A5034451967- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Metal-Catalyzed Oxygenation Mechanisms
- Bacterial Genetics and Biotechnology
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Porphyrin Metabolism and Disorders
- Metalloenzymes and iron-sulfur proteins
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Tuberculosis Research and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Biosensors and Analytical Detection
- Cystic Fibrosis Research Advances
- Bladder and Urothelial Cancer Treatments
- Photosynthetic Processes and Mechanisms
- Oral microbiology and periodontitis research
- Invertebrate Immune Response Mechanisms
Institute for Bioengineering of Catalonia
2016-2025
Universitat de Barcelona
1996-2025
Instituto de Biomedicina de Valencia
2022
Barcelona Institute for Science and Technology
2018-2019
Shanghai Institute for Science of Science
2018-2019
Institute of Science and Technology
2017-2019
Stockholm University
2005-2011
Universitat Autònoma de Barcelona
1996-2008
Karolinska Institutet
1997-2001
Ludwig Cancer Research
1997
Six morpholine-(iso)thiosemicarbazone hybrids HL1-HL6 and their Cu(II) complexes with good-to-moderate solubility stability in water were synthesized characterized. [Cu(L1-6)Cl] (1-6) formed weak dimeric associates the solid state, which did not remain intact solution as evidenced by ESI-MS. The lead proligands displayed higher antiproliferative activity cancer cells than triapine. In addition, 2-5 found to specifically inhibit growth of Gram-positive bacteria Staphylococcus aureus MIC50...
Abstract Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as biofilms, are associated with several infectious diseases. One main components these biofilms is alginate, homo- hetero-polysaccharide that consists β-D-mannuronate (M) α-L-guluronate (G) units. Alginate lyases degrade this sugar have been proposed biotherapeutic agents to dissolve P. biofilms. However, there contradictory reports in literature regarding...
The coexistence between species that occurs in some infections remains hard to achieve vitro since bacterial fitness differences eventually lead a single organism dominating the mixed culture. Pseudomonas aeruginosa and Staphylococcus aureus are major pathogens found growing together biofilms disease-affected lungs or wounds. Herein, we tested analyzed different culture media, additives environmental conditions support P. S. vitro. We have unraveled potential of DMEM growth these two...
The low efficacy of current conventional treatments for bacterial infections increases mortality rates worldwide. To alleviate this global health problem, we propose drug-free enzyme-based nanomotors the treatment urinary-tract infections. We develop consisting mesoporous silica nanoparticles (MSNPs) that were functionalized with either urease (U-MSNPs), lysozyme (L-MSNPs), or and (M-MSNPs), use them against nonpathogenic planktonic Escherichia coli. U-MSNPs exhibited highest bactericidal...
Abstract Background Ribonucleotide reductases (RNRs) catalyse the only known de novo pathway for deoxyribonucleotide synthesis, and are therefore essential to DNA-based life. While ribonucleotide reduction has a single evolutionary origin, significant differences between RNRs nevertheless exist, notably in cofactor requirements, subunit composition allosteric regulation. These result distinct operational constraints (anaerobicity, iron/oxygen dependence cobalamin dependence), form basis...
The cellular prion protein, encoded by the gene Prnp, has been reported to be a receptor of β-amyloid. Their interaction is mandatory for neurotoxic effects β-amyloid oligomers. In this study, we aimed explore whether protein participates in spreading α-synuclein. Results demonstrate that Prnp expression not α-synuclein spreading. However, although pathological can take place absence expanded faster PrPC-overexpressing mice. addition, binds strongly on PrPC-expressing cells, suggesting role...
Oleanolic acid (OA) and maslinic (MA) are pentacyclic triterpenic compounds that abound in industrial olive oil waste. These have renowned antimicrobial properties lack cytotoxicity eukaryotic cells as well resistance mechanisms bacteria. Despite these advantages, their activity has only been tested vitro, derivatives improving this not reported. In work, a set of 14 OA MA C-28 amide synthesized. Two derivatives, MA-HDA OA-HDA, increase the vitro parent while reducing toxicity most...
