A5102359479- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Advanced Breast Cancer Therapies
- Advanced Electron Microscopy Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Circular RNAs in diseases
- Plant Stress Responses and Tolerance
- Oil and Gas Production Techniques
- Plant and fungal interactions
- Remote Sensing in Agriculture
- CAR-T cell therapy research
- Lipid metabolism and disorders
- Bioinformatics and Genomic Networks
- Liver physiology and pathology
- Mineral Processing and Grinding
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Fungal Biology and Applications
- Cholesterol and Lipid Metabolism
- Cancer Mechanisms and Therapy
- Plant Molecular Biology Research
- Protein Tyrosine Phosphatases
- Tissue Engineering and Regenerative Medicine
Kunming University
2021-2024
University of California, Riverside
2024
Dalian Medical University
2024
First Affiliated Hospital of Dalian Medical University
2024
State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation
2024
Anhui Agricultural University
2024
University of Michigan–Ann Arbor
2023
Harbin Medical University
2020-2023
Wuhan Puai Hospital
2023
Nanjing Agricultural University
2023
<p>Aberrant 3’SS splicing profiles of SF3B1-mutated cell lines and primary CLL</p>
<p>RNAseqCNV analysis uncovers widespread CNV events in SF3B1 mutant cell lines</p>
<p>FACS sorting and RhAMP SNP screening yield isogenic SF3B1 mutant clones</p>
Abstract Background Various studies have shown that circular RNA (circRNA) plays a pivotal role in chronic obstructive pulmonary disease (COPD). We aimed to determine the of circRNA BBS9 COPD progression. Methods Real-time quantitative reverse transcription PCR (qRT-PCR) was performed levels and linkages BBS9, miRNA-103a-3p, BCL2L13 cigarette smoke extract (CSE)-treated human microvascular endothelial cells (HPMECs). The target binding sites miRNA-103a-3p were predicted using starBase...
Abstract Chlorophyll is a crucial physiological and biochemical indicator that impacts plant photosynthesis, accumulation of photosynthetic products, final yield. The measurement analysis chlorophyll content in plants can provide valuable insights into their nutritional status overall health. non-destructive efficient estimation relevant indicators using hyperspectral technology reliable method for collecting data on nutrient levels health during growth development. Fifty-three Carya...
Richter’s transformation (RT) is a progression of chronic lymphocytic leukemia (CLL) to aggressive lymphoma. MGA ( Max gene associated ), functional MYC suppressor, mutated at 3% in CLL and 36% RT. However, genetic models molecular mechanisms deletion that drive RT remain elusive. We established an mouse model by knockout Mga the Sf3b1 / Mdr using CRISPR-Cas9 determine role Murine cells exhibited mitochondrial aberrations with elevated oxidative phosphorylation (OXPHOS). Through RNA...
The cytochrome P450 (CYP) is a large and complex eukaryotic gene superfamily with enzymatic activities involved in several physiological regulatory processes. As an objective, in-silico genome-wide DNA methylation (5mC) analysis was performed rice (Oryza sativa cv. Zhonghua11), the epigenetic role of CYPs two abiotic stresses observed. Being stable representative mark, DNA-methylation alters expression under stressful environmental conditions. Rice plants salinity drought were analyzed...
Abstract SF3B1 is the most recurrently mutated RNA splicing gene in cancer. However, research of its pathogenic role has been hindered by a lack disease-relevant cell line models. Here, our study compared four genome engineering platforms to establish mutant lines: CRISPR-Cas9 editing, AAV homology-directed repair base editing (ABEmax, ABE8e), and prime (PE2, PE3, PE5max). We showed that via PE5max achieved efficient K700E across wide range lines. Our approach was further refined coupling...
<p><i>SF3B1</i> mutation induces LOY in both cell lines and CLL patient samples. <b>A,</b> DEG analysis of HG-3 <i>SF3B1</i> mutant vs. WT lines. Log<sub>2</sub>FC ≥1 ≤−1 adjusted <i>P</i> value <0.05 chosen as statistical cutoffs. <b>B,</b> Schematic depiction chromosome (top) Y (bottom) 12 with PCR targets loci indicated. <b>C,</b> genes genomic DNA <b>D,</b> Top, Timeline for the...
<p>Engineering SF3B1 mutation into CLL cell lines necessitates an additional enrichment marker</p>
<p>PE2 and PE3 outperform base editing in engineering the K700E mutation</p>
<p>Splicing and growth phenotypes of HG-3 MEC-1 SF3B1 mutant cell lines</p>
<p>Aberrant 3’SS splicing profiles of SF3B1-mutated cell lines and primary CLL</p>
<p>Off-targeting analyses for PRECIS engineering of SF3B1 K700E mutation</p>
<p>Engineering SF3B1 mutation into CLL cell lines necessitates an additional enrichment marker</p>
<p>FACS sorting and RhAMP SNP screening yield isogenic SF3B1 mutant clones</p>