A5103189120- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Extracellular vesicles in disease
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Nanoparticle-Based Drug Delivery
- Polymer Surface Interaction Studies
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Inflammasome and immune disorders
- Microtubule and mitosis dynamics
- interferon and immune responses
- Adipose Tissue and Metabolism
- Photosynthetic Processes and Mechanisms
- Phagocytosis and Immune Regulation
- Biochemical and Molecular Research
- Alzheimer's disease research and treatments
Université du Québec à Montréal
2019-2025
Université du Québec à Trois-Rivières
2015-2025
Université du Québec
2021-2025
Nanobiotix (France)
2017
University of Ottawa
1965-2016
uOttawa Brain and Mind Research Institute
2016
University of British Columbia
2007-2008
Vancouver Coastal Health Research Institute
2007
Marie Curie
2007
École Nationale Supérieure des Mines de Paris
2007
Most cells constitutively secrete mitochondrial DNA and proteins in extracellular vesicles (EVs). While EVs are small that transfer material between cells, Mitochondria-Derived Vesicles (MDVs) carry specifically mitochondria other organelles. Mitochondrial content can enhance inflammation under pro-inflammatory conditions, though its role the absence of remains elusive. Here, we demonstrate actively prevent packaging pro-inflammatory, oxidized would act as damage-associated molecular...
BIK, a pro-apoptotic BH3-only member of the BCL-2 family, targets membrane endoplasmic reticulum (ER). It is induced in human cells response to several stress stimuli, including genotoxic (radiation, doxorubicin) and overexpression E1A or p53 but not by ER pathways resulting from protein malfolding. BIK initiates an early release Ca2+ upstream activation effector caspases. Release mobile stores baby mouse kidney doubly deficient BAX BAK, on other hand, resistant sensitive ectopic BAK....
Mitochondria are crucial for cellular metabolism and signalling. Mitochondrial activity is modulated by mitochondrial fission fusion, which required to properly balance metabolic functions, transfer material between mitochondria, remove defective mitochondria. occurs at mitochondria-endoplasmic reticulum (ER) contact sites, requires the formation of actin filaments that drive constriction recruitment protein DRP1. The role in fusion remains entirely unexplored. Here we show preventing...
Stimulation of apoptosis by p53 is accompanied induction the BH-3-only proapoptotic member BCL-2 family, BIK, and ectopic expression BIK in p53-null cells caused release cytochrome c from mitochondria activation caspases, dependent on a functional BH-3 domain. A significant fraction which contains predicted transmembrane segment at its COOH terminus, was found inserted endoplasmic reticulum (ER) membrane, with bulk protein facing cytosol. Restriction to this membrane replacing ER-selective...
Mitochondria are multifaceted organelles crucial for cellular homeostasis that contain their own genome. Mitochondrial DNA (mtDNA) replication is a spatially regulated process essential the maintenance of mitochondrial function, its defect causing diseases. mtDNA occurs at endoplasmic reticulum (ER)-mitochondria contact sites and affected by dynamics: The absence fusion associated with depletion whereas loss fission causes aggregation within abnormal structures termed mitobulbs. Here, we...
A genome wide search for new BH3‐containing Bcl‐2 family members was conducted using position weight matrices (PWM) and identified a large (480 kDa), novel BH3‐only protein, originally called LASU1 (now also known as Ureb‐1, E3 histone , ARF‐BP1, Mule). We demonstrated that is an ligase ubiquitinated Mcl‐1 in vitro required its proteasome‐dependent degradation HeLa cells. Of note, the BH3 domain of interacted with but not or Bcl‐Xl. competing BH3‐ligand derived from Bim prevented interaction...
Parkinson’s disease (PD) is a progressive neurodegenerative disorder, primarily affecting dopaminergic neurons in the substantia nigra. There currently no cure for PD and present medications aim to alleviate clinical symptoms, thus prevention remains ideal strategy reduce prevalence of this disease. The goal study was investigate whether oleuropein (OLE), major phenolic compound olive derivatives, may prevent neuronal degeneration cellular model PD, differentiated PC12 cells exposed potent...
Abstract Oocyte maturation is a key process during which the female germ cell undergoes resumption of meiosis and completes its preparation for embryonic development including cytoplasmic epigenetic maturation. The cumulus cells directly surrounding oocyte are involved in this by transferring essential metabolites, such as pyruvate, to oocyte. This controlled cyclic adenosine monophosphate (cAMP)-dependent mechanisms recruited downstream follicle-stimulating hormone (FSH) signaling cells. As...
Persistence of drug-resistant breast cancer stem cells (brCSCs) after a chemotherapeutic regime correlates with disease recurrence and elevated mortality. Therefore, deciphering mechanisms that dictate their phenotype is imperative for designing targeted more effective therapeutic strategies. The transcription factor SOX2 has been recognized as protagonist in brCSC maintenance, previous studies have confirmed inhibition purportedly eliminated these brCSCs. However, pharmacological targeting...
The BCL-2 homologue MCL-1 plays an important role in the regulation of cell fate by blocking apoptosis as well regulating cycle. has unusual N-terminal extension, which contains a PEST domain and several phosphorylation sites that have been suggested to regulate its turnover. Here we report first 79 amino acids subcellular localization. Deletion this impairs both mitochondrial localization anti-apoptotic activity. Conversely, expression N terminus promotes association with mitochondria...
Mitochondria exist as a highly interconnected network that is exquisitely sensitive to variations in nutrient availability, well large array of cellular stresses. Changes length and connectivity this network, alterations the mitochondrial inner membrane (cristae), regulate cell fate by controlling metabolism, proliferation, differentiation, death. Given key roles dynamics, process which mitochondria constantly fuse fragment, measure provides crucial information on health activity various...
Peripheral neuropathies are often caused by disruption of genes responsible for myelination or axonal transport. In particular, impairment in mitochondrial fission and fusion known causes peripheral neuropathies. However, the causal mechanisms neuropathy gene mutations not always known. While loss function MYH14 typically cause non-syndromic hearing loss, recently described R941L mutation MYH14, encoding non-muscle myosin protein isoform NMIIC, leads to a complex clinical presentation with...
Here we present three different types of mechanically stable nanometer-sized hollow capsules. The common point the currently developed systems in our laboratory is that they are liposome based. Biomolecules can be used to functionalize lipid vesicles create a new type intelligent material. For example, insertion membrane channels into capsule wall modify permeability. Covalent binding antibodies allows targeting specific sites. Liposomes loaded with enzymes may provide an optimal environment...
The BCL-2 family of proteins plays a major role in the control apoptosis as primary regulator mitochondrial permeability. pro-apoptotic homologues BAX and BAK are activated following induction induce cytochrome c release from mitochondria. A second class homologues, BH3-only proteins, is required for activation BAK. activity both BAX/BAK opposed by anti-apoptotic such MCL-1. Here we show that MCL-1 inhibits function downstream its initial translocation to Although interacted with inhibited...