A5037447732- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Computability, Logic, AI Algorithms
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Advanced Mathematical Theories
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
- Endoplasmic Reticulum Stress and Disease
- Mathematics and Applications
- RNA regulation and disease
- Molecular Biology Techniques and Applications
- Mosquito-borne diseases and control
Iowa State University
2016-2023
Birla Institute of Technology, Mesra
2014
MicroRNAs are endogenous small noncoding RNAs which play critical roles in gene regulation. Few wheat (Triticum aestivum L.) miRNA sequences available miRBase repertoire and knowledge of their biological functions related to biotic stress is limited. We identified 52 miRNAs, belonging 19 families, from next-generation transcriptome sequence data based on homology search. One specific novel was but could not be ascribed or assigned any known family. Differentially expressed 22 miRNAs were...
Abstract The accumulation of misfolded proteins in the endoplasmic reticulum (ER) defines a condition called ER stress that induces unfolded protein response (UPR). UPR mammalian cells attenuates synthesis initiation, which prevents piling up ER. Mammalian rely on Protein Kinase RNA‐Like Endoplasmic Reticulum (PERK) phosphorylation eIF2α to arrest synthesis, however, plants do not have PERK homolog, so question is whether control translation stress. We compared changes RNA levels...
RNAs that are 5′-truncated versions of a longer RNA but share the same 3′ terminus can be generated by alternative promoters in transcription cellular mRNAs or replicating viruses. These truncated cannot distinguished from simple two-primer RT-PCR because primers anneal to cDNA smaller also to—and amplify—the RNA-derived cDNA. Thus, laborious methods, such as northern blot hybridization, used distinguish shorter RNAs. For rapid, low-cost, and specific detection these RNAs, we report d...
In recent years, a new class of viral noncoding subgenomic RNA (ncsgRNA) has been identified. This is generated as stable degradation product via an exoribonuclease-resistant (xrRNA) structure, which blocks the progression 5'→3' exoribonuclease on RNAs in infected cells. Here, we assess effects ncsgRNA red clover necrotic mosaic virus (RCNMV), called SR1f, plants. We demonstrate following: (i) absence SR1f reduces symptoms and decreases accumulation Nicotiana benthamiana Arabidopsis thaliana...
Translational efficiency change is an important mechanism for regulating protein synthesis. Experiments with paired ribosome profiling (Ribo-seq) and mRNA-sequencing (RNA-seq) allow the study of translational by simultaneously quantifying abundances total transcripts those that are being actively translated. Existing methods Ribo-seq data analysis either ignore pairing structure in experimental design or treat samples as fixed effects instead random effects. To address these issues, we...