A5001885091- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Chromosomal and Genetic Variations
- Estrogen and related hormone effects
- Spaceflight effects on biology
- Cancer, Hypoxia, and Metabolism
- Genomics and Phylogenetic Studies
- Hormonal Regulation and Hypertension
- Metabolism, Diabetes, and Cancer
- Genetics and Neurodevelopmental Disorders
- NF-κB Signaling Pathways
- Nuclear Receptors and Signaling
- Genetic diversity and population structure
- Circadian rhythm and melatonin
- Botanical Research and Applications
- Plant Molecular Biology Research
- Growth Hormone and Insulin-like Growth Factors
- PARP inhibition in cancer therapy
- Pluripotent Stem Cells Research
Karolinska Institutet
2015-2024
Integrated Cardio Metabolic Centre
2018-2020
Institut de Biologia Molecular de Barcelona
2011-2017
Karolinska University Hospital
2016
Consejo Superior de Investigaciones Científicas
2011-2015
Universitat Pompeu Fabra
2008-2014
Institució Catalana de Recerca i Estudis Avançats
2014
Centro de Investigacion Principe Felipe
2014
Barcelona Biomedical Research Park
2008-2009
Abstract Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different have specific roles regulation nuclear processes or differentially distributed throughout genome. Using variant-specific antibodies to and hemagglutinin (HA)-tagged recombinant expressed breast cancer cells, we investigated distribution six promoters genome-wide. depleted at...
Aging strongly influences human morbidity and mortality. Thus, aging-preventive compounds could greatly improve our health lifespan. Here we screened for such compounds, known as geroprotectors, employing the power of transcriptomics to predict biological age. Using age-stratified tissue transcriptomes machine learning, generated age classifiers applied these transcriptomic changes induced by 1,309 different in cells, ranking their ability induce a "youthful" transcriptional state. Testing...
There are seven linker histone variants in human somatic cells (H1.0 to H1.5 and H1X), their prevalence varies as a function of cell type differentiation stage, suggesting that the different may have distinct roles. We revisited this notion by using new methodologies study pluripotency differentiation, including vitro embryonic stem (ES) teratocarcinoma reprogramming keratinocytes induced pluripotent cells. Our results show (PCs) decreased levels H1.0 increased H1.1, H1.3, compared with...
Histone H1 has seven variants in human somatic cells and contributes to chromatin compaction transcriptional regulation. Knock-down (KD) of each variant breast cancer results altered gene expression proliferation differently a specific manner with H1.2 H1.4 KDs being most deleterious. Here we show combined depletion strong deleterious effect resulting interferon (IFN) response, as evidenced by an up-regulation many IFN-stimulated genes (ISGs) not seen individual nor other combinations KDs....
PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) catalyses the synthesis and degradation of Fru-2,6-P2 (fructose-2,6-bisphosphate), a key modulator glycolysis gluconeogenesis. The PFKFB3 gene is extensively involved in cell proliferation owing to its role carbohydrate metabolism. In present study we analyse mechanism regulation by progestins breast cancer cells. We report that exposure T47D cells synthetic (ORG2058 or norgestrel) leads rapid increase concentration. Our Western...
Steroid hormone receptors regulate gene expression, interacting with target DNA sequences but also activating cytoplasmic signaling pathways. Using the human 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) as a model, we have investigated contributions of both effects on progesterone-responsive promoter in breast cancer cells. Chromatin immunoprecipitation has identified two different mechanisms hormone-induced progesterone receptor (PR) recruitment to 11β-HSD2 promoter: (i) direct PR...
Steroid hormone receptors act directly in the nucleus on chromatin organization and transcriptional activity of several promoters. Furthermore, they have an indirect effect cytoplasmic signal transduction pathways, including MAPK, impacting ultimately gene expression. We are interested distinguishing between two modes action progesterone receptor (PR) control expression cell proliferation. For this, we stably expressed, PR-negative breast cancer cells, tagged forms PR isoform B mutated at...
In mammals, the linker histone H1, involved in DNA packaging into chromatin, is represented by a family of variants. H1 tails undergo post‐translational modifications (PTMs) that can be detected mass spectrometry. We developed antibodies to analyze several these as yet unexplored PTMs including combination H1.4 K26 acetylation or trimethylation and S27 phosphorylation. H1.2‐T165 phosphorylation was at S G2/M phases cell cycle dispensable for chromatin binding proliferation; while H1.4‐K26...
Abstract Histones are the major proteinaceous component of chromatin in eukaryotic cells and an important part epigenome, affecting most DNA‐related events, including transcription, DNA replication, chromosome segregation. The properties histones greatly influenced by their post‐translational modifications (PTMs), over 200 which known today. Given this large number, researchers need sophisticated methods to study histone PTMs comprehensively. In particular, mass spectrometry (MS)−based...
The ever decreasing cost of Next-Generation Sequencing coupled with the emergence efficient and reproducible analysis pipelines has rendered genomic methods more accessible. However, downstream analyses are basic or missing in most workflows, creating a significant barrier for non-bioinformaticians. To help close this gap, we developed Cactus, an end-to-end pipeline analyzing ATAC-Seq mRNA-Seq data, either separately jointly. Its Nextflow-, container-, virtual environment-based architecture...
Summary Aging is a major risk factor for human morbidity and mortality. Thus, the identification of compounds that defer aging, also known as ‘geroprotectors’, could greatly improve our health promote longer life. Here we screened geroprotectors, employing power transcriptomics to predict biological age. We used age-stratified tissue transcriptomes generate machine-learning-based classifiers capable distinguishing from young versus old individuals. Then applied these induced by 1300...
The ever decreasing cost of Next-Generation Sequencing coupled with the emergence efficient and reproducible analysis pipelines has rendered genomic methods more accessible. However, downstream analyses are basic or missing in most workflows, creating a significant barrier for non-bioinformaticians. To help close this gap, we developed Cactus, an end-to-end pipeline analyzing ATAC-Seq mRNA-Seq data, either separately jointly. Its Nextflow-, container-, virtual environment-based architecture...