A5052996829- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Genetic Neurodegenerative Diseases
- Advanced Electron Microscopy Techniques and Applications
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Neural dynamics and brain function
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
The Netherlands Cancer Institute
2022-2024
Oncode Institute
2022-2024
Radboud University Nijmegen
2020-2021
Radboud University Medical Center
2020-2021
Activity in the healthy brain relies on a concerted interplay of excitation (E) and inhibition (I) via balanced synaptic communication between glutamatergic GABAergic neurons. A growing number studies imply that disruption this E/I balance is commonality many disorders; however, obtaining mechanistic insight into these disruptions, with translational value for patient, has typically been hampered by methodological limitations. Cadherin-13 (CDH13) associated autism...
The detyrosination-tyrosination cycle involves the removal and religation of C-terminal tyrosine α-tubulin is implicated in cognitive, cardiac, mitotic defects. vasohibin-small vasohibin-binding protein (SVBP) complex underlies much, but not all, detyrosination. We used haploid genetic screens to identify an unannotated protein, microtubule associated carboxypeptidase (MATCAP), as a remaining detyrosinating enzyme. X-ray crystallography cryo-electron microscopy structures established...
Summary Activity in the healthy brain relies on concerted interplay of excitation (E) and inhibition (I) via balanced synaptic communication between glutamatergic GABAergic neurons. A growing number studies imply that disruption this E/I balance is a commonality many disorders, however, obtaining mechanistic insight into these disruptions, with translational value for human patient, has typically been hampered by methodological limitations. Cadherin-13 ( CDH13 ) strongly associated to...
Microtubules are a major component of the cytoskeleton and can accumulate plethora modifications. The microtubule detyrosination cycle is one these modifications; it involves enzymatic removal C‐terminal tyrosine α‐tubulin on assembled microtubules re‐ligation detyrosinated tubulin dimers. This modification has been implicated in cardiac disease, neuronal development, mitotic defects. vasohibin microtubule‐associated carboxypeptidase enzyme families responsible for detyrosination. Their...
Most proteins consist of both folded domains and Intrinsically Disordered Regions (IDRs). However, the widespread occurrence intrinsic disorder in human proteins, along with its characteristics, is often overlooked by broader communities structural molecular biologists. Building on MobiDB database intrinsically here we develop a comprehensive dataset (Comprehensive analysis Human And their disOrdered Segments (CHAOS)). We implement empirical internally consistent definitions what constitutes...
Microtubules are an important part of the cytoskeleton and involved in intracellular organization, cell division, migration. Depending on posttranslational modifications, microtubules can form complexes with various interacting proteins. These microtubule-protein often implicated human diseases. Understanding structure such is useful for elucidating their mechanisms action be studied by cryo-electron microscopy (cryo-EM). To obtain structural studies, it to extract containing or lacking...
Microtubules are an important part of the cytoskeleton and involved in intracellular organization, cell division, migration. Depending on posttranslational modifications, microtubules can form complexes with various interacting proteins. These microtubule-protein often implicated human diseases. Understanding structure such is useful for elucidating their mechanisms action be studied by cryo-electron microscopy (cryo-EM). To obtain structural studies, it to extract containing or lacking...