A5024163991- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Higher Education Teaching and Evaluation
- Educational Innovations and Technology
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Retinal and Optic Conditions
- E-Learning and Knowledge Management
- Adenosine and Purinergic Signaling
- Second Language Learning and Teaching
- Ocular Diseases and Behçet’s Syndrome
- Educational Technology in Learning
- Retinoids in leukemia and cellular processes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinal Imaging and Analysis
- Innovations in Medical Education
- Translation Studies and Practices
- Higher Education and Sustainability
- Nicotinic Acetylcholine Receptors Study
- Education and Teacher Training
- melanin and skin pigmentation
- Coenzyme Q10 studies and effects
- Biochemical effects in animals
- Vasculitis and related conditions
University of Alicante
2014-2023
Instituto de Salud Carlos III
2022
Retinitis pigmentosa is a heterogeneous group of inherited neurodegenerative retinal disorders characterized by progressive peripheral vision loss and night difficulties, subsequently leading to central impairment. Chronic microglia activation associated with various diseases including retinitis pigmentosa. The objective this study was quantify in the retina P23H rats, an animal model pigmentosa, evaluate therapeutic effects TUDCA (tauroursodeoxycholic acid), which has been described as...
Retinal neurodegenerative diseases involve a scenario of inflammation and cell death that leads to morphological alterations visual impairment. Non-ocular inflammatory processes could affect retinal disorders their progression, at least in part by activating microglial cells releasing pro-inflammatory cytokines. Our purpose was study the consequences systemic process progression degeneration P23H rats, retinitis pigmentosa (RP) model. In order induce mild chronic inflammation, we...
Microglia act as the resident immune cells of central nervous system, including retina. In response to damaging stimuli microglia adopt an activated state, which can progress into a phagocytic phenotype and play potentially harmful role by eliciting expression release pro-inflammatory cytokines. The aim present study was assess longitudinal changes in during retinal degeneration homozygous P23H rat, model dominant retinitis pigmentosa. Microglial phenotypes, morphology density were analyzed...
Significance The retina is considered a window to the brain, and retinal degenerative diseases involve same mechanisms as those of other neurodegenerative disorders. Neuronal degeneration complex process involving environmental stress, which can affect vulnerable neurons. High-fat diet–induced metabolic alterations may influence homeostasis exacerbate diseases. This study provides evidence that short-term high-fat feeding promotes glucose intolerance, gut microbiome dysbiosis, oxidative...
We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, be potentially harmful for patients with degenerative diseases.Activation was measured in a model sublethal invasive candidiasis C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, anti-CD45 antibodies.Systemic causes microglia, phenotypic changes morphology, surface markers expression, microglial relocation layers.As an...
Background/Aims: It is well established that oxidative stress and inflammation are common pathogenic features of retinal degenerative diseases.ITH12674 a novel compound induces the transcription factor Nrf2; in so doing, molecule exhibits anti-inflammatory, antioxidant properties, affords neuroprotection rat cortical neurons subjected to stress.We here tested hypothesis ITH12674 could slow degeneration causes blindness rd10 mice, model retinitis pigmentosa.Methods: Animals were...
Different ocular alterations have been described in patients with coronavirus disease 2019 (COVID-19). Our aim was to determine whether COVID-19 affected retinal cells and establish correlations clinical parameters.Retinal sections flat-mount retinas from human donors (n = 16) controls 15) were immunostained. The location of angiotensin-converting enzyme 2 (ACE2) the morphology microglial cells, Müller astrocytes, photoreceptors analyzed by confocal microscopy. Microglial quantification area...
Purpose: To assess the changes in retinal morphology a rat model of chronic glaucoma induced by ocular hypertension. Methods: Intraocular pressure (IOP) was surgically increased through weekly injections sodium hyaluronate (HYA) anterior eye chamber left male Wistar rats, whereas right eyes were sham operated (salt solution). During 10-week experimental period, IOP measured with rebound tonometer. Retinal cryosections prepared for histological/immunohistochemical analysis and morphometry....
The innate immune Toll-like receptor (TLR) family plays essential roles in cell proliferation, survival and function of the central nervous system. However, way which TLRs contribute to development maintenance proper retinal structure remains uncertain. In this work, we assess effect genetic TLR4 deletion on morphology retina mice. Visual acuity responsiveness were evaluated knockout wild type C57BL/6J control mice by means an optomotor test electroretinography, respectively, from P20 P360....
Although its actual role in the progression of degenerative processes is not fully known, persistent activated state retinal microglia and concurrent secretion inflammatory mediators may contribute to neuronal death permanent vision loss. Our objective was determine whether non-ocular conditions (immunosuppression peripheral inflammation) could lead activation microglia. Mouse models immunosuppression induced by cyclophosphamide and/or inflammation chemically sublethal colitis C57BL/6J mice...
The purinergic receptor P2X7 (P2X7R) is implicated in all neurodegenerative diseases of the central nervous system. It also involved retinal degeneration associated with glaucoma, age-related macular degeneration, and diabetic retinopathy, its overexpression retina evident these disorders. Retinitis pigmentosa a progressive degenerative disease that ultimately leads to blindness. Here, we investigated expression P2X7R during progression rd10 mouse model RP. As P2X4 widely co-expressed P2X7R,...