The coexistence between species that occurs in some infections remains hard to achieve vitro since bacterial fitness differences eventually lead a single organism dominating the mixed culture. Pseudomonas aeruginosa and Staphylococcus aureus are major pathogens found growing together biofilms disease-affected lungs or wounds. Herein, we tested analyzed different culture media, additives environmental conditions support P. S. vitro. We have unraveled potential of DMEM growth these two...

The reductive amination reaction of imines catalyzed by Knölker-type iron complexes under hydrogen at high pressure is very interesting in synthetic terms. This type an important catalytic challenge, since harsh conditions are necessary and do not occur easily. In a previous work ( Organometallics 2022, 41, 1204−1215), we carried out computational study the mechanism showing that electron-withdrawing groups (EWGs) attached to cyclopentadienone favor imines. synthesis with cyclopentadienones...

Pseudomonas aeruginosa is the principal cause of bacterial keratitis worldwide and overstimulation innate immune system by this organism believed to contribute significantly sight loss. In current study, we have used primary human corneal fibroblast (hCF) cells as an ex vivo model infection examine role P. flagellum type three secretion (TTSS) in inducing inflammasome-associated molecules that trigger IL-1β IL-18 production during early stages infection. Our results show stimulated...

Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead infection persistence recurrence, thus worsening outcomes. Lung infections caused Pseudomonas aeruginosa, especially in cystic fibrosis (CF) are often aggravated of pathogen. Proliferation infectious species relies on a continuous deoxyribonucleotide (dNTP) supply, for which ribonucleotide reductase enzyme (RNR)...

The intestinal mucus lines the luminal surface of epithelium. This is a dynamic semipermeable barrier and one first-line defense mechanisms against outside environment, protecting body chemical, mechanical, or biological external insults. At same time, accommodates resident microbiota, providing nutrients attachment sites, therefore playing an essential role in host-pathogen interactions gut homeostasis. Underneath this layer, epithelium organized into finger-like protrusions called villi...

A critical step in the life cycle of all organisms is duplication genetic material during cell division. Ribonucleotide reductases (RNRs) are essential enzymes for this because they control de novo production deoxyribonucleotides required DNA synthesis and repair. Enterobacteriaceae have three functional classes RNRs (Ia, Ib, III), which transcribed from separate operons encoded by genes nrdAB, nrdHIEF, nrdDG, respectively. Here, we investigated role virulence adherent-invasive Escherichia...

Ribonucleotide reductase (RNR) is an essential enzyme for all living organisms since the responsible last step in synthesis of four deoxyribonucleotides (dNTPs) necessary DNA replication and repair. In this work, we have investigated expression three-RNR classes (Ia, Ib III) during Escherichia coli biofilm formation. We show temporal spatial importance class III RNRs process two different E. wild-type strains, commensal MG1655 enteropathogenic virulent E2348/69, prototype (EPEC). established...

Abstract Designing useful functionalities in clinically validated, old antibiotics holds promise to provide the most economical solution for global lack of effective antibiotics, as undoubtedly a serious health threat. Here we show that using surface chemistry cyclodextrin (βCD) cycle and arginine (arg) linker, provides more stable ternary antibiotic complex (βCD-arg-cpx). In contrast classical less inclusion complexes, which only modify solubility, here-presented is controls drug release....

Ribonucleotide reductases (RNRs) are essential enzymes for DNA synthesis because they responsible the production of four deoxyribonucleotides (dNTPs) from their corresponding ribonucleotides. Escherichia coli contains two classes aerobic RNRs, encoded by nrdAB (class Ia) and nrdHIEF Ib) operons, a third RNR class, which is functional under anaerobic conditions nrdDG III) operon. Because cellular imbalances in amounts dNTPs cause an increase rate mutagenesis, activity expression RNRs must be...

Streptococcus pneumoniae (Spn) is a major causative organism of empyema, an inflammatory condition occurring in the pleural sac. In this study, we used human and Spn cDNA microarrays to characterize transcriptional responses during initial contact between mesothelial cell line (PMC) vitro. Using stringent filtering criteria, 42 23 genes were up-and down-regulated respectively. particular, encoding factors potentially involved metabolic processes adherence eukaryotic cells up-regulated e.g....

Proteins of the Hha/YmoA family co‐regulate with H‐NS expression virulence factors in Enterobacteriaceae . Vibrio cholerae lacks Hha‐like proteins and its (vcH‐NS) is unable to bind Hha, spite conservation a key residue for Hha binding by Escherichia coli (ecH‐NS). Exchange residues position 9 between vcH‐NS ecH‐NS strongly reduces introduces it vcH‐NS. These mutations affect repression hemolysin operon E. electrophoretic mobility complexes formed DNA fragment containing regulatory region.

Abstract Bacterial keratitis (BK) is an ocular disorder associated with poor visual prognosis. Quantification of the inflammatory response may provide insight into pathogenesis BK and guide treatment options. In this exploratory study, we evaluated 45 patients 20 healthy controls by optical coherence tomography pro-inflammatory tear cytokine analysis. The aim was to quantify differential morphological between Gram-negative Gram-positive determine diagnostic value corneal thickness (CT)...

Contact-based antimicrobials, as antibiotic-free technologies that use non-specific interactions with bacterial cells to exert antimicrobial activity, are a prospective solution in fighting the global issue of resistance. A very simplified approach their design considers direct bonding cationic guanidine-containing amino acids surface nano-gold carriers. The structure enables activity due high density charges. This opens set novel questions important for effective engineering, particularly...

Pseudomonas aeruginosa is a major cause of bacterial keratitis (BK) worldwide. Inappropriate or non-optimal antibiotic chemotherapy can lead to corneal perforation and rapid sight loss. In this study, we tested the hypothesis that P. strain PAO1 invades primary human fibroblasts (hCFs) in vitro persists intracellularly, despite with antibiotics used commonly treat BK. rank order, ciprofloxacin, levofloxacin polymyxin B showed highest activity against planktonic growth (100% inhibitory...

To examine over time, the electron microscopic changes associated with Pseudomonas aeruginosa (PA) and human corneal tissue interactions in context of microbial keratitis. Corneal stromal fibroblast monolayer whole models were made from eye bank eyes residual after transplantation. In model (WTM), donor buttons infected PAO1 by scoring intrastromal injection. Tissue was examined 3, 9 24 h post challenge transmission microscopy (TEM) scanning (SEM). cell culture (CCM), fibroblasts (CF) vitro...

An amendment to this paper has been published and can be accessed via a link at the top of paper.