A5028663907- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Food Safety and Hygiene
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Plant Pathogens and Fungal Diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Environmental DNA in Biodiversity Studies
- Plant-Microbe Interactions and Immunity
- Aquaculture disease management and microbiota
- Essential Oils and Antimicrobial Activity
- Plant biochemistry and biosynthesis
- Enterobacteriaceae and Cronobacter Research
- Psidium guajava Extracts and Applications
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Marine Bivalve and Aquaculture Studies
- Indian and Buddhist Studies
- Mycotoxins in Agriculture and Food
United States Food and Drug Administration
2015-2025
Center for Food Safety and Applied Nutrition
2015-2024
The University of Adelaide
2024
Center for Veterinary Medicine
2021
Food and Drug Administration
2015-2020
Oak Ridge National Laboratory
2008
Massachusetts General Hospital
2008
University of California, Davis
2008
Microbiota that co-enrich during efforts to recover pathogens from foodborne outbreaks interfere with efficient detection and recovery. Here, dynamics of co-enriching microbiota recovery Listeria monocytogenes naturally contaminated ice cream samples linked an outbreak are described for three different initial enrichment formulations used by the Food Drug Administration (FDA), International Organization Standardization (ISO), United States Department Agriculture (USDA). Enrichment cultures...
Next Generation Sequencing and the application of metagenomic analyses can be used to answer questions about animal diet choice study consequences selective foraging by herbivores. The quantification herbivore with respect native versus exotic plant species is particularly relevant given concerns invasive establishment their effects on ecosystems. While increased abundance white-tailed deer (
The microbiome of cheese is diverse, even within a variety. metagenomics dependent on vast array biotic and abiotic factors. Biotic factors include the population microbiota their resulting cellular metabolism. Abiotic factors, including pH, water activity, fat, salt, moisture content matrix, as well environmental conditions (temperature, humidity, location aging), influence This study assessed commercial Gouda prepared using pasteurized or unpasteurized cow milk goat via 16S rDNA...
An important data gap in our understanding of the phyllosphere surrounds origin many microbes described as communities. Most sampling research has focused on collection microbiota without use a control, so opportunity to determine which taxa are actually driven by biology and physiology plants opposed introduced environmental forces yet be fully realized. To address this gap, we used plastic inanimate controls adjacent live tomato (phyllosphere) field with hope distinguishing between...
ABSTRACT Vibrio parahaemolyticus is an important human foodborne pathogen whose transmission associated with the consumption of contaminated seafood, a growing number infections reported over recent years worldwide. A multilocus sequence typing (MLST) database for V. was created in 2008, and large clones have been identified, causing severe outbreaks worldwide (sequence type 3 [ST3]), recurrent certain regions (e.g., ST36), or spreading to other where they are nonendemic ST88 ST189). The...
Microgreens, like sprouts, are relatively fast-growing products and generally consumed raw. Moreover, as observed for microbial contamination from preharvest sources may also be present in the production of microgreens. In this study, two Salmonella enterica serovars (Hartford Cubana), applied at multiple inoculation levels, were evaluated survival growth on alfalfa sprouts Swiss chard microgreens by using most-probable-number (MPN) method. Various abiotic factors examined their effects...
The Eastern Shore of Virginia (ESV) is a major agricultural region in and the past has been linked to some tomato-associated outbreaks salmonellosis. In this study, water samples were collected weekly from irrigation ponds wells four representative vegetable farms (Farms A-D, each farm paired with one pond well) creek as well. addition, two sites Chesapeake Bay on ESV monthly. Poultry litter was sampled monthly three commercial broiler farms. Soil after fertilization poultry 10 2014, another...
Abstract Background The more quickly bacterial pathogens responsible for foodborne illness outbreaks can be linked to a vehicle of transmission or source, the illnesses prevented. Whole genome sequencing (WGS) based approaches source tracking have greatly increased speed and resolution with which public health response pinpoint outbreaks. Traditionally, WGS focused on culture an individual isolate before proceeding DNA extraction sequencing. For Listeria monocytogenes (Lm), generation...
Outbreaks of foodborne illnesses linked to fresh fruits and vegetables have been key drivers behind a wide breadth research aiming fill data gaps in our understanding the total ecology agricultural water sources such as ponds wells relationship this pathogens Salmonella enterica Listeria monocytogenes . Both S. L. can persist irrigation produce contamination events. Data describing abundance these organisms specific are valuable guide treatment measures. Here, we profiled culture independent...
