A5001868955- Sarcoma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Renal and related cancers
- Education, Psychology, and Social Research
- Cancer-related Molecular Pathways
- Breast Cancer Treatment Studies
- Oral and Maxillofacial Pathology
- Protein Hydrolysis and Bioactive Peptides
- Family and Disability Support Research
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
- Soft tissue tumor case studies
- Advanced Breast Cancer Therapies
- Advanced Biosensing Techniques and Applications
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Child and Adolescent Health
- Pancreatic and Hepatic Oncology Research
Masaryk University
2018-2024
University Hospital Brno
2018-2024
St. Anne's University Hospital Brno
2018-2020
Infantile myofibromatosis represents one of the most common proliferative fibrous tumors infancy and childhood. More effective treatment is needed for drug-resistant patients, targeted therapy using specific protein kinase inhibitors could be a promising strategy. To date, several studies have confirmed connection between p.R561C mutation in gene encoding platelet-derived growth factor receptor beta (PDGFR-beta) development infantile myofibromatosis. This study aimed to analyze...
Background: Tumor mutational burden (TMB) is an emerging genomic biomarker in cancer that has been associated with improved response to immune checkpoint inhibitors (ICIs) adult cancers. It was described variability TMB assessment introduced by different laboratory techniques and various settings of bioinformatic pipelines. In pediatric oncology, no study published describing this so far. Methods: our study, we performed whole exome sequencing (WES, both germline somatic) calculated 106...
The specific targeting of signal transduction by low-molecular-weight inhibitors or monoclonal antibodies represents a very promising personalized treatment strategy in pediatric oncology. In this study, we present the successful and clinically relevant use commercially available phospho-protein arrays for analyses phosphorylation profiles broad spectrum receptor tyrosine kinases their downstream signaling proteins tumor tissue samples. Although these were made research purposes on human...
In order to identify reasons for treatment failures when using targeted therapies, we have analyzed the comprehensive molecular profiles of three relapsed, poor-prognosis Burkitt lymphoma cases. All cases had resembling clinical presentation and histology all patients but their outcomes differed significantly. The samples tumor tissue were whole-exome sequencing, gene expression profiling, phosphoproteomic assays, single-cell phosphoflow cytometry. These results explain different responses...
ABSTRACT Pediatric sarcomas are bone and soft tissue tumors that often exhibit high metastatic potential refractory stem-like phenotypes, resulting in poor outcomes. Aggressive frequently harbor a disrupted p53 pathway. However, whether sarcoma stemness is associated with abrogated function might be attenuated via reactivation remains unclear. Here, we show highly tumorigenic cells dysregulated p53, making them vulnerable to drugs restore wild-type activity. Immunohistochemistry of mouse...
A lot of hope for high-risk cancers is being pinned on immunotherapy but the evidence in children lacking due to rarity and limited efficacy single-agent approaches. Here, we aim assess effectiveness multimodal therapy comprising a personalized dendritic cell (DC) vaccine with relapsed and/or solid tumors using N-of-1 approach real-world scenario. total 160 evaluable events occurred 48 patients during 4-year follow-up. Overall survival cohort was 7.03 years. Disease control after vaccination...
Introduction: The individualization of treatment is attractive, especially in children with high-risk cancer. In such a rare and very heterogeneous group diseases, large population-based clinical randomized trials are not feasible without international collaboration. We therefore propose comparative patient series analysis real-life scenario. Methods: Open cohort observational study, analysis. Seventy patients solid tumors diagnosed between 2003 2015 whom the was individualized either...
Background: Current standard chemoimmunotherapy achieves survival in almost 95% children with mature B-NHL. However, relapses still carry very poor prognosis. Methods: Molecular profiling consisted of whole exome NGS, gene expression and peripheral blood phosphoflow. Results: Both cases had identical clinical presentation histology, progressed early during therapy did not respond to intensive retrieval chemotherapy. In the first patient, a germ line variant c.935C>G PI3K-delta subunit was...