A5020707495- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- HER2/EGFR in Cancer Research
- Medical Imaging Techniques and Applications
- Peptidase Inhibition and Analysis
- Medical Imaging and Pathology Studies
- Education Pedagogy and Practices
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Drug Transport and Resistance Mechanisms
- Protein purification and stability
- Vitamin D Research Studies
- Environmental Sustainability and Education
- RNA Interference and Gene Delivery
- Rural and Ethnic Education
- History of Education Research in Brazil
- Education and Public Policy
- ATP Synthase and ATPases Research
- Toxin Mechanisms and Immunotoxins
- Stress and Burnout Research
- Lanthanide and Transition Metal Complexes
- Education during COVID-19 pandemic
- Head and Neck Cancer Studies
- DNA and Nucleic Acid Chemistry
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2024
Universidade do Porto
2024
UCLouvain
2024
Centro Hospitalar Lisboa Norte
2023
University College London
2015-2021
The Gordon Hospital
2021
University College Lahore
2017-2021
King's College London
2017-2018
St Thomas' Hospital
2017-2018
Instituto Superior Técnico
2012-2017
Studies examining whether vitamin D supplementation increases muscle mass or muscle-specific receptor (VDR) concentration are lacking. Our objective was to determine D3 4000 IU/d alters fiber cross-sectional area (FCSA) and intramyonuclear VDR over 4 months. This a randomized, double-blind, placebo-controlled study in single center. Participants were 21 mobility-limited women (aged ≥65 years) with serum 25-hydroxyvitamin (25OHD) levels of 22.5 60 nmol/L. Baseline 4-month FCSA measured from...
A next generation maleimide–ADC is shown to have excellent stability in blood serum, as well high potency and selectivity <italic>in vitro</italic>.
One of the most fundamental adaptive physiological events is response skeletal muscle to high-intensity resistance exercise, resulting in increased protein synthesis and ultimately larger mass. However, growth contraction attenuated older humans. Impaired contractile-induced may contribute sarcopenia: age-associated loss mass function that manifested by strength, contractile capacity, endurance. We hypothesized storage ceramide would be individuals this associated with increases NFκB...
Disulfide bridging offers a convenient approach to generate site-selective antibody conjugates from native antibodies. To optimise the reagents available achieve this strategy, we describe here use of dibromomaleimides designed undergo accelerated post-conjugation hydrolysis. Conjugation and hydrolysis, which serve 'lock' as robustly stable maleamic acids, is achieved in just over 1 h. This dramatic acceleration also shown infer significant improvements homogeneity, demonstrated by mass...
Thiol-stable albumin biologics are enabled by controlled, quantitative hydrolysis of maleimide–albumin conjugates, <italic>i.e.</italic> with no retro-Michael.
Conjugation of next generation maleimides to engineered cysteines in a THIOMAB™ antibody delivers highly stable and potent antibody-drug conjugate.
We describe investigations to expand the scope of next generation maleimide cross-linkers for construction homogeneous protein–protein conjugates. Diiodomaleimides are shown offer ideal properties rapid bioconjugation with reduced hydrolysis, allowing cross-linking even sterically hindered systems. The optimized linkers exploited link human serum albumin antibody fragments (Fab or scFv) as a prospective half-life extension platform, retention antigen binding and robust stability. Finally,...
Este artigo apresenta um relato das atividades realizadas no Programa Alfabetiza Pará, com ênfase na exploração do gênero textual parlendas, direcionado aos alunos 2º ano Ensino Fundamental da Escola Municipal de São Sebastião, sob a orientação professora Graciane Castro Taveira, licenciada em Letras e especialista neuropsicopedagogia Educação Inclusiva. A rotina foi estruturada três dias, englobando práticas leitura, oralidade alfabetização, alinhadas às competências habilidades previstas...
