A5079867161- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immune cells in cancer
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Semiconductor materials and devices
- Biosimilars and Bioanalytical Methods
- Tissue Engineering and Regenerative Medicine
- Advancements in Semiconductor Devices and Circuit Design
- 3D Printing in Biomedical Research
- Renal and related cancers
- Immune responses and vaccinations
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- Gold and Silver Nanoparticles Synthesis and Applications
- Histone Deacetylase Inhibitors Research
Institut Curie
2021-2024
Inserm
2021-2024
Université Paris Sciences et Lettres
2021-2024
Immunité et Cancer
2023-2024
Consejo Superior de Investigaciones Científicas
2013-2022
Centro Nacional de Biotecnología
2013-2022
National Research Council
2022
Spanish National Centre for Cardiovascular Research
2016
Exosomes are cell‐secreted nanovesicles (40–200 nm) that represent a rich source of novel biomarkers in the diagnosis and prognosis certain diseases. Despite increasingly recognized relevance these vesicles as biomarkers, their detection has been limited due part to current technical challenges rapid isolation analysis exosomes. The complexity development analytical platforms relies on heterogeneous composition exosome membrane. One most attractive tests is inmunochromatographic strips,...
Natural killer (NK) cells recognize and kill target undergoing different types of stress. NK are also capable modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing resting activated human their secreted extracellular vesicles (EVs) led to the identification a specific repertoire NK-EV-associated microRNAs post-transcriptional modifications signature. Several NK-EVs, namely miR-10b-5p, miR-92a-3p, miR-155-5p, specifically molecules...
Abstract Failure of adoptive T-cell therapies in patients with cancer is linked to limited expansion and persistence, even memory-prone 41BB-(BBz)–based chimeric antigen receptor (CAR) T cells. We show here that BBz-CAR stem/memory differentiation persistence can be enhanced through epigenetic manipulation the histone 3 lysine 9 trimethylation (H3K9me3) pathway. Inactivation H3K9 trimethyltransferase SUV39H1 enhances cell long-term protecting mice against tumor relapses rechallenges lung...
The human MICA (MHC I-related chain A) gene, encoding a ligand for the NKG2D (NKG2-D type II integral membrane protein) receptor, is highly polymorphic. A group of alleles, named 5.1 (prototype, MICA*008), produce truncated protein due to nucleotide insertion in transmembrane domain. These alleles are very frequent all populations studied and they have different biological properties, compared with full-length e.g. recruitment into exosomes, which makes them potent down-modulating receptor...
Tumour-derived exosomes can be released to serum and provide information on the features of malignancy, however, in order perform systematic studies biological samples, faster diagnostic techniques are needed, especially for detection low abundance proteins. Most human cancer cells positive at least one ligand activating immune receptor NKG2D presence plasma NKG2D-ligands associated with prognosis. Using MICA as example a tumour-derived antigen, endogenously expressed metastatic melanoma...
Therapy of metastatic melanoma advanced recently with the clinical implementation signalling pathway inhibitors, such as vemurafenib, specifically targeting mutant BRAFV600E. In general, patients experience remarkable responses under BRAF inhibitor (BRAFi) treatment but eventually progress within 6-8 months due to resistance development. Responding metastases show an increased immune cell infiltrate, including also NK cells, that, however, is no longer detectable in BRAFi-resistant lesions,...
Intravesical instillation of bacillus Calmette-Guérin (BCG) is used to treat superficial bladder cancer, either papillary tumors (after transurethral resection) or high-grade flat carcinomas (carcinoma in situ), reducing recurrence about 70% patients. Initially, BCG was proposed work through an inflammatory response, mediated by phagocytic uptake mycobacterial antigens and cytokine release. More recently, other immune effectors such as monocytes, natural killer (NK), NKT cells have been...
Immunotherapy, via intra-vesical instillations of BCG, is the therapy choice for patients with high-risk non-muscle invasive bladder cancer. The subsequent recruitment lymphocytes and myeloid cells, as well release cytokines chemokines, believed to induce a local immune response that eliminates these tumors, but detailed mechanisms action this are not understood. Here, we have studied phenotype function responding lymphocyte populations spectrum chemokines produced in an vitro model human...
Background: Intra-vesical instillation of Bacille Calmette–Guérin (BCG), an attenuated strain Mycobacterium bovis, is effective therapy for high-grade non-muscle invasive bladder cancer (NMIBC), which provokes a local immune response resulting in 70% patients free relapse after three years. Because non-responder usually have bad prognosis, the early identification treatment failure crucial. We hypothesized that, if was taking place bladder, soluble factors would be released to urine many...
High grade non-muscle-invasive bladder tumours are treated with transurethral resection followed by recurrent intravesical instillations of Bacillus Calmette Guérin (BCG). Although most cancer patients respond well to BCG, there is no clinical parameter predictive treatment response, and when fails, the prognosis very poor. Further, a high percentage NMIBC BCG suffer unwanted effects that force them stop treatment. Thus, early identification in which will fail really important. Here,...
Summary After immune interactions, membrane fragments can be transferred between cells. This fast transfer of molecules is transient and shows selectivity for certain proteins; however, the constraints underlying acquisition a protein are unknown. To characterize mechanism functional consequences this process in natural killer ( NK ) cells, we have compared different NKG 2D ligands. We show that human ligands acquired by cells with efficiencies. The main findings ligand related to activation...
Natural killer cells are cytotoxic lymphocytes important in immune responses to cancer and multiple pathogens. However, chronic activation of NK can induce a hyporesponsive state. The molecular basis the mechanisms underlying generation maintenance this condition unknown, thus an easy reproducible mechanism able hyporesponsiveness on human would be very useful gain understanding process. Human treated with ionomycin lose their ability degranulate secrete IFN-γ response variety stimuli, but...
<div>Abstract<p>Failure of adoptive T-cell therapies in patients with cancer is linked to limited expansion and persistence, even memory-prone 41BB-(BBz)–based chimeric antigen receptor (CAR) T cells. We show here that BBz-CAR stem/memory differentiation persistence can be enhanced through epigenetic manipulation the histone 3 lysine 9 trimethylation (H3K9me3) pathway. Inactivation H3K9 trimethyltransferase <i>SUV39H1</i> enhances cell long-term protecting mice...
<div>Abstract<p>Failure of adoptive T-cell therapies in patients with cancer is linked to limited expansion and persistence, even memory-prone 41BB-(BBz)–based chimeric antigen receptor (CAR) T cells. We show here that BBz-CAR stem/memory differentiation persistence can be enhanced through epigenetic manipulation the histone 3 lysine 9 trimethylation (H3K9me3) pathway. Inactivation H3K9 trimethyltransferase <i>SUV39H1</i> enhances cell long-term protecting mice...
<p>Supplementary Figures and Legends, Supplementary Table Legends Figure S1.Engineering SUV39H1-deficient human T cells. S2. cells show enhanced stem/memory less effector/exhausted phenotype. S3. SUV39H1 deletion in CAR promotes stronger rejection of liquid solid tumors. S4. Single-cell transcriptomics reveals enrichment signatures S5. Enhanced self-renewal populations. 6. memory cell signature correlates with clinical response. 7. ablation induces chromatin opening at loci all...