A5018851180- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Congenital heart defects research
- Spaceflight effects on biology
- Neurogenetic and Muscular Disorders Research
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Exercise and Physiological Responses
- Cardiovascular Effects of Exercise
- Circadian rhythm and melatonin
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Ion channel regulation and function
- Renal and related cancers
- Electrospun Nanofibers in Biomedical Applications
- EFL/ESL Teaching and Learning
- Second Language Learning and Teaching
- CRISPR and Genetic Engineering
University of Minnesota Medical Center
2012-2025
University of Minnesota
2016-2025
Stem Cell Institute
2010-2017
Muscular Dystrophy Association
2007-2017
Nagoya University
2014-2016
St. Anne's University Hospital Brno
2016
University Hospital Brno
2016
Twin Cities Orthopedics
2010
GTx (United States)
2008
Stem Cell Network
2007
Skeletal muscle contains myogenic progenitors called satellite cells and muscle-derived stem that have been suggested to be pluripotent. We further investigated the differentiation potential of elucidate relationships between these two populations cells. FACS® analysis side population (SP) cells, a fraction revealed expression hematopoietic cell marker Sca-1 but did not reveal any markers. Muscle SP were greatly enriched for competent form colonies. Moreover, with CD45 positive. However,...
d Notch signaling is a conserved cell fate regulator during development and postnatal tissue regeneration.Using skeletal muscle satellite cells as model through myogenic lineage-specific NICD OE (overexpression of constitutively activated 1 intracellular domain), here we investigate how regulates the choice stem cells.We show that in addition to inhibiting MyoD differentiation, upregulates Pax7 promotes self-renewal cell-derived primary myoblasts culture.Using ؊/؊ myoblasts, further...
To gain insight into the regeneration deficit of MyoD−/− muscle, we investigated growth and differentiation cultured myogenic cells. Primary cells exhibited a stellate morphology distinct from compact wild-type myoblasts, expressed c-met, receptor tyrosine kinase in satellite However, did not express desmin, an intermediate filament protein typically myoblasts vitro precursor vivo. Northern analysis indicated that proliferating fourfold higher levels Myf-5 sixfold PEA3, ETS-domain...
ABSTRACT The myogenic progenitors of epaxial (paraspinal and intercostal) hypaxial (limb abdominal wall) musculature are believed to originate in dorsal-medial ventral-lateral domains, respectively, the developing somite. To investigate hypothesis that Myf-5 MyoD have different roles development musculature, we further characterized myogenesis Myf-5- MyoD-deficient embryos by several approaches. We examined expression a MyoD-lacZ transgene mutant characterize temporal-spatial patterns...
The molecules that regulate the apoptosis cascade are also involved in differentiation and syncytial fusion skeletal muscle. MyoD is a myogenic transcription factor plays essential roles muscle differentiation. We noticed MyoD−/− myoblasts display remarkable resistance to by down-regulation of miR-1 (microRNA-1) miR-206 up-regulation Pax3. This resulted transcriptional activation antiapoptotic factors Bcl-2 Bcl-xL. Forced expression induces Pax3, Bcl-2, Bcl-xL along with increased myoblasts....
Muscle satellite cells are a stem cell population required for postnatal skeletal muscle development and regeneration, accounting 2-5% of sublaminal nuclei in fibers. In adult muscle, normally mitotically quiescent. Following injury, however, initiate cellular proliferation to produce myoblasts, their progenies, mediate the regeneration muscle. Transplantation cell-derived myoblasts has been widely studied as possible therapy several regenerative diseases including muscular dystrophy, heart...
We have identified two new genes, neuroD2 and neuroD3, on the basis of their similarity to neurogenic basic-helix-loop-helix (bHLH) gene neuroD. The predicted amino acid sequence shows a high degree homology neuroD MATH-2/NEX-1 in bHLH region, whereas neuroD3 is more distantly related family member. expressed transiently during embryonic development, with highest levels expression between days 10 12. initially at day 11, persistent adult nervous system. In situ Northern (RNA) analyses...
MyoD and Myf5 are basic helix-loop-helix transcription factors that play key but redundant roles in specifying myogenic progenitors during embryogenesis. However, there functional differences between the two impact myoblast proliferation differentiation. Target gene activation could be one such difference. We have used microarray polymerase chain reaction approaches to measure induction of muscle expression by an vitro model. In proliferating cells, function very similarly activate likely...
MyoD is a myogenic master transcription factor that plays an essential role in muscle satellite cell (muscle stem cell) differentiation. To further investigate the function of cells, we examined transplantation cell-derived myoblasts lacking gene into regenerating skeletal muscle. After injection injured muscle, MyoD(-/-) engrafted with significantly higher efficiency compared wild-type myoblasts. In addition, myoblast-derived cells were detected underneath basal lamina fibers, indicating...
Brown adipose tissue (BAT) represents a promising agent to ameliorate obesity and other metabolic disorders. However, the abundance of BAT decreases with age paucity is common feature obese subjects. As brown adipocytes myoblasts share Myf5 lineage origin, elucidating molecular mechanisms underlying fate choices versus may lead novel approaches expand mass. Here we identify MyoD as key negative regulator adipocyte development. CRISPR/CAS9-mediated deletion in C2C12 facilitates their...
Endothelial and skeletal muscle lineages arise from common embryonic progenitors. Despite their shared developmental origin, adult endothelial cells (ECs) stem (MuSCs; satellite cells) have been thought to possess distinct gene signatures signaling pathways. Here, we shift this paradigm by uncovering how MuSC behavior is affected the expression of a subset EC transcripts. We used several computational analyses including single-cell RNA-seq (scRNA-seq) show that MuSCs express low levels...
Abstract Swine-derived MSCs were efficiently isolated and extensively expanded using a low fetal serum content growth medium to which selected factors added. After ≥96 cell population doublings (PDs), devoid of cytogenetic abnormalities. In vitro chondrogenic osteogenic differentiation capacity was preserved after 80 PDs. To test therapeutic efficacy, 1 × 106 80-PD injected directly into the peri-infarct zone hearts immunodeficient (non-obese diabetic/severe combined immunodeficient) mice at...
Abstract A novel population of tissue-resident endothelial precursors (TEPs) was isolated from small blood vessels in dermal, adipose, and skeletal muscle mouse based on their ability to be grown as spheres. Cellular molecular analyses these cells revealed that they were highly related regardless the tissue origin distinct embryonic neural stem cells. Notably, TEPs did not express hematopoietic markers, but expressed numerous characteristics angiogenic differentiated progeny, such CD34,...
Circadian rhythms regulate cell proliferation and differentiation; however, little is known about their roles in myogenic differentiation. Our synchronized differentiation studies demonstrate that myoblast subsequent myotube formation by fusion occur circadian manners. We found one of the core regulators rhythms, Cry2, but not Cry1, critical for patterns these two steps This achieved through specific interaction between Cry2 Bclaf1, which stabilizes mRNAs encoding cyclin D1, a G1/S phase...