The delivery of therapeutic molecules to the central nervous system is hampered by poor across blood-brain barrier (BBB). Several strategies have been proposed enhance transport into brain, including invasive techniques and receptor-mediated (RMT). Both approaches several drawbacks, such as BBB disruption, receptor saturation, off-target effects, raising safety issues. Herein, we show that specific domains Dengue virus type 2 capsid protein (DEN2C) can be used trans-BBB peptide vectors....
Despite being widely used in the clinical setting for sentinel lymph node detection (SLND), (99m)Tc-based colloids (e.g., (99m)Tc-human serum albumin colloids) present a set of properties that are far from ideal. Aiming to design novel compounds with improved biological properties, we describe herein first class fully characterized (99m)Tc(CO)₃-mannosylated dextran derivatives adequate features SLND. Dextran derivatives, containing same number pendant mannose units (13) and variable (n)...
The aim of the present study is to synthesize new mannosylated dextran derivative that can be labeled with Tc-99m for potential use in sentinel lymph node detection (SLND). compound was designed have a molecular weight 10 kDa as backbone, mannose binding receptors and S-derivatized cysteine suitable chelator labeling [(99m)Tc(H(2)O)(3)(CO)(3)](+) precursor. Reaction allyl bromide (MW 11800) yielded intermediate allyl-dextran (1) about 40% coupling. Addition resulted cysteine, DC15 (2). final...
Aiming at the design of specific melanocortin-1 receptor (MC1R) targeted imaging probes, we report on effect different azolyl-ring substitution patterns (carboxylate 4-position and/or methyl groups 3,5 positions) pyrazolyl-diamine bifunctional chelators (Pz(2)-Pz(4)) pharmacokinetic profile (99m)Tc(CO)3-labeled lactam bridge-cyclized α-melanocyte stimulating hormone derivative, βAlaNleCycMSH(hex). Three pyrazolyl-diamine-containing were conjugated to βAlaNleCycMSHhex, with resulting peptide...
Current methods for sentinel lymph node (SLN) mapping involve the use of radioactivity detection with technetium-99m sulfur colloid and/or visually guided identification using a blue dye. To overcome kinetic variations two individual imaging agents through lymphatic system, we report herein on multifunctional macromolecules, 5a and 6a, that contain radionuclide ((99m)Tc or (68)Ga) near-infrared (NIR) reporter pre- intraoperative SLN by nuclear NIR optical techniques. Both bimodal probes are...
In order for platinum complexes to target cancer cells, trans ‐Pt II or IV were bioconjugated the cyclic peptide cRGDfK (cRGD), which has an affinity α v β 3 ‐integrin receptors, through its 4‐picolinic acid spectator ligands. To tackle this goal, Pt and precursors activated at their carboxylic functional group, futher treated with peptide, afford bioconjugates –cRGD –cRGD, respectively. was studied by 195 NMR spectroscopy, confirmed presence of center. contrast, characterization not...
Aiming to design 99m Tc complexes for probing nitric oxide synthase ( NOS ) by SPECT , we synthesized conjugates L4 – L6 comprising a ‐recognizing moiety connected diamino‐propionic acid (dap) chelating unit. The led of the type fac ‐[M( CO 3 (ĸ ‐L)] (M = Re/ Tc; Re4 / Tc4 : L ; Re5 Tc5 L5 Re6 Tc6 ). Enzymatic studies showed that and but not gave are less potent than conjugates. To rationalize these results, performed docking molecular dynamics simulations. high affinity is due strong...
The melanocortin receptor 1 (MC1R) is a specific molecular target for detection and therapy of melanoma, as it overexpressed in human melanomas. design novel peptide-based MC1R-specific probes imaging or targeted radionuclide being actively pursued. Aiming to visualize melanoma vivo by single photon emission computed tomography (SPECT) using 99mTc(CO)3-labeled cyclic melanocyte stimulating hormone analogues, we have designed the peptides c[S-NO2-C6H-CO-His-DPhe-Arg-Trp-Cys]-Lys-NH2 (1)...