Escherichia coli possesses class Ia, Ib, and III ribonucleotide reductases (RNR). Under standard laboratory conditions, the aerobic Ia nrdAB RNR genes are well expressed, whereas Ib nrdEF poorly expressed. The is normally expressed under microaerophilic anaerobic conditions. In this paper, we show that E. YbaD protein differentially regulates expression of three sets genes. a homolog Streptomyces NrdR protein. It not essential for growth has been renamed NrdR. Previously, was shown to...
Abstract Background Ribonucleotide reduction is the only de novo pathway for synthesis of deoxyribonucleotides, building blocks DNA. The reaction catalysed by ribonucleotide reductases (RNRs), an ancient enzyme family comprised three classes. Each class has distinct operational constraints, and are broadly distributed across organisms from all domains, though few I RNRs have been identified in archaeal genomes, classes II III likewise appear rare eukaryotes. In this study, we examine whether...
Abstract Dielectrophoresis (DEP) represents a powerful approach to manipulate and study living cells. Hitherto, several approaches have used 2‐D DEP chips. With the aim increase sample volume, in this we 3‐D carbon‐electrode chip trap release bacterial A continuous flow was plug an Escherichia coli cell suspension first, retain cells by positive DEP, thereafter recover them washing with peptone water solution. This allows one not only analyze behavior of within chip, but also further...
Currently, three major circumstances threaten the management of bacterial infections: increasing antimicrobial resistance, expansion chronic biofilm-associated infections, and lack an appropriate approach to treat them. To date, development accelerated drug susceptibility testing biofilms new antibiofouling systems has not been achieved despite availability different methodologies. There is a need for easy-to-use methods antibiotic bacteria that form screening possible antibiofilm...
Abstract Microbial biofilms are complex three‐dimensional structures where sessile microbes embedded in a polymeric extracellular matrix. Their resistance toward the host immune system as well to diverse range of antimicrobial treatments poses serious health and development threat, being top 10 global public threats declared by World Health Organization. In an effort combat biofilm‐related microbial infections, several strategies have been developed independently eliminate or complement...
Historical processes in community assembly, such as species arrival order, influence interactions, causing priority effects. Candida albicans and Pseudomonas aeruginosa often co-occur biofilm-based infections of the skin, lungs, medical devices. Their predominantly antagonistic relationship involves complex physical chemical interactions. However, presence implications effects among these microorganisms remain largely unexplored. Here, we investigate impact effect dual-species biofilms using...
Ribonucleotide reductases (RNRs) are the essential enzymes responsible for synthesizing dNTPs, building blocks of DNA. In bacteria, entire RNR network is controlled by master regulator NrdR. As a an pathway with no eukaryotic equivalent, NrdR promising antimicrobial target. Recent studies have outlined mechanism action NrdR, in which ATP and dATP induce changes protein quaternary structure, regulating repression. However, due to lack functional linking known structures their biological...
The Streptococcus pyogenes genome harbors two clusters of class Ib ribonucleotide reductase genes, nrdHEF and nrdF*I*E*, a second stand-alone nrdI gene, designated nrdI2. We show that both are expressed simultaneously as independent operons. NrdEF enzyme is functionally active in vitro, while the NrdE*F* not. NrdF* protein lacks three six highly conserved iron-liganding side chains cannot form dinuclear iron site or tyrosyl radical. In vivo, on other hand, operons functional heterologous...
Abstract The extracellular matrix protects biofilm cells by reducing diffusion of antimicrobials. Tobramycin is an antibiotic used extensively to treat P. aeruginosa biofilms, but it sequestered in the periphery negative charge and loses its efficacy significantly. Dispersal with enzymes such as DNase I another promising therapy that enhances into biofilm. Here, we combine neutralization tobramycin provided dextran-based single-chain polymer nanoparticles (SCPNs) together break matrix. Our...
Escherichia coli is one of the most common members intestinal microbiota. Many its strains are associated with various inflammatory infections, including urinary or gut especially when displaying antibiotic resistance in patients suppressed immune systems. According to recent reports, biofilm-forming potential E. a crucial factor for increased against antibiotics. To overcome limitations using antibiotics resistant strains, world turning once more towards bacteriophage therapy, which...