A5068554777- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA modifications and cancer
- Metal-Catalyzed Oxygenation Mechanisms
- HIV Research and Treatment
- Enzyme Structure and Function
- Porphyrin Metabolism and Disorders
- HIV/AIDS drug development and treatment
- Estrogen and related hormone effects
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Bioinformatics and Genomic Networks
- Metal complexes synthesis and properties
- Pluripotent Stem Cells Research
- Digestive system and related health
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Redox biology and oxidative stress
- Nutrition, Genetics, and Disease
Institut de Biologia Molecular de Barcelona
2016-2025
Consejo Superior de Investigaciones Científicas
1983-2024
Universitat Pompeu Fabra
2006-2014
Institució Catalana de Recerca i Estudis Avançats
2014
Centro de Investigacion Principe Felipe
2014
Barcelona Biomedical Research Park
2008-2009
Universidad de Sevilla
2009
Centre for Genomic Regulation
2008
Karolinska Institutet
1996-2004
Universitat Autònoma de Barcelona
1994-2003
At least six histone H1 variants exist in somatic mammalian cells that bind to the linker DNA and stabilize nucleosome particle contributing higher order chromatin compaction. In addition, seems be actively involved regulation of gene expression. However, it is not well known whether different have distinct roles or if they regulate specific promoters. We explored this by inducible shRNA-mediated knock-down each a human breast cancer cell line. Rapid inhibition variant was compensated for...
Abstract Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different have specific roles regulation nuclear processes or differentially distributed throughout genome. Using variant-specific antibodies to and hemagglutinin (HA)-tagged recombinant expressed breast cancer cells, we investigated distribution six promoters genome-wide. depleted at...
Ribonucleotides are converted to deoxyribonucleotides by ribonucleotide reductases. Either thioredoxin or glutaredoxin is a required electron donor for class I and II enzymes. Glutaredoxins reduced glutathione, thioredoxins reductase. Recently, glutaredoxin-like protein, NrdH, was isolated as the functional NrdEF reductase, Ib enzyme, from Lactococcus lactis. The absence of glutathione in this bacterium raised question identity intracellular reductant NrdH. Homologues NrdH present genomes...
There are seven linker histone variants in human somatic cells (H1.0 to H1.5 and H1X), their prevalence varies as a function of cell type differentiation stage, suggesting that the different may have distinct roles. We revisited this notion by using new methodologies study pluripotency differentiation, including vitro embryonic stem (ES) teratocarcinoma reprogramming keratinocytes induced pluripotent cells. Our results show (PCs) decreased levels H1.0 increased H1.1, H1.3, compared with...
The establishment of a stable reservoir latently infected cells allows HIV to persist in the host. Usually, infection T results integration viral genome, with preference for regions human genome containing active genes, expression, and production new viruses. However, rare cases become infected, this is presumed be due combination two factors: integrated viruses are not efficiently transcribed revert resting memory state. latency has been associated provirus constitutive heterochromatin,...
Histone H1 has seven variants in human somatic cells and contributes to chromatin compaction transcriptional regulation. Knock-down (KD) of each variant breast cancer results altered gene expression proliferation differently a specific manner with H1.2 H1.4 KDs being most deleterious. Here we show combined depletion strong deleterious effect resulting interferon (IFN) response, as evidenced by an up-regulation many IFN-stimulated genes (ISGs) not seen individual nor other combinations KDs....
The nrdA and nrdB genes of Escherichia coli Salmonella typhimurium encode the R1 R2 proteins that together form an active class I ribonucleotide reductase. Both organisms contain two additional chromosomal genes, nrdE nrdF, whose corresponding protein sequences show some homology to products nrdB. When present on a plasmid, nrdF complement mutations in or We have now obtained nearly homogeneous encoded by S. (R1E R2F). They correspond proteins. Each is homodimer. Together they catalyze...
The first class Ib ribonucleotide reductase R2 structure, from Salmonella typhimurium, has been determined at 2.0 Å resolution. overall structure is similar to the Escherichia coli Ia enzyme despite only 23% sequence identity. most spectacular difference absence of pleated sheet and adjacent parts present in E. structure; heart-shaped loses its tip. From comparisons, it appears that this feature shared with all other enzymes and, respect, more like mammalian enzymes. Both oxidized reduced...
Escherichia coli contains the genetic information for three separate ribonucleotide reductases. Two of them (class I enzymes), coded by nrdAB and nrdEF genes, respectively, contain a tyrosyl radical, whose generation requires oxygen. The NrdAB enzyme is physiologically active. function gene not known. third III), nrdDG, operates during anaerobiosis. DNA Lactococcus lactis sequences homologous to nrdDG genes. Surprisingly, an nrdD mutant L. grew well under standard anaerobic growth...
Upon HIV-1 infection, a reservoir of latently infected resting T cells prevents the eradication virus from patients. To achieve complete depletion, existing virus-suppressing antiretroviral therapy must be combined with drugs that reactivate dormant viruses. We previously described novel chemical scaffold compound, MMQO (8-methoxy-6-methylquinolin-4-ol), is able to viral transcription in several models HIV latency, including J-Lat cells, through an unknown mechanism. potentiates activity...
Abstract Up to seven members of the histone H1 family may contribute chromatin compaction and its regulation in human somatic cells. In breast cancer cells, knock-down multiple variants deregulates many genes, promotes appearance genome-wide accessibility sites triggers an interferon response via activation heterochromatic repeats. However, how these changes expression profile relate re-distribution as well genome conformational have not been yet studied. Here, we combined ChIP-seq five...
Previously we discovered that among 15 DNA-binding plant secondary metabolites (PSMs) possessing anticancer activity, 11 compounds cause depletion of the chromatin-bound linker histones H1.2 and/or H1.4. Chromatin remodeling or multiH1 knocking-down is known to promote upregulation repetitive elements, ultimately triggering an interferon (IFN) response. Herein, using HeLa cells and applying fluorescent reporter assay with flow cytometry, immunofluorescence staining quantitative RT-PCR,...
Histone H1 is involved in the regulation of chromatin structure and gene expression. Up to seven subtypes or variants are expressed human somatic cells. The complement, defined as subtype composition proportions a given cell, highly variable depending on cell type, cycle, developmental stage, several diseases such cancer. It result combined action different regulatory processes. Epitranscriptome modifications have emerged new mechanism able control all aspects mRNA metabolism. In this work,...
Salmonella typhimurium and Escherichia coli cells have two different class I ribonucleotide reductases encoded by the nrdEF nrdAB operons. Despite presence of one additional reductase, ‐encoded enzyme is essential to aerobic growth cell because ‐defective mutants both species are not viable in oxygen. Several factors controlling gene transcription been analysed intensively. Nothing known about expression genes. To study this subject, after cloning E. genes sequencing their 5′ ends, promoter...
PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) catalyses the synthesis and degradation of Fru-2,6-P2 (fructose-2,6-bisphosphate), a key modulator glycolysis gluconeogenesis. The PFKFB3 gene is extensively involved in cell proliferation owing to its role carbohydrate metabolism. In present study we analyse mechanism regulation by progestins breast cancer cells. We report that exposure T47D cells synthetic (ORG2058 or norgestrel) leads rapid increase concentration. Our Western...