Abstract Prepared and stored feeds, fodder, silage, hay may be contaminated by toxic plants resulting in the loss of livestock. Several poisonous have played significant roles livestock deaths from forage consumption recent years Western United States including Salvia reflexa . Metagenomic data, genome skims metabarcodes, been used for identification characterization complex matrices diet composition animals, mixed forages, herbal products. Here, chemistry, skims, metabarcoding were to...
Freshwater can support the survival of enteric pathogen
Foodborne outbreaks associated with Shiga toxin-producing Escherichia coli (STEC) contaminated wheat flour have been an increasing food safety concern in recent decades. However, there is little literature aimed at investigating the impact of different types on persistence STEC during storage and thermal inactivation. Therefore, two serovars STEC, O121 O157, were selected to inoculate each five common flours: whole wheat, bleached, unbleached, bread, self-rising. Inoculated flours examined...
Introduction Identification of chemical toxins from complex or highly processed foods can present ‘needle in the haystack’ challenges for chemists. Metagenomic data be used to guide toxicity evaluations by providing DNA-based description wholistic composition (eukaryotic, bacterial, protozoal, viral, and antimicrobial resistance) suspected harbor toxins, allergens, pathogens. This type information focus chemistry-based diagnostics, improve hazard characterization risk assessment, address...
Dingoes are culturally and ecologically important free-living canids whose ancestors arrived in Australia over 3,000 B.P., likely transported by seafaring people. However, the early history of dingoes Australia—including number founding populations their routes introduction—remains uncertain. This uncertainty arises partly from complex poorly understood relationship between modern New Guinea singing dogs, suspicions that post-Colonial hybridization has introduced recent domestic dog ancestry...
Salmonella enterica subsp. serovar Newport has been associated with various foodborne outbreaks in humans and animals. Phylogenetically, is one of several serovars that are polyphyletic. To understand more about the polyphyletic nature this serovar, six food, environment, human isolates from different lineages were selected for genome comparison analyses. Whole comparisons demonstrated heterogeneity mostly occurred prophage regions. Lineage-specific characteristics also present pathogenicity...
Consumption of cucumbers (Cucumis sativus var. sativus) has been linked to several foodborne outbreaks involving Salmonella enterica. The purpose this work was investigate the efficiency colonization and internalization S. enterica into cucumber plants by various routes contamination. Produce-associated outbreak strains (a cocktail serovars Javiana, Montevideo, Newport, Poona, Typhimurium) were introduced three cultivars (two slicing one pickling) via blossoms (ca. 6.4 log10 CFU/blossom, 4.5...
Salmonella enterica can survive in surface waters (SuWa), and the role of nonhost environments its transmission has acquired increasing relevance. In this study, we conducted comparative genomic analyses 172 S. isolates collected from SuWa across 3 months six states central Mexico during 2019. dynamics were assessed using 87 experimental 112 public 2002 through We also studied genetic relatedness between human clinical strains North America 2005 2020. Among isolates, identified 41 serovars...
Describing baseline microbiota associated with agricultural commodities in the field is an important step towards improving our understanding of a wide range objectives from plant pathology and horticultural sustainability, to food safety. Environmental pressures on plants (wind, dust, drought, water, temperature) vary by geography characterizing impact these variable phyllosphere will contribute improved stewardship fresh produce for both human health. A higher resolution incidence...
Milk-Borne Streptococcic Infections Ernest L. Stebbins, Hollis S. Ingraham, and Elizabeth A. Reed CopyRight*Read before the Epidemiology Section of American Public Health Association at Sixty-sixth Annual Meeting in New York, N. Y., October 6, 1937. https://doi.org/10.2105/AJPH.27.12.1259 Published Online: August 29, 2011
The National Antimicrobial Resistance Monitoring System (NARMS) has monitored antimicrobial resistance (AMR) associated with pathogens of humans and animals since 1996. In alignment One Health strategic planning, NARMS is currently exploring the inclusion surface waters as an environmental modality for monitoring AMR. From a perspective, function key integrators between humans, animals, agriculture, environment. Surface however, due to their dilute nature present unique challenge critically...
Abstract Background Whole genome sequencing of cultured pathogens is the state art public health response for bioinformatic source tracking illness outbreaks. Quasimetagenomics can substantially reduce amount culturing needed before a high quality be recovered. Highly accurate short read data analyzed single nucleotide polymorphisms and multi-locus sequence types to differentiate strains but cannot span many genomic repeats, resulting in highly fragmented assemblies. Long reads much more...