A5042286795- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Protein Structure and Dynamics
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Microbial Fuel Cells and Bioremediation
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
University of Stuttgart
2014-2021
Acetogens such as Clostridium ljungdahlii can play a crucial role reducing the human CO
In vivo protein formation is a crucial part of cellular life. The process needs to adapt growth conditions and exploited for the production technical pharmaceutical proteins in microbes such as Escherichia coli. Accordingly, elucidation basic regulatory mechanisms controlling translation machinery primary interest, not only improve heterologous but also elucidate fundamental regulation regimens growth. current modeling analysis elucidates impact diffusion stochastic supply substrates...
Corynebacterium glutamicum is a commonly applied host for the industrial production of amino acids. While valued its robustness, it somewhat inferior to competing strains such as Escherichia coli because relatively low growth rate 0.40 h-1 in synthetic, media. Accordingly, adaptive laboratory evolution (ALE) experiments were performed continuous cultivation mode select growth-improved host. To ensure attractiveness, this ALE study aimed at reduction dependency on costly growth-boosting...
Cell-free (in vitro) protein synthesis (CFPS) systems provide a versatile tool that can be used to investigate different aspects of the transcription-translation machinery by reducing cells basic functions formation. Recent improvements in reaction stability and lysate preparation offer potential expand scope vitro biosynthesis from research multifunctional platform for production synthetic biology. To date, even best-performing CFPS are drastically slower than vivo references. Major...
Rapidly changing concentrations of substrates frequently occur during large-scale microbial cultivations. These conditions, caused by large mixing times, result in a heterogeneous population distribution. Here, we present powerful and efficient modeling approach to predict the influence varying substrate levels on transcriptional translational response cell. This consists two parts, single-cell model describe transcription translation for an exemplary operon (trp operon) second part...
Ribosomes are a crucial component of the physiological state cell. Therefore, we aimed to monitor ribosome dynamics using fast and easy fluorescence readout. Using fluorescent-labeled ribosomal proteins, ribosomes during batch cultivation nutritional shift conditions was investigated. The readout compared cellular rRNA content determined by capillary gel electrophoresis with laser-induced detection exponentially accelerating decelerating growth. We found linear correlation between observed...
To ensure economic competitiveness, bioprocesses should achieve maximum productivities enabled by high growth rates (μ) and equally substrate consumption (qS) as a prerequisite of sufficient carbon-to-product conversion. Both traits were investigated improved via bioprocess engineering approaches studying the industrial work horse Corynebacterium glutamicum. Standard minimal medium CGXII with glucose sole carbon source was supplemented complex brain-heart-infusion (BHI) or amino acid (AA)...
Clostridium ljungdahlii ( C. , CLJU) is natively endowed producing acetic acid, 2,3-butandiol, and ethanol consuming gas mixtures of CO 2 CO, H (syngas). Here, we present the syngas-based isobutanol formation using harboring recombinant amplification “Ehrlich” pathway that converts intracellular KIV to isobutanol. Autotrophic production was studied analyzing two different strains in 3-L gassed stirred bioreactors. Physiological characterization thoroughly applied together with metabolic...
The growth rate (µ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying imperative for improving productivity and performance through metabolic engineering. Despite recent progress in global regulatory interactions, knowledge mechanisms directing cell remains fragmented incomplete. current study investigated RNA-Seq data three transitions, induced by...