A5051793026- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Actinomycetales infections and treatment
- Metal complexes synthesis and properties
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- Microbial Metabolism and Applications
- Biochemical and Molecular Research
- Marine Sponges and Natural Products
- Mycorrhizal Fungi and Plant Interactions
- Radioactive element chemistry and processing
- Bacterial biofilms and quorum sensing
- Signaling Pathways in Disease
- Chemical synthesis and alkaloids
- Fungal Infections and Studies
- Mine drainage and remediation techniques
- Organometallic Complex Synthesis and Catalysis
- Infectious Diseases and Mycology
Universidad de Oviedo
2015-2024
Instituto de Investigación Sanitaria del Principado de Asturias
2021-2022
University of Southern Denmark
2009-2012
ABSTRACT Despite the fact that most industrial processes for secondary metabolite production are performed with submerged cultures, a reliable developmental model Streptomyces under these culture conditions is lacking. With exception of few species which sporulate conditions, it assumed no morphological differentiation take place. In this work, we describe new features coelicolor A3(2) grown in liquid cultures and integrate them into analogous to one previously described surface cultures....
Macroscopic growths at geographically separated acid mine drainages (AMDs) exhibit distinct populations. Yet, local heterogeneities are poorly understood. To gain novel mechanistic insights into this, we used OMICs tools to profile microbial populations coexisting in a single pyrite gallery AMD (pH ∼2) three compartments: two from stratified streamer (uppermost oxic and lowermost anoxic sediment-attached strata) one submerged non-stratified mat biofilm. The communities colonising those the...
Nutraceutical compounds as plant flavonoids play an important role in prevention and modulation of diverse heath conditions, they exert interesting antifungal, antibacterial, antioxidant antitumor effects. They also possess anti-inflammatory activities arthritis, cardiovascular disease or neurological diseases, well modulatory effects on the CYP450 activity drugs. Most are bioactive molecules origin, but their industrial production is sometimes hindered due to reasons low concentration...
Streptomyces is a diverse group of gram-positive microorganisms characterised by complex developmental cycle. Streptomycetes produce number antibiotics and other bioactive compounds used in the clinic. Most screening campaigns looking for new molecules from actinomycetes have been performed empirically, e.g., without considering whether bacteria are growing under best conditions secondary metabolite production. These were extremely productive discovered during so-called “golden age...
Streptomycetes are bacterial species that undergo a complex developmental cycle includes programmed cell death (PCD) events and sporulation. They widely used in biotechnology because they produce most clinically relevant secondary metabolites. Although Streptomyces coelicolor is one of the bacteria encoding largest number eukaryotic type kinases, biological role protein phosphorylation this bacterium has not been extensively studied before. In issue, variations phosphoproteome S. were...
Development-associated cell-death processes were investigated in detail during the growth and differentiation of Streptomyces antibioticus ATCC 11891 on confluent surface cultures, by using fluorescent viability probes, membrane activity fluorescence indicators, electron microscopy analysis. A previously unsuspected complexity was revealed, namely presence a very young compartmentalized mycelium that dies following an orderly pattern, leaving alternating live dead segments same hypha. This...
Streptomycetes are very important industrial bacteria, which produce two thirds of all clinically relevant secondary metabolites. They have a complex developmental-cycle in an early compartmentalized mycelium (MI) differentiates to multinucleated (MII) that grows inside the culture medium (substrate mycelium) until it starts growth into air (aerial and ends up forming spores. Streptomyces developmental studies focused mainly on later stages MII differentiation sporulation), with regulation...
Streptomyces species produce many clinically relevant secondary metabolites and exhibit a complex development that includes hyphal differentiation sporulation in solid cultures. Industrial fermentations are usually performed liquid cultures, conditions which strains generally do not sporulate, it was traditionally assumed no took place. The aim of this work to compare the transcriptomes S. coelicolor growing deepening knowledge differentiation. Microarrays demonstrated gene expression...
Programmed cell death (PCD) is an active cellular suicide that occurs in eukaryotes and bacteria response to both abiotic biotic stresses. In contrast eukaryotic apoptosis, little known about the molecular machinery regulates bacterial PCD. a previous work, we described existence of PCD phenomena Streptomyces (Manteca et al., Res. Microbiol. 2006, 157, 143–152). present study, performed proteomic analysis coelicolor, for which developed system obtain dead live cell-enriched samples. this...
Streptomyces species produce many clinically important secondary metabolites, including antibiotics and antitumorals. They have a complex developmental cycle, programmed cell death phenomena, that makes this bacterium multicellular prokaryotic model. There are two differentiated mycelial stages: an early compartmentalized vegetative mycelium (first mycelium) multinucleated reproductive (second arising after processes. In the present study, we made detailed proteomics analysis of distinct...
Streptomyces species produce many clinically important secondary metabolites and present a complex developmental cycle that includes programmed cell death (PCD) phenomena sporulation. Industrial fermentations are usually performed in liquid cultures, conditions which strains generally do not sporulate, it was traditionally assumed no differentiation took place. Recently, the existence of an early compartmentalized mycelium (MI) later multinucleated (MII) were described solid cultures. The...
Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves formation long multinucleated hyphae during vegetative growth, with occasional cross-walls separating compartments. Reproduction occurs by specialized aerial hyphae, which differentiate into chains uninucleoid spores. While tubulin-like FtsZ protein is required all peptidoglycan-based septa in Streptomyces, canonical divisome-dependent cell division only sporulation. Here...
Abstract This work contributes to the understanding of cell wall modifications during sporulation and germination in Streptomyces by assessing biological function biochemical properties SCO4439, a D-alanyl-D-alanine carboxypeptidase (DD-CPase) constitutively expressed development. SCO4439 harbors DD-CPase domain putative transcriptional regulator domain, separated transmembrane region. The recombinant protein shows that DD -CPase activity is inhibited penicillin G. spores SCO4439::Tn5062...
Morphogenesis in streptomycetes is characterized by the formation of aerial hyphae that emerge from substrate mycelium. Despite many years study, a detailed picture events occur during transition to mycelium has yet be defined. In this paper, it was shown specific cell death event takes place early growth Streptomyces coelicolor and lividans. Subsequently, second starts develop remaining viable segments these form islands, which progressively cover plate surface. Interestingly, genes coding...
ABSTRACT Streptomyces development was analyzed under conditions resembling those in soil. The mycelial growth rate much lower than that standard laboratory cultures, and the life span of previously named first compartmentalized mycelium remarkably increased.
Streptomycetes are multicellular bacteria with complex developmental cycles. They of biotechnological importance as they produce most bioactive compounds used in biomedicine,
Abstract Streptomyces are aerobic Gram-positive bacteria characterized by a complex life cycle that includes hyphae differentiation and spore formation. Morphological is triggered stressful conditions takes place in pro-oxidant environment, which sets the basis for an involvement of oxidative stress response this cellular process. Characterization phenotypic traits natalensis Δ katA1 (mono-functional catalase) catR (Fur-like repressor expression) strains solid medium revealed both mutants...
ArgR is a well-characterized transcriptional repressor controlling the expression of arginine and pyrimidine biosynthetic genes in bacteria. In this work, biological role Streptomyces coelicolor was analysed by comparing transcriptomes S. ∆argR its parental strain, M145, at five different times over 66-hour period. The effect more widespread than that orthologous protein Escherichia coli, affecting 1544 along microarray time series. This regulator repressed genes, but it also modulated not...