacogenetics and Drug Metabolism
- Protein Structure and Dynamics
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Enzyme function and inhibition
- Antibiotics Pharmacokinetics and Efficacy
- Cancer Risks and Factors
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- Lignin and Wood Chemistry
- Pain Management and Opioid Use
- Chromosomal and Genetic Variations
- Analytical Methods in Pharmaceuticals
- Cancer therapeutics and mechanisms
- Molecular Biology Techniques and Applications
- Galectins and Cancer Biology
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Pharmaceutical studies and practices
- NF-κB Signaling Pathways
- Catalysis for Biomass Conversion
- Cell death mechanisms and regulation
Southern University of Science and Technology
2025
National Cancer Institute
2021-2023
National Institutes of Health
2023
University of Louisville
2016-2019
Second Xiangya Hospital of Central South University
2016
Central South University
2016
Canada's Michael Smith Genome Sciences Centre
2015
University of Minnesota
2010-2013
Biotechnology Institute
2011-2013
University of Maryland, Baltimore
1998
New protein parameters are reported for the all-atom empirical energy function in CHARMM program. The parameter evaluation was based on a self-consistent approach designed to achieve balance between internal (bonding) and interaction (nonbonding) terms of force field among solvent-solvent, solvent-solute, solute-solute interactions. Optimization used experimental gas-phase geometries, vibrational spectra, torsional surfaces supplemented with ab initio results. peptide backbone bonding were...
Alcohol oxidases, including carbohydrate have a long history of research that has generated fundamental biological understanding and biotechnological applications. Despite study, the galactose 6-oxidase/glyoxal oxidase family mononuclear copper-radical Auxiliary Activity Family 5 (AA5), is currently represented by only very few characterized members. Here we report recombinant production detailed structure–function analyses two homologues from phytopathogenic fungi Colletotrichum graminicola...
Aromatic allylamines are essential in the synthesis of diverse pharmaceutical building blocks. While oxidative allylic C–H amination using anilines has advanced significantly, achieving intermolecular reductive olefins with readily available nitroarenes remains a considerable challenge. Here, we demonstrate that replacing silanes combination protons and electrons enables selective amination, avoiding competing hydroamination pathway. The key to this transformation is use...
Aromatic allylamines are essential in the synthesis of diverse pharmaceutical building blocks. While oxidative allylic C–H amination using anilines has advanced significantly, achieving intermolecular reductive olefins with readily available nitroarenes remains a considerable challenge. Here, we demonstrate that replacing silanes combination protons and electrons enables selective amination, avoiding competing hydroamination pathway. The key to this transformation is use...
Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in alcohol binding pocket, L29P, Pseudomonas fluorescens (SIK WI) aryl esterase (PFE) increased specificity constant PFE for peracetic >100-fold [Bernhardt et al. (2005) Angew. Chem., Int. Ed. 44, 2742]. In this paper, extend work to address three following questions. First, what is molecular basis increase...
Abstract Background NEO201 is a humanized IgG1 monoclonal antibody (mAb) generated against tumor-associated antigens from patients with colorectal cancer. NEO-201 binds to core 1 or extended O-glycans expressed by its target cells. Here, we present outcomes phase I trial of in advanced solid tumors that have not responded standard treatments. Methods This was single site, open label 3 + dose escalation clinical trial. administered intravenously every two weeks 28-day cycle at level (DL) (1...
A 31-year-old Gravida 2 Para 1 Hispanic female with a history of classic Hodgkin lymphoma (cHL) presented to an outside hospital fatigue, normocytic–normochromic anemia (hemoglobin 6.0 g/dL), and severe abdominal lower back pain at 11 weeks gestation. Ultrasound showed large mesenteric retroperitoneal masses measuring up 10 cm in diameter. Biopsy 13 weeks' gestation documented nodular sclerosis cHL. The patient had received 6 cycles doxorubicin, bleomycin, vinblastine, dacarbazine (known as...
Abstract Lipase from Candida rugosa shows high enantioselectivity toward α‐substituted chiral acids such as 2‐arylpropionic acids. To understand how lipase (CRL) distinguishes between enantiomers of acids, we determined the X‐ray crystal structure a transition‐state analog covalently linked to CRL. CRL moderate ( E =23) methyl 2‐methoxy‐2‐phenylacetate, 1 ‐methyl ester, favoring S )‐enantiomer. We synthesized phosphonate R C , P )‐ 3 which, upon reaction with CRL, mimics transition state for...
Abstract Several serine hydrolases catalyze a promiscuous reaction: perhydrolysis of carboxylic acids to form peroxycarboxylic acids. The working hypothesis is that perhydrolases are more selective than esterases for hydrogen peroxide over water. In this study, we tested hypothesis, and focused on L29P‐PFE ( Pseudomonas fluorescens esterase), which catalyzes acetic acid 43‐fold faster wild‐type PFE. This predicts should be approximately PFE, but experiments show less selective. ratio...
Aim of presented study was to investigate whether EGCG could sensitize TRAIL resistant NPC cells TRAIL-mediated apoptosis. Three human cell-lines CNE-1, CNE-2, C666-1 and a non-transformed nasopharyngeal epithelium cell-line NP-69 were treated with or/and TRAIL. The apoptosis receptors determined by flow cytometric analysis. protein expression western blotting. Mitochondrial transmembrane potential dertemined DiOC6 (3). the only one that selected be EGCG. ECGC Combinatorial treatments led...
Abstract Some serine hydrolases also catalyze a promiscuous reaction— reversible perhydrolysis of carboxylic acids to make peroxycarboxylic acids. Five X‐ray crystal structures these acid perhydrolases show proline in the oxyanion loop. Here, we test whether this is essential for high activity using Pseudomonas fluorescens esterase (PFE). The L29P variant catalyzes 43‐fold faster ( k cat comparison) than wild type. Surprisingly, saturation mutagenesis at 29 position PFE identified six other...
Genetic polymorphisms of cytochrome P450 are contributors to variability in individual response drugs. Within the family, CYP2D6 is responsible for metabolizing hydrocodone, a widely prescribed opioid pain management. Alternatively, CYP3A4 and CYP3A5 can form norhydrocodone dihydrocodeine. We have previously found that postcesarean section cohort, rate hydromorphone formation was dependent on genotype plasma hydromorphone, not predictive relief.Blood obtained from cohort were surveyed common...