A5074696131- Vector-borne infectious diseases
- Viral Infections and Vectors
- Antibiotic Resistance in Bacteria
- Mosquito-borne diseases and control
- Salmonella and Campylobacter epidemiology
- Reproductive tract infections research
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- SARS-CoV-2 and COVID-19 Research
- Parasitic Diseases Research and Treatment
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Vibrio bacteria research studies
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- Bartonella species infections research
- Metal complexes synthesis and properties
- Rabies epidemiology and control
- Bacterial Identification and Susceptibility Testing
Auburn University
2014-2024
Zhuzhou Central Hospital
2013-2024
Central South University
2022-2024
Huazhong Agricultural University
2007-2024
Zhengzhou Central Hospital
2023
Beijing Institute of Technology
2023
Guangxi University
2021-2022
Guangxi University of Chinese Medicine
2021-2022
Hefei National Center for Physical Sciences at Nanoscale
2022
University of Science and Technology of China
2022
Poultry and livestock are the most important reservoirs for pathogenic Escherichia coli use of antimicrobials in animal farming is considered factor promoting emergence, selection dissemination antimicrobial-resistant microorganisms. The aim our study was to investigate antimicrobial resistance E. isolated from food animals Jiangsu, China. disc diffusion method used determine susceptibility 18 agents 862 clinical isolates collected chickens, ducks, pigs, cows between 2004 2012. Overall, 94%...
Abstract Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of treatment multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes ( mcr-1 , mcr-2 mcr-3 ) in 213 anal 1,339 nasal swabs from pigs (n = 1,454) nine provinces China, 1,696 cloacal 1,647 oropharyngeal samples poultry 1,836) at live-bird markets 24 provinces. The prevalences (79.2%) geese (71.7%) significantly higher than chickens (31.8%), ducks (34.6%)...
Abstract To investigate the prevalence and diversity of Chlamydia spp. in domestic birds China, oral cloacal swabs healthy chickens, ducks, geese pigeons were collected nationwide from live-animal markets examined by 23 S rRNA gene FRET-PCR followed high-resolution melting curve analysis confirmatory sequencing. Overall, 26.2% (602/2,300) positive for five identified. While occasional detection C. suis muridarum poultry is reported here first time, predominant chlamydial agent was C ....
Flies have the capacity to transfer pathogens between different environments, acting as one of most important vectors human diseases worldwide. In this study, we trapped flies on a university campus and tested them for mobile resistance genes against colistin, last-resort antibiotic in medicine treating clinical infections caused by multidrug-resistant Gram-negative bacteria. Quantitative PCR assays developed showed that 34.1% Musca domestica (86/252) 51.1% Protophormia terraenovae (23/45)...
To provide more complete data on SARS-CoV-2 infections in dogs and cats the U.S., we conducted a serosurvey convenience serum samples from (n=1336) (n=956) collected 48 states of USA 2020. An ELISA targeting antibody against nucleocapsid identified eleven positive two doubtful cats, five dogs. A surrogate neutralization assay detecting antibodies blocking attachment spike protein to ACE2 was with three samples, one 463 randomly selected negative samples. These four were confirmed by virus...
Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods whole genome sequencing (WGS). Of the recovered from humans, animals environment Nigeria, 18.9% (110/583) were resistant at least one (meropenem, ertapenem, imipenem) 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of 2-32...
ABSTRACT In this study, a total of 323 Salmonella enterica strains were isolated from 3,566 rectal swab samples 51 poultry farms in seven regions 12 provinces China between 2006 and 2012. The prevalences sp. carriage 12.4% geese (66 positive/533 samples), 10.4% turkeys (32/309), 9.8% chickens (167/1,706), 6.8% ducks (41/601), 4.1% pigeons (17/417), respectively. These isolates belonged to 20 serovars, which the most frequent serovars S. serovar Gallinarum biovar Pullorum (herein, S ....
Background Although tick-borne diseases are important causes of morbidity and mortality in dogs tropical areas, there is little information on the agents causing these infections Caribbean. Methodology We used PCRs to test blood from a cross-section St Kitts for Ehrlichia (E.) canis, Babesia (B.) spp., Anaplasma (A.) spp. Hepatozoon (H.) Antibodies against E. canis A. phagocytophilum/platys were detected using commercial immunochromatography tests. Records examined retrospectively obtain...
Rickettsia felis is a recently described flea-borne spotted fever group that an emerging human pathogen. Although there information on the organism from around world, no in China. We used commercial ELISA to detect antibodies reactive against R. blood samples and developed PCR gltA of external parasites. found people (16%; 28/180), dogs (47%; 128/271) cats (21%; 19/90) positive PCRs with DNA (0.1%; 1/822), (0.8%; 8/1,059), mice (10%; 1/10), ticks (Rhipicephalus sanguineus; 10%; 15/146), lice...
Although many vector-borne agents are potential zoonoses and cause substantial morbidity mortality in dogs worldwide, there limited data on these organisms of China.Quantitative PCRs for were performed to investigate their prevalences convenience whole blood samples obtained from 1114 21 veterinary clinics a commercial dog breeding facility ten provinces China. In addition, the 146 Rhipicephalus sanguineus senso lato 37 Linognathus setosus collected facility.DNAs Babesia gibsoni B. vogeli...
Highly drug-resistant Salmonella enterica serovar Indiana became the most common in broilers with diarrhea China over course of this study (15% 2010 to 70% 2014). While S. isolates (87%, 384/440) were resistant 13 16 antibiotics tested, 89% non-S. (528/595) 0 6 antibiotics. Class 1 integrons and IncHI2-type plasmids detected all isolates, but only 39% 1% isolates.
Antimicrobial resistance against colistin has emerged worldwide threatening the efficacy of one last-resort antimicrobials used for treatment Enterobacteriaceae. To investigate presence recently identified genes (mcr-4, mcr-5) in China, we established PCRs to detect mcr-4 and mcr-5 on 213 anal 1,339 nasal swabs from apparently healthy pigs (n = 1,454) nine provinces, 1,696 cloacal 1,647 oropharyngeal samples poultry 1,836) at live-bird markets 24 provinces China. The prevalence swine (41.4%;...
Background: Antimicrobial resistance is rising globally at an alarming rate. While multiple active surveillance programs have been established to monitor the antimicrobial resistance, studies on environmental link spread are lacking. Methods: A total of 493 flies were trapped from a dairy unit, dog kennel, poultry farm, beef cattle urban trash facility and downtown area isolate Escherichia coli, Klebsiella pneumoniae Staphylococcus spp. for susceptibility testing molecular characterization....
Abstract The rapid, simultaneous, and accurate identification of multiple non‐nucleic acid targets in clinical or food samples at room temperature is essential for public health. Argonautes (Agos) are guided, programmable, target‐activated, next‐generation nucleic endonucleases that could realize one‐pot multiplexed detection using a single enzyme, which cannot be achieved with CRISPR/Cas. However, currently reported thermophilic Ago‐based multi‐detection sensors mainly employed the acids....
Polymerase chain reaction (PCR) is a widely used technique in gene expression analysis, diagnostics, and various molecular biology applications. However, the accuracy sensitivity of PCR can be compromised by primer–template mismatches, potentially leading to erroneous results. In this study, we strategically designed 111 combinations with varying numbers, types, locations mismatches meticulously assess their impact on qPCR performance while two distinctly different types DNA polymerases were...
Abstract Autophagy is a conserved cellular process that functions as first-line defense to restrict the growth of invading parasitic bacteria. As an intracellular pathogen, Salmonella (S) Typhimurium invades host cells through two Type III secretion systems (T3SS) and resides in -containing vacuole (SCV). When SCV membrane perforated ruptured by T3SS-1, small portion egresses from replicates rapidly nutrient-rich cytosol. Cytosolic those residing membrane-damaged are tagged ubiquitination...