A5035024524- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Myeloid Leukemia Research
- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Chronic Lymphocytic Leukemia Research
- Hepatitis C virus research
- Acute Lymphoblastic Leukemia research
- Inflammasome and immune disorders
- Mesenchymal stem cell research
- RNA Research and Splicing
- Intracerebral and Subarachnoid Hemorrhage Research
- Metastasis and carcinoma case studies
- Circular RNAs in diseases
- Viral Infections and Vectors
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
S. M. Kirov Military Medical Academy
2019-2024
ABSTRACT The aim of this study was to investigate cell source‐dependent nucleic acids repertoire diverse subpopulations plasma extracellular vesicles (EVs). Blood from nine healthy volunteers used for the analysis. Samples EVs were obtained by differential centrifugation plasma. application high‐sensitivity fluorescence‐activated sorting (hs‐FAVS) using fluorophore‐conjugated anti‐CD41‐FITC (Fluorescein isothiocyanate) and anti‐CD235a‐PE antibodies allowed isolation three EVs, namely CD41+...
To date, there is indisputable evidence of significant CTC heterogeneity in carcinomas, particular breast cancer. The CTCs manifested the key characteristics tumor cells related to metastatic progression - stemness and epithelial-mesenchymal (EMT) plasticity. It still not clear what markers can characterize phenomenon EMT plasticity range from epithelial mesenchymal phenotypes. In this article we examine manifestations prospective study included 39 patients with invasive carcinoma no special...
Assessment of viral load levels in various biological samples taken from the respiratory tract can be an indicator ongoing process active replication and may used to monitor severe infections. The study relationship between SARS-CoV-2 immunological laboratory parameters is important step search for clinical markers COVID-19. aim this research was quantify patients with COVID-19 identify relation-ship changes cellular component immune system. A examination carried out on 74 diagnosed...
The expression of non-coding RNAs varies greatly depending on the cell type and physiological state. Changes in affect regulatory properties these molecules. We investigated RNA-seq data from sorted blood cells healthy control donors severe COVID-19 patients. Our aim was to display a distinguishable piRNA differential portrait for each study behavior Overall (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, neutrophils) differs type. Four most abundant were expressed relatively...
Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types blood cells separated fluorescence-activated sorting severe COVID−19 patients healthy control donors. In addition examining the behavior piRNA SARS-CoV−2 infected patients, our aim was present a distinct differential portrait for each...
Severe COVID-19 alters the biochemical and morphological characteristics of blood cells in a wide variety ways. To date, however, vast majority research has been devoted to study leukocytes, while erythrocyte changes have received significantly less attention. The aim this was identify morphology abnormalities that occur COVID-19, compare number different poikilocyte types, measure sizes provide data on size dispersion. Red obtained from 6 control donors (800-2200 per donor) 5 patients...
Severe COVID-19 alters the biochemical and morphological characteristics of blood cells in a wide variety ways. To date, however, vast majority research has been devoted to study leukocytes, while erythrocyte changes have received significantly less attention. The purpose this was identify types that were unique COVID-19, compare number different poikilocyte types, measure sizes provide data on size dispersion. Red obtained from 6 control donors (800-2200 for each donor) 5 patients (800-1900...
Physiological condition and cell type have a major impact on non-coding RNA expression. These molecules' regulatory characteristics are impacted by changes in We examined RNA-seq data from sorted blood cells of severe COVID-19 patients as well healthy control donors. In addition to examining the behavior piRNA patients, our goals were present distinct differential expression portrait for each separate type. Depending cell, different (erythrocytes, monocytes, lymphocytes, eosinophils,...
Postischemic neuroinflammation is a critical pathophysiological process within the entire pattern of cerebral ischemia. It characterized by microglial and astroglial activation accompanied disturbances in innate adaptive immune response. The early damage blood-brain barrier (BBB) integrity brain autoantigens release into circulation, particular, neurospecific protein S100B. According to recent experimental data, activated autophagy associated with postischemic neuroinflammation, involved its...
tRNA-derived fragments function as markers additionally to playing the role key of signalling molecules in a number disorders. It is known that repertoire these differs greatly different cell types and varies depending on physiological condition. The aim our research was compare pattern tRF expression main blood determine how composition changes during COVID-19-induced cytokine storm. Erythrocytes, monocytes, lymphocytes, neutrophils, basophils, eosinophils from control donors patients with...
tRNA-derived fragments function as markers in addition to playing the key role of signalling molecules a number disorders. It is known that repertoire these differs greatly different cell types and varies depending on physiological condition. The aim our research was compare pattern tRF expression main blood determine how composition changes during COVID-19-induced cytokine storms. Erythrocytes, monocytes, lymphocytes, neutrophils, basophils eosinophils from control donors patients with...
The postischemic inflammatory response plays a significant role in the pathogenesis of acute ischemic stroke (IS). It has been established that IS is accompanied by aseptic inflammation, which induces activation costimulatory molecules process innate immunity to brain tissue damage. constantly progressive destruction neuronal antigens contributes an increase volume lesion. Evidence continues accumulate indicating important NLRP3-mediated inflammation IS. shown autophagy involved cascade Many...
Extracellular vesicles are a heterogeneous group of particles surrounded by phospholipid bilayer and having diameter from 30 nm to 5 microns. In recent years, the involvement extracellular in pathogenesis many diseases, as well possibility their use diagnosis therapy, has been actively studied. It is known that can carry nucleic acids, particular micro-RNA, mRNA, etc., what often leads participation regulation pathological physiological processes body, intercellular cooperation. The...
Despite continuous attempts to improve therapy, the outcomes of acute myeloid leukemia remain almost unchanged over last decades. Drugs made with a more complete understanding biology do not equal hopes for better prognosis. The best results are achieved only high-dose chemotherapy, which is possible limited number patients. High phenotypic and genotypic heterogeneity defines relevance develop personalized approaches including those based on determination individual drug sensitivity blast...
To date, there is no consensus explaining the relationship between varying concentrations of IFNγ and severity infection caused by SARS-CoV-2. The aim this article was to analyze formulate conclusions from selected studies publications, which, in sum, provide a potentially reasonable view on role COVID-19 pathogenesis. This highlights current data immunological which affects differentiation naive T helper cells, acting as polarizing factor. It activates major histocompatibility complex (MHC)...