A5014836221- Genetically Modified Organisms Research
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Insect symbiosis and bacterial influences
- Plant and animal studies
- Cerebral Palsy and Movement Disorders
- Graphene research and applications
- Animal Genetics and Reproduction
- Botulinum Toxin and Related Neurological Disorders
- Supercapacitor Materials and Fabrication
- Offshore Engineering and Technologies
- Advancements in Battery Materials
- Plant Genetic and Mutation Studies
- Structural Integrity and Reliability Analysis
- Stroke Rehabilitation and Recovery
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
Institute of Plant Protection
2020-2025
Jiangsu Academy of Agricultural Sciences
2020-2025
Nanjing Agricultural University
2018-2022
Ministry of Ecology and Environment
2018-2022
Guangzhou University of Chinese Medicine
2022
Nanjing Institute of Environmental Sciences
2018-2022
Beijing Normal University
2013
Southwest Petroleum University
2012
Vitellogenin (Vg) is a well-known nutritious protein involved in reproduction nearly all oviparous animals, including insects. Recently, Vg has been detected saliva proteomes of several piercing-sucking herbivorous arthropods, the small brown planthopper (Laodelphax striatellus, SBPH). Its function, however, remains unexplored. We investigated molecular mechanism underlying SBPH orally secreted Vg-mediated manipulation plant-insect interaction by RNA interference, phytohormone and H2 O2...
The small brown planthopper ( Laodelphax striatellus ; SBPH) is a piercing-sucking insect that secretes salivary proteins into its plant host during feeding. However, the mechanisms by which these regulate defense responses remain poorly understood. Here, we identified disulfide isomerase (LsPDI1) in SBPH proteome. LsPDI1 was highly expressed glands and secreted rice plants Transient planta expression absence of signal peptide induced reactive oxygen species (ROS) burst, cell death, callose...
Abstract Plants activate defense machinery when infested by herbivorous insects but avoid such costs in the absence of herbivory. However, key signaling pathway regulators underlying flexibility and mechanisms that exploit these components to disarm plant systems remain elusive. Here, it is reported immune repressor 14‐3‐3e rice Oryza sativa (OsGF14e) regulates homeostasis. Infestation with brown planthopper (BPH) Nilaparvata lugens decreased OsGF14e expression; however, level downregulation...
Abstract BACKGROUND Calcium (Ca 2+ )‐binding proteins in the saliva of herbivorous insects function as effectors to attenuate host plant defenses and thus improve insect feeding performance. Silencing these genes via transgenic plant‐mediated RNAi is a promising pest control strategy. However, their sequences functions small brown planthopper Laodelphax striatellus (SBPH) remain be investigated. RESULTS We identified putative EF‐hand Ca ‐binding protein (LsECP1) SBPH watery saliva. LsECP1...
The brown planthopper (Nilaparvata lugens, BPH) and small (Laodelphax striatellus, SBPH) are major pests of rice (Oryza sativa) in Asia. These piercing-sucking insects secrete saliva into the host during feeding. Nevertheless, it is largely unknown how planthoppers use salivary effectors to enable continuous feeding on rice. Here, we screened their proteomes selected eight proteins conserved between SBPH BPH as candidate effectors. Silencing calmodulin (CaM) impeded from penetrating phloem....
Exogenous genes of transgenic crops are usually transferred to their wild-type relatives through pollen-mediated gene flow, which may change the ecological fitness and ability invade wild populations, resulting in weeding plants other unpredictable environmental impacts. In this study, F 1 generation herbicide-resistant soybeans was obtained by artificial pollination, 2 seeds were self-crossing, parents generations tested. The foreign protein EPSPS expressed normally hybrid between soybeans;...
The environmental release and biosafety of transgenic Bt crops have attracted global attention. China has a large area saline-alkali land, ideal for conducting large-scale application production rice. Therefore an understanding the fitness rices in saline-alkaline soils ability to predict its long-term impact are important future sustainable use these crops. In present study, cry1Ab/c rice Huahui1, which national certificate was obtained, grown on simulated normal farmland without target...
China has a large area of saline-alkaline land that can be utilized for the cultivation transgenic rice. Therefore, growth and reproductive behavior rice are not only problem production needs to resolved, but also an important aspect environmental risk assessment saline alkali soil. In present study, insect-resistant cry1C* rice, T1C-19, was grown in farmland soils. The transcription translation exogenous cry1C*, vegetative fitness, such as plant height, tiller number, biomass, filled grain...
Current risk assessments of transgenic crops do not take into consideration whether exogenous proteins interact with endogenous and thereby induce unintended effects in the crops. Therefore, through protein interactions insect-resistant rice merit investigation. Here, a yeast two-hybrid assay was used to evaluate between Bacillus thuringiensis (Bt) protein-derived Cry1Ab/c insect resistance Huahui-1 its parental Minghui-63. The authenticity strongest 14 involved photosynthesis stress...
Fitness is one of the key parameters to evaluate effects transgenic plants on ecological environment. To risk Bt cotton growing in different habitats, we determined expression exogenous gene and fitness non-transgenic three habitats (farmland, grassland, shrub). We observed that protein farmland was significantly higher than natural habitat, when growth environment suitable, level showed a downward trend with advancement growth. There were no significant differences plant height, aboveground...
The cellular localization of exogenous proteins expressed in transgenic crops not only determines their stability, but also effects on crop growth and development, including under stressful conditions; however, the underlying molecular mechanisms remain unknown. Here, we determined distribution exogenously Cry1Ab/c protein insect-resistant rice Huahui-1 (HH1) cells through subcellular localization, immunohistochemistry, immunofluorescence, western blot analyses. Interaction between...
Transgene escape into natural ecosystems through seed spraying or transgene introgression may potentially cause environmental biosafety problems. In this study, we assessed the risk of insect-resistant transgenic rice entering farmland margins adjacent to farmland. Transgenic Cry1C* (T1C-19) was used study effects exogenous expression on vegetative and reproductive growth indices under different growing conditions using following four combined treatments land use weeds: uncultivated without...
Genetic modifications in rice, which resulted insect resistance, have been highly efficacious. However, they also induced undesirable secondary phenotypes, such as delayed flowering. The molecular mechanisms associated with these unwanted effects remain unclear. Here, we showed that the flowering time for insect-resistant transgenic cry1Ab/c rice Huahui-1 (HH1) was delayed, compared parental Minghui-63 (MH63), cultivated on farmland and saline–alkaline soils. In contrast, cry1C ^* cultivars...
The submarine pipelines are vulnerable to physical factors, chemical factors and biological then lead corrosion. destruction of the is larger more difficult repair than land-based pipelines. This paper focuses on analysis influence corrosion in sea mud, internal pipelines, seawater corrosion, atmospheric other With failure as top event, fault tree has been established. Through systematically qualitative structural importance, conclusion was reached how above five kinds status anti-corrosive...
Considering the anticipated commercial exploitation of insect-resistant transgenic rice and that planting area cultivated overlaps with wild rice, simulating an escape from farmlands exploring its fitness after entering semi-natural or natural ecosystems through uncontrolled seed dispersal gene flow are critical to understand resulting potential long-term environmental risks. The expression foreign Cry1Ab/c protein vegetative reproductive Huahui1 (HH1) parental-line Minghui63 (MH63